RecombinantRabMAb

Recombinant HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)

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Western blot - HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)
  • Dot Blot - HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)
  • HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR9515(2)] to MLKL (phospho S345)
  • Suitable for: Dot blot, WB
  • Reacts with: Mouse
  • Conjugation: HRP

Conjugates logo Related conjugates and formulations

Carrier Free Unconjugated

Overview

  • Product name

    HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)]
    See all MLKL primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR9515(2)] to MLKL (phospho S345)

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Specificity

    MLKL pS345 is a trigger for necroptosis. It is only detectable in infection/cellular damaged (PMID:29229989) or aging tissue (PMID: 28807105) but not in normal tissues.

  • Tested applications

    Suitable for: Dot blot, WBmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • L-929 cells.

  • General notes

    This antibody was developed through collaboration with the lab of Xiaodong Wang at the National Institute of Biological Sciences, Beijing.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab208430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
1/1000.
WB
1/4500. Predicted molecular weight: 54 kDa.
Notes
Dot blot
1/1000.
WB
1/4500. Predicted molecular weight: 54 kDa.

Target

  • Sequence similarities

    Belongs to the protein kinase superfamily.
    Contains 1 protein kinase domain.

  • Domain

    The protein kinase domain is predicted to be catalytically inactive.
  • Information by UniProt

  • Database links

    • Alternative names

      • 9130019I15Rik antibody
      • FLJ34389 antibody
      • hMLKL antibody
      • Mixed lineage kinase domain like antibody
      • Mixed lineage kinase domain like protein antibody
      • Mixed lineage kinase domain like pseudokinase antibody
      • Mixed lineage kinase domain-like protein antibody
      • Mlkl antibody
      • MLKL_HUMAN antibody
      see all

    Images

    • Western blot - HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)
      Western blot - HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)
      All lanes : HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430) at 1/4500 dilution

      Lane 1 : Untreated L-929 (Mouse connective tissue fibroblast cell line) whole cell lysates
      Lane 2 : L-929 (Mouse connective tissue fibroblast cell line) treated with 20 ng/ml TNF alpha,100nM Smac mimetic , and 20mM z-VAD for 8 hours whole cell lysates
      Lane 3 : L-929 (Mouse connective tissue fibroblast cell line) treated with 20 ng/ml TNF alpha, 100nM Smac mimetic , and 20mM z-VAD for 8 hours whole cell lysates. Then the membrane was incubated with Alkaline phosphatase.

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 54 kDa
      Observed band size: 54 kDa


      Exposure time: 1 minute


      Blocking and diluting buffer: 5% NFDM/TBST.

    • Dot Blot - HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)
      Dot Blot - HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)

      Dot Blot analysis of

      Lane 1: MLKL (phospho S345) phospho peptide and

      Lane 2: MLKL Non-phospho peptide labeling MLKL (phospho S345)

      with ab208430 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. Exposure time: 3 minutes.

    • HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)
      HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)

    References (0)

    Publishing research using ab208430? Please let us know so that we can cite the reference in this datasheet.

    ab208430 has not yet been referenced specifically in any publications.

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