RecombinantRabMAb

Recombinant HRP Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab200928)

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Western blot - HRP Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab200928)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab200928)
  • HRP Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab200928)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR7101] to Monoamine Oxidase A/MAO-A
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101]
    See all Monoamine Oxidase A/MAO-A primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR7101] to Monoamine Oxidase A/MAO-A

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    corresponding to Human Monoamine Oxidase A/MAO-A aa 450 to the C-terminus.
    Database link: P21397
    (Peptide available as ab196045)

  • Positive control

    • WB: HepG2 whole cell lysate and Human Fetal Liver tissue lysate. IHC-P: normal human kidney tissue sections

  • General notes

    Previously labelled as Monoamine Oxidase A. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab200928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.

  • Tissue specificity

    Heart, liver, duodenum, blood vessels and kidney.

  • Involvement in disease

    Defects in MAOA are the cause of Brunner syndrome (BRUNS) [MIM:300615]. Brunner syndrome is a form of X-linked non-dysmorphic mild mental retardation. Male patients are affected by a syndrome of borderline mental retardation and exhibit abnormal behavior, including disturbed regulation of impulsive aggression. Obligate female carriers have normal intelligence and behavior.

  • Sequence similarities

    Belongs to the flavin monoamine oxidase family.

  • Cellular localization

    Mitochondrion outer membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Amine oxidase [flavin containing] A antibody
    • Amine oxidase [flavin-containing] A antibody
    • AOFA antibody
    • AOFA_HUMAN antibody
    • EC 1.4.3.4 antibody
    • MAO A antibody
    • MAO-A antibody
    • maoA antibody
    • Monoamine oxidase A antibody
    • Monoamine oxidase type A antibody
    see all

Images

  • Western blot - HRP Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab200928)
    Western blot - HRP Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab200928)
    All lanes : HRP Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab200928) at 1/5000 dilution

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Liver (Human) Tissue Lysate - fetal tissue

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 60 kDa
    Observed band size: 60 kDa


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab200928 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab200928)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab200928)

    IHC image of Monoamine Oxidase A/MAO-A staining in a section of formalin-fixed paraffin-embedded normal human kidney*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab200928, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • HRP Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab200928)
    HRP Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab200928)

References (0)

Publishing research using ab200928? Please let us know so that we can cite the reference in this datasheet.

ab200928 has not yet been referenced specifically in any publications.

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