HRP Anti-M13 antibody [B62-FE2] (ab50370)

Reviews (2)Q&A (5)References (8)
ELISA - HRP Anti-M13 antibody [B62-FE2] (ab50370)

    Key features and details

    • HRP Mouse monoclonal [B62-FE2] to M13
    • Suitable for: ELISA
    • Reacts with: Species independent
    • Conjugation: HRP
    • Isotype: IgG2b

    Overview

    • Product name

      HRP Anti-M13 antibody [B62-FE2]
      See all M13 primary antibodies

    • Description

      HRP Mouse monoclonal [B62-FE2] to M13

    • Host species

      Mouse

    • Conjugation

      HRP

    • Tested applications

      Suitable for: ELISAmore details

    • Species reactivity

      Reacts with: Species independent

    • Immunogen

      ab50370 was raised using fd phages from E. Coli F+ strain (JM109).

    • Epitope

      ab50370 binds to an epitope on pVIII (phage coat protein) covering the N-Terminal region of the g8p AEGDDPAKAAFDSLQASAT.

    • General notes

      Strongly recommend adding 0.5% BSA to diluents and a suitable preservative (as 0.05-0.09% sodium azide or equivalent) for extra stablity when stored at 2-8 °C

      Filamentous fd bacteriophages are used in immunoassays for the identification of recombinant antigen- or antibody phages. They have also been used to construct phage-display peptide libraries, which have been useful in selecting peptides that interact with specific domains within target molecules.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 7.40
      Constituents: PBS, 0.1% BSA
    • Concentration information loading...

    • Purity

      Protein A purified

    • Primary antibody notes

      Filamentous fd bacteriophages are used in immunoassays for the identification of recombinant antigen- or antibody phages. They have also been used to construct phage-display peptide libraries, which have been useful in selecting peptides that interact with specific domains within target molecules.

    • Clonality

      Monoclonal

    • Clone number

      B62-FE2

    • Isotype

      IgG2b

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab50370 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA (2)
    1/20.

    ab50370 can be used in Phage Display (immunoassays for the identification of recombinant antigen or antibody-phages) (Detection Limit: 107 phage particles).
    Notes
    ELISA
    1/20.

    ab50370 can be used in Phage Display (immunoassays for the identification of recombinant antigen or antibody-phages) (Detection Limit: 107 phage particles).

    Target

    • Relevance

      The bacteriophage known as "M13" forms the basis of cloning systems designed to easily introduce mutations into genes inserted into the phage genome. It also has been used in various "phage display" methodologies and combinatorial DNA and peptide libraries. Coat protein A (G3P) is necessary for adsorption of the virion onto the F-pilus of the host cell. There are about five copies of this protein per mature phage. Consists of three domains (N1, N2, and CT). The N2 domain interacts with the F pilus, whereas the N1 domain (connected to N2 by a flexible glycine-rich linker and tightly interacting with it on the phage) forms a complex with the C-terminal domain of tolA at later stages of the infection process. They are located at the adsorption end of the phage particle.

    • Alternative names

      • Bacteriophage M13 antibody
      • Coliphage M13 antibody
      • Enterobacteria phage M13 antibody
      • M13 virus antibody
      • Phage M13 antibody
      see all

    Images

    • ELISA - HRP Anti-M13 antibody [B62-FE2] (ab50370)
      ELISA - HRP Anti-M13 antibody [B62-FE2] (ab50370)This image is courtesy of an anonymous Abreview

      ab50370 detecting M13 phage. Samples were blocked with 1% BSA for 2 hours at 25°C and incubated with the primary antibody (1/1000 in 50mM carbonate/bicarbonate buffer, pH 9) for 12 hours at 4°C.

      See Abreview

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (8)

    Publishing research using ab50370? Please let us know so that we can cite the reference in this datasheet.

    ab50370 has been referenced in 8 publications.

    • Dong Y  et al. Construction and application of a human scFv phage display library based on Cre-LoxP recombination for anti-PCSK9 antibody selection. Int J Mol Med 47:708-718 (2021). PubMed: 33416098
    • Oo KK  et al. Development of an engineered peptide antagonist against periostin to overcome doxorubicin resistance in breast cancer. BMC Cancer 21:65 (2021). PubMed: 33446140
    • Wang Q  et al. Identification of a sodium pump Na+/K+ ATPase a1-targeted peptide for PET imaging of breast cancer. J Control Release 281:178-188 (2018). ELISA ; Mouse . PubMed: 29777796
    • Kang Y  et al. Cryo-EM structure of human rhodopsin bound to an inhibitory G protein. Nature 558:553-558 (2018). PubMed: 29899450
    • Jung HS  et al. Monoclonal antibodies against autocrine motility factor suppress gastric cancer. Oncol Lett 13:4925-4932 (2017). PubMed: 28599497
    • Shanmugam A  et al. Synthetic Toll like receptor-4 (TLR-4) agonist peptides as a novel class of adjuvants. PLoS One 7:e30839 (2012). ELISA . PubMed: 22363498
    • Dudak FC  et al. Thermodynamic and structural analysis of interactions between peptide ligands and SEB. J Mol Recognit 23:369-78 (2010). PubMed: 19941320
    • Soykut EA  et al. Selection of staphylococcal enterotoxin B (SEB)-binding peptide using phage display technology. Biochem Biophys Res Commun 370:104-8 (2008). PubMed: 18359289

