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  1. Link

    hrp-moesin-antibody-ep1863y-ab207629.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Talin / Moesin
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant HRP Anti-Moesin antibody [EP1863Y] (ab207629)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - HRP Anti-Moesin antibody [EP1863Y] (ab207629)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Moesin antibody [EP1863Y] (ab207629)
  • Western blot - HRP Anti-Moesin antibody [EP1863Y] (ab207629)
  • HRP Anti-Moesin antibody [EP1863Y] (ab207629)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EP1863Y] to Moesin
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Rat, Human
  • Conjugation: HRP

You may also be interested in

Protein
Product image
Recombinant Human Moesin protein (ab124306)

View more associated products

Overview

  • Product name

    HRP Anti-Moesin antibody [EP1863Y]
    See all Moesin primary antibodies
  • Description

    HRP Rabbit monoclonal [EP1863Y] to Moesin
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human Moesin aa 450-550. The exact sequence is proprietary.
    Database link: P26038
    (Peptide available as ab201545)

  • Positive control

    • WB: HeLa and C6 whole cell lysates. IHC-P: normal human tonsil tissue sections
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1863Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Talin / Moesin

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-Moesin antibody [EP1863Y] (ab206229)
    • PE Anti-Moesin antibody [EP1863Y] (ab210983)
    • Anti-Moesin antibody [EP1863Y] - BSA and Azide free (ab232580)
    • Anti-Moesin antibody [EP1863Y] (ab52490)
  • Isotype control

    • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)
  • Recombinant Protein

    • Recombinant Human Moesin protein (ab124306)

Applications

Our Abpromise guarantee covers the use of ab207629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 68 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Probably involved in connections of major cytoskeletal structures to the plasma membrane.
  • Tissue specificity

    In all tissues and cultured cells studied.
  • Sequence similarities

    Contains 1 FERM domain.
  • Post-translational
    modifications

    Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.
  • Cellular localization

    Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment.
  • Target information above from: UniProt accession P26038 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4478 Human
    • Entrez Gene: 17698 Mouse
    • Entrez Gene: 81521 Rat
    • Omim: 309845 Human
    • SwissProt: P26038 Human
    • SwissProt: P26041 Mouse
    • SwissProt: O35763 Rat
    • Unigene: 87752 Human
    • Unigene: 138876 Mouse
    • Unigene: 2762 Rat
    see all
  • Alternative names

    • Epididymis luminal protein 70 antibody
    • HEL70 antibody
    • Membrane organizing extension spike protein antibody
    • Membrane-organizing extension spike protein antibody
    • MOES_HUMAN antibody
    • Moesin antibody
    • Moesin/anaplastic lymphoma kinase fusion protein antibody
    • Msn antibody
    • MSN/ALK fusion antibody
    see all

Images

  • Western blot - HRP Anti-Moesin antibody [EP1863Y] (ab207629)
    Western blot - HRP Anti-Moesin antibody [EP1863Y] (ab207629)
    All lanes : HRP Anti-Moesin antibody [EP1863Y] (ab207629) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : MSN (Moesin) knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 68 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 minutes


    ab207629 was shown to specifically react with Moesin in wild-type HAP1 cells as signal was lost in MSN (Moesin) knockout cells. Wild-type and MSN (Moesin) knockout samples were subjected to SDS-PAGE. Ab207629 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Moesin antibody [EP1863Y] (ab207629)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Moesin antibody [EP1863Y] (ab207629)

    IHC image of Moesin staining in a section of formalin-fixed paraffin-embedded normal human tonsil*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab207629, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - HRP Anti-Moesin antibody [EP1863Y] (ab207629)
    Western blot - HRP Anti-Moesin antibody [EP1863Y] (ab207629)
    All lanes : HRP Anti-Moesin antibody [EP1863Y] (ab207629) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : C6 (Rat neuronal glioma tumour cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 68 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab207629 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • HRP Anti-Moesin antibody [EP1863Y] (ab207629)
    HRP Anti-Moesin antibody [EP1863Y] (ab207629)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab207629? Please let us know so that we can cite the reference in this datasheet.

    ab207629 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    There are currently no Customer reviews or Questions for ab207629.
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