Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR2425Y] to Metabotropic Glutamate Receptor 5
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: HRP
Product nameHRP Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y]
See all Metabotropic Glutamate Receptor 5 primary antibodies
DescriptionHRP Rabbit monoclonal [EPR2425Y] to Metabotropic Glutamate Receptor 5
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human Metabotropic Glutamate Receptor 5 aa 1150 to the C-terminus (C terminal). The exact sequence is proprietary.
(Peptide available as
- IHC-P: Normal human cerebellum tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Alexa Fluor® 488 Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab196481)
- Alexa Fluor® 647 Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab196929)
- Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374)
- Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - BSA and Azide free (ab256589)
- Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
Our Abpromise guarantee covers the use of ab196482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab199507 - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody.
FunctionG-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
Sequence similaritiesBelongs to the G-protein coupled receptor 3 family.
Cellular localizationCell membrane.
- Information by UniProt
- Glutamate receptor metabotropic 5 antibody
- GPRC1E antibody
- Grm5 antibody
IHC image of Metabotropic Glutamate Receptor 5 staining in a section of formalin-fixed paraffin-embedded normal human cerebellum, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196482 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab196482 has not yet been referenced specifically in any publications.