Recombinant HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)
![Western blot - HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)](https://www.abcam.com/ps/products/208/ab208430/Images/ab208430-268657-anti-mlkl-phospho-s345-antibody-epr95152-hrp-western-blot.jpg "Western blot - HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR9515(2)] to MLKL (phospho S345)
- Suitable for: Dot blot, WB
- Reacts with: Mouse
- Conjugation: HRP
Overview
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Product name
HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)]
See all MLKL primary antibodies -
Description
HRP Rabbit monoclonal [EPR9515(2)] to MLKL (phospho S345) -
Host species
Rabbit -
Conjugation
HRP -
Specificity
MLKL pS345 is a trigger for necroptosis. It is only detectable in infection/cellular damaged (PMID:29229989) or aging tissue (PMID: 28807105) but not in normal tissues. -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- L-929 cells.
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General notes
This antibody was developed through collaboration with the lab of Xiaodong Wang at the National Institute of Biological Sciences, Beijing.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9515(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab208430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot |
1/1000.
|
|
| WB |
1/4500. Predicted molecular weight: 54 kDa.
|
| Notes |
|---|
|
Dot blot
1/1000. |
|
WB
1/4500. Predicted molecular weight: 54 kDa. |
Target
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Sequence similarities
Belongs to the protein kinase superfamily.
Contains 1 protein kinase domain. -
Domain
The protein kinase domain is predicted to be catalytically inactive. - Information by UniProt
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Database links
- Entrez Gene: 74568 Mouse
- SwissProt: Q9D2Y4 Mouse
- Unigene: 207971 Mouse
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Alternative names
- 9130019I15Rik antibody
- FLJ34389 antibody
- hMLKL antibody
see all
Images
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Western blot - HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)All lanes : HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430) at 1/4500 dilution
Lane 1 : Untreated L-929 (Mouse connective tissue fibroblast cell line) whole cell lysates
Lane 2 : L-929 (Mouse connective tissue fibroblast cell line) treated with 20 ng/ml TNF alpha,100nM Smac mimetic , and 20mM z-VAD for 8 hours whole cell lysates
Lane 3 : L-929 (Mouse connective tissue fibroblast cell line) treated with 20 ng/ml TNF alpha, 100nM Smac mimetic , and 20mM z-VAD for 8 hours whole cell lysates. Then the membrane was incubated with Alkaline phosphatase.
Lysates/proteins at 15 µg per lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 1 minuteBlocking and diluting buffer: 5% NFDM/TBST.
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![Dot Blot - HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)](https://www.abcam.com/ps/products/208/ab208430/Images/ab208430-268658-anti-mlkl-phospho-s345-antibody-epr95152-hrp-dot-blot.jpg)
Dot Blot - HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)Dot Blot analysis of
Lane 1: MLKL (phospho S345) phospho peptide and
Lane 2: MLKL Non-phospho peptide labeling MLKL (phospho S345)
with ab208430 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. Exposure time: 3 minutes.
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![HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)](https://www.abcam.com/ps/products/208/ab208430/Images/ab208430-3-benefits-of-recombinant-antibodies.png)
HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab208430 has not yet been referenced specifically in any publications.