RecombinantRabMAb

Recombinant HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)

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Western blot - HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)
  • Dot Blot - HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)
  • HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR9515(2)] to MLKL (phospho S345)
  • Suitable for: Dot blot, WB
  • Reacts with: Mouse
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)]
    See all MLKL primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR9515(2)] to MLKL (phospho S345)

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Specificity

    MLKL pS345 is a trigger for necroptosis. It is only detectable in infection/cellular damaged (PMID:29229989) or aging tissue (PMID: 28807105) but not in normal tissues.

  • Tested applications

    Suitable for: Dot blot, WBmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Mouse MLKL aa 300-400 (phospho S345). The exact sequence is proprietary.
    Database link: Q8NB16

  • Positive control

    • L-929 cells.

  • General notes

    This antibody was developed through collaboration with the lab of Xiaodong Wang at the National Institute of Biological Sciences, Beijing.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: PBS, 1% BSA, 30% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR9515(2)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab208430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/1000.
WB 1/4500. Predicted molecular weight: 54 kDa.

Target

  • Sequence similarities

    Belongs to the protein kinase superfamily.
    Contains 1 protein kinase domain.

  • Domain

    The protein kinase domain is predicted to be catalytically inactive.
  • Information by UniProt

  • Database links

    • Alternative names

      • 9130019I15Rik antibody
      • FLJ34389 antibody
      • hMLKL antibody
      • Mixed lineage kinase domain like antibody
      • Mixed lineage kinase domain like protein antibody
      • Mixed lineage kinase domain like pseudokinase antibody
      • Mixed lineage kinase domain-like protein antibody
      • Mlkl antibody
      • MLKL_HUMAN antibody
      see all

    Images

    • Western blot - HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)
      Western blot - HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)
      All lanes : HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430) at 1/4500 dilution

      Lane 1 : Untreated L-929 (Mouse connective tissue fibroblast cell line) whole cell lysates
      Lane 2 : L-929 (Mouse connective tissue fibroblast cell line) treated with 20 ng/ml TNF alpha,100nM Smac mimetic , and 20mM z-VAD for 8 hours whole cell lysates
      Lane 3 : L-929 (Mouse connective tissue fibroblast cell line) treated with 20 ng/ml TNF alpha, 100nM Smac mimetic , and 20mM z-VAD for 8 hours whole cell lysates. Then the membrane was incubated with Alkaline phosphatase.

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 54 kDa
      Observed band size: 54 kDa


      Exposure time: 1 minute


      Blocking and diluting buffer: 5% NFDM/TBST.

    • Dot Blot - HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)
      Dot Blot - HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)

      Dot Blot analysis of

      Lane 1: MLKL (phospho S345) phospho peptide and

      Lane 2: MLKL Non-phospho peptide labeling MLKL (phospho S345)

      with ab208430 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. Exposure time: 3 minutes.

    • HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)
      HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)

    References (0)

    Publishing research using ab208430? Please let us know so that we can cite the reference in this datasheet.

    ab208430 has not yet been referenced specifically in any publications.

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