Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR9515(2)] to MLKL (phospho S345)
- Suitable for: Dot blot, WB
- Reacts with: Mouse
- Conjugation: HRP
Product nameHRP Anti-MLKL (phospho S345) antibody [EPR9515(2)]
See all MLKL primary antibodies
DescriptionHRP Rabbit monoclonal [EPR9515(2)] to MLKL (phospho S345)
SpecificityMLKL pS345 is a trigger for necroptosis. It is only detectable in infection/cellular damaged (PMID:29229989) or aging tissue (PMID: 28807105) but not in normal tissues.
Tested applicationsSuitable for: Dot blot, WBmore details
Species reactivityReacts with: Mouse
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Mouse MLKL aa 300-400 (phospho S345). The exact sequence is proprietary.
Database link: Q8NB16
- L-929 cells.
This antibody was developed through collaboration with the lab of Xiaodong Wang at the National Institute of Biological Sciences, Beijing.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab208430 in the following tested applications.
|WB||1/4500. Predicted molecular weight: 54 kDa.|
Sequence similaritiesBelongs to the protein kinase superfamily.
Contains 1 protein kinase domain.
DomainThe protein kinase domain is predicted to be catalytically inactive.
- Information by UniProt
- 9130019I15Rik antibody
- FLJ34389 antibody
- hMLKL antibody
All lanes : HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430) at 1/4500 dilution
Lane 1 : Untreated L-929 (Mouse connective tissue fibroblast cell line) whole cell lysates
Lane 2 : L-929 (Mouse connective tissue fibroblast cell line) treated with 20 ng/ml TNF alpha,100nM Smac mimetic , and 20mM z-VAD for 8 hours whole cell lysates
Lane 3 : L-929 (Mouse connective tissue fibroblast cell line) treated with 20 ng/ml TNF alpha, 100nM Smac mimetic , and 20mM z-VAD for 8 hours whole cell lysates. Then the membrane was incubated with Alkaline phosphatase.
Lysates/proteins at 15 µg per lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 1 minute
Blocking and diluting buffer: 5% NFDM/TBST.
Dot Blot analysis of
Lane 1: MLKL (phospho S345) phospho peptide and
Lane 2: MLKL Non-phospho peptide labeling MLKL (phospho S345)
with ab208430 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. Exposure time: 3 minutes.
ab208430 has not yet been referenced specifically in any publications.