    Customer reviews and Q&As

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    1-7 of 7 Abreviews or Q&A

    Question

    I have recently purchased product ab50370, Anti-M13 antibody-HRP, for use in phage display ELISA.
    I note that the product datasheet recommends using this in ELISA at a dilution of 1/20.
    Can you tell me, is this a misprint? Such a dilution would make this product useless for ELISA, or at least the 250µl unit would only allow half an ELISA plate to be run!
    Comparable products normally are diluted at greater than 1/2000. For example, the comparable product from GE Healthcare (Cat # 27-9421-01) is used at 1/5000 dilution.
    Any clarification on your recommended dilution would be welcomed.

    Read More
    Answer

    Thank you for contacting us.

    The dilution of 1/20 is correct. 250 µl should be used in 5 mL of working solution. When using a volume of 100 µl/well the product allows for testing of 50 wells.

    Please note, the recommended dilutions are for guideline purpose only. The optimal dilution should be worked out using different dilutions of product.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    ELISA abreview for Anti-M13 antibody [B62-FE2] (HRP)

     Excellent
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Recombinant protein (Antigen)
    Specification
    Antigen
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
    Type
    Direct
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 11 2018

    ELISA abreview for Anti-M13 antibody [B62-FE2] (HRP)

     Excellent
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Mouse Cell (M13 KE bacteriophage)
    Specification
    M13 KE bacteriophage
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Type
    Sandwich (Detection)
    Read More

    Xiujuan Peng

    Verified customer

    Submitted Oct 18 2016

    Question

    I am doing an experiment about phage display. I need an HRP/anti-M13 antibody for ELISA, but I do not know which antibody to choose.

    Read More
    Answer

    It appears that ab50370 would be best suited to your needs as it is both HRP conjugated and has been tested and shown to work in ELISA.

    Read More

    Question

    Inquiry: Hi, Can you use Anti-M13 antibody [B62-FE2] (HRP) (ab50370) for Western application to detect M13 phage? Thanks

    Read More
    Answer

    Thank you for contacting us and for your interest in our products.

    I am sorry to confirm that as far as we are aware, ab50370 has never been tested in WB . All tested applications covered by our 6 month guaranteeare specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

    We do have the following M13 antibodies tested in WB that I hope may be helpful to you. I can recommend to reveiw ths online datasheets for further information and to ensure they will be suitable for your experiments:

    ab24229 Anti-M13 antibody [E1]
    Tested in: WB, ELISA
    https://www.abcam.com/M13-antibody-E1-ab24229.html (or use the following: https://www.abcam.com/M13-antibody-E1-ab24229.html).

    ab17269Anti-M13 antibody [E1] (Biotin)
    Tested in: Flow Cyt, WB, ELISA
    https://www.abcam.com/Biotin-M13-antibody-E1-ab17269.html (or use the following: https://www.abcam.com/Biotin-M13-antibody-E1-ab17269.html).


    If you would like to test ab50370 in WB, please do not hesitate to contact me again prior to the purchase by replying to this message as you may be eligible for our testing discount program.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

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    Question

    Thank you very much for reply. So do you supply the anti-pVIII protein of M13 phage antibody for ELISA (used in phage display experiment)? And HRP/AntiM13 and HRP substrate OPD (O-phenyldiamine dihydrochloride)?   Thank you very much.  

    Read More
    Answer

    Thank you for contacting us. We have two anti-M13 antibodies that may be of interest: ab6188 Anti-M13 + Fd Bacteriophage Coat Proteins antibody Binds specifically to phage coat proteins of M13 or fd phage. Click here (or use the following: https://www.abcam.com/M13--Fd-Bacteriophage-Coat-Proteins-antibody-ab6188.html). ab50370 Anti-M13 antibody [B62-FE2] (HRP) Binds to an epitope on pVIII (phage coat protein) covering the N-Terminal region of the g8p AEGDDPAKAAFDSLQASAT. Click here (or use the following: https://www.abcam.com/HRP-M13-antibody-B62-FE2-ab50370.html). We have a number of HRP kits, mainly for IHC. Here is a link to a HRP substrate than can be used for WB: ab5801 Chemiluminescent HRP Substrate (membrane) Click here (or use the following: https://www.abcam.com/Chemiluminescent-HRP-Substrate-membrane-ab5801.html). Please let me know if there is anything else I can help you with.

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    Question

    Thank you very much for your prompt reply.  Just one quick question, do you have the antibody for pVIII coat protein of the M13 phage?

    Read More
    Answer

    Thank you for your reply. Our HRP-conjugated M13 antibody ab50370 binds to an epitope on pVIII covering the N-Terminal region of the g8p AEGDDPAKAAFDSLQASAT. I hope this helps, please let me know if you need any additional information.

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