Key features and details
- HRP Mouse monoclonal [B62-FE2] to M13
- Suitable for: ELISA
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG2b
Product nameHRP Anti-M13 antibody [B62-FE2]
See all M13 primary antibodies
DescriptionHRP Mouse monoclonal [B62-FE2] to M13
Tested applicationsSuitable for: ELISAmore details
Species reactivityReacts with: Species independent
ab50370 was raised using fd phages from E. Coli F+ strain (JM109).
Epitopeab50370 binds to an epitope on pVIII (phage coat protein) covering the N-Terminal region of the g8p AEGDDPAKAAFDSLQASAT.
Strongly recommend adding 0.5% BSA to diluents and a suitable preservative (as 0.05-0.09% sodium azide or equivalent) for extra stablity when stored at 2-8 °C
Filamentous fd bacteriophages are used in immunoassays for the identification of recombinant antigen- or antibody phages. They have also been used to construct phage-display peptide libraries, which have been useful in selecting peptides that interact with specific domains within target molecules.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.40
Constituents: PBS, 0.1% BSA
Concentration information loading...
PurityProtein A purified
Primary antibody notesFilamentous fd bacteriophages are used in immunoassays for the identification of recombinant antigen- or antibody phages. They have also been used to construct phage-display peptide libraries, which have been useful in selecting peptides that interact with specific domains within target molecules.
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab50370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab50370 can be used in Phage Display (immunoassays for the identification of recombinant antigen or antibody-phages) (Detection Limit: 107 phage particles).
RelevanceThe bacteriophage known as "M13" forms the basis of cloning systems designed to easily introduce mutations into genes inserted into the phage genome. It also has been used in various "phage display" methodologies and combinatorial DNA and peptide libraries. Coat protein A (G3P) is necessary for adsorption of the virion onto the F-pilus of the host cell. There are about five copies of this protein per mature phage. Consists of three domains (N1, N2, and CT). The N2 domain interacts with the F pilus, whereas the N1 domain (connected to N2 by a flexible glycine-rich linker and tightly interacting with it on the phage) forms a complex with the C-terminal domain of tolA at later stages of the infection process. They are located at the adsorption end of the phage particle.
- Bacteriophage M13 antibody
- Coliphage M13 antibody
- Enterobacteria phage M13 antibody
ab50370 detecting M13 phage. Samples were blocked with 1% BSA for 2 hours at 25°C and incubated with the primary antibody (1/1000 in 50mM carbonate/bicarbonate buffer, pH 9) for 12 hours at 4°C.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab50370 has been referenced in 6 publications.
- Wang Q et al. Identification of a sodium pump Na+/K+ ATPase a1-targeted peptide for PET imaging of breast cancer. J Control Release 281:178-188 (2018). ELISA ; Mouse . PubMed: 29777796
- Kang Y et al. Cryo-EM structure of human rhodopsin bound to an inhibitory G protein. Nature 558:553-558 (2018). PubMed: 29899450
- Jung HS et al. Monoclonal antibodies against autocrine motility factor suppress gastric cancer. Oncol Lett 13:4925-4932 (2017). PubMed: 28599497
- Shanmugam A et al. Synthetic Toll like receptor-4 (TLR-4) agonist peptides as a novel class of adjuvants. PLoS One 7:e30839 (2012). ELISA . PubMed: 22363498
- Dudak FC et al. Thermodynamic and structural analysis of interactions between peptide ligands and SEB. J Mol Recognit 23:369-78 (2010). PubMed: 19941320
- Soykut EA et al. Selection of staphylococcal enterotoxin B (SEB)-binding peptide using phage display technology. Biochem Biophys Res Commun 370:104-8 (2008). PubMed: 18359289