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Epigenetics and Nuclear Signaling Histones H3 Methylated
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HRP Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23367)

  • Datasheet
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Find out more.

Key features and details

  • HRP Rabbit polyclonal to Methylated Lysine (di methyl , mono methyl )
  • Suitable for: ELISA, WB
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG

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Conjugation
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HRP Conjugation Kit - Lightning-Link® (ab102890)
Primary
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Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23366)
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Recombinant human HGF protein (Active) (ab632)

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Overview

  • Product name

    HRP Anti-Methylated Lysine (di methyl , mono methyl ) antibody
    See all Methylated Lysine primary antibodies
  • Description

    HRP Rabbit polyclonal to Methylated Lysine (di methyl , mono methyl )
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Specificity

    Recognises proteins methylated on lysine residues (mono and dimethy-lysine). Does not cross react with acetylated proteins.
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Methylated KLH conjugates.

  • General notes

    This antibody can be used for detection, quantification and isolation of proteins containing methylated lysine residues. Direct labelling of this primary antibody with HRP means that secondary antibody conjugates are not required.

    Reductive amination with HRP to antibody molar ratio of 2:1.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 6
    Constituents: 0.268% PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Affinity purified using methyl-lysine and di-methyl-lysine on agarose
  • Primary antibody notes

    This antibody can be used for detection, quantification and isolation of proteins containing methylated lysine residues. Direct labelling of this primary antibody with HRP means that secondary antibody conjugates are not required.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Methylated
    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Epigenetics and Nuclear Signaling
    • Histones
    • H2A
    • Methylated
    • Epigenetics and Nuclear Signaling
    • Histones
    • H4
    • Methylated
    • Epigenetics and Nuclear Signaling
    • Histones
    • H2B
    • Methylated
    • Cell Biology
    • Other Antibodies
    • Other Antibodies
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Related Products

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 450 nm Stop Solution for TMB Substrate (ab171529)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)

Applications

Our Abpromise guarantee covers the use of ab23367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

Target

  • Relevance

    Many proteins are post translationally modified. Modifications such as phosphorylation, glycosylation, ubiquitination and methylation have been shown to play an important role in the development, physiology and disease of animals and plants. Histone lysine methylation is regarded as a very stable modification with important functions in epigenetic gene control and for organizing chromatin domains.
  • Alternative names

    • Dimethylysine antibody
    • Methyl lysine antibody
    • N epsilon dimethyl lysine antibody
    • N epsilon mono methyllysine antibody
    • N epsilon trimethyl lysine antibody
    • N(epsilon) trimethyl lysine antibody
    • Trimethyl lysine antibody
    see all

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab23367? Please let us know so that we can cite the reference in this datasheet.

    ab23367 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Thank you for your kind help. I have an inquiry about your products, ab23366, ab23367 and ab26333. One of our customers purchased them. Are all of these antibody same except labeled substances?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 14 2006

    Answer

    Thank you for your enquiry. Ab23366 and ab23367 are the same antibody except that ab23367 is the HRP-conjugated version of ab23366. Ab26333 is obtained from a different source than ab23366 and ab23367, and it is not the same as the other two. Ab26333 also recognizes methylated lysine and it is biotin-conjugated. Please contact us again if you have any additional questions.

    Read More

    Abcam Scientific Support

    Answered on Apr 14 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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