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  1. Link

    hrp-mouse-serum-albumin-antibody-ab19195.pdf

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Cardiovascular Blood Serum Proteins
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HRP Anti-Mouse Serum Albumin antibody (ab19195)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (10)

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Key features and details

  • HRP Goat polyclonal to Mouse Serum Albumin
  • Reacts with: Mouse
  • Conjugation: HRP
  • Isotype: IgG

Overview

  • Product name

    HRP Anti-Mouse Serum Albumin antibody
    See all Mouse Serum Albumin primary antibodies
  • Description

    HRP Goat polyclonal to Mouse Serum Albumin
  • Host species

    Goat
  • Conjugation

    HRP
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Full length protein (Mouse).

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 6.8
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose and conjugated to horseradish peroxidase (HRP).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Related Products

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 450 nm Stop Solution for TMB Substrate (ab171529)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)

Target

  • Function

    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificity

    Plasma.
  • Sequence similarities

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    modifications

    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P07724 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 11657 Mouse
    • SwissProt: P07724 Mouse
    • Unigene: 16773 Mouse
    • Alternative names

      • Alb 1 antibody
      • alb antibody
      • ALBU_MOUSE antibody
      • Albumin 1 antibody
      • Albumin antibody
      • Serum albumin antibody
      see all

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (10)

    Publishing research using ab19195? Please let us know so that we can cite the reference in this datasheet.

    ab19195 has been referenced in 10 publications.

    • Yang Y  et al. GSK-3 inhibitor CHIR99021 enriches glioma stem-like cells. Oncol Rep 43:1479-1490 (2020). PubMed: 32323804
    • Lu ZW  et al. Silencing of PPM1D inhibits cell proliferation and invasion through the p38 MAPK and p53 signaling pathway in papillary thyroid carcinoma. Oncol Rep 43:783-794 (2020). PubMed: 31922231
    • Lee JA  et al. Age Induced Nitroso-Redox Imbalance Leads to Subclinical Hypogonadism in Male Mice. Front Endocrinol (Lausanne) 10:190 (2019). PubMed: 30984111
    • Challa DK  et al. Neonatal Fc receptor expression in macrophages is indispensable for IgG homeostasis. MAbs 11:848-860 (2019). PubMed: 30964743
    • Grevys A  et al. A human endothelial cell-based recycling assay for screening of FcRn targeted molecules. Nat Commun 9:621 (2018). PubMed: 29434196
    • Sato Y  et al. Prolactin Upregulates Female-Predominant P450 Gene Expressions and Downregulates Male-Predominant Gene Expressions in Mouse Liver. Drug Metab Dispos 45:586-592 (2017). PubMed: 28330858
    • Swiercz R  et al. Loss of expression of the recycling receptor, FcRn, promotes tumor cell growth by increasing albumin consumption. Oncotarget 8:3528-3541 (2017). PubMed: 27974681
    • Feng T  et al. HIV-1 downregulates the expression and phosphorylation of receptor tyrosine kinase by targeting the NF-?B pathway. Mol Med Rep 14:1947-52 (2016). PubMed: 27432185
    • Jensen TB  et al. Renal type a intercalated cells contain albumin in organelles with aldosterone-regulated abundance. PLoS One 10:e0124902 (2015). Mouse . PubMed: 25874770
    • Liu H  et al. The FGL2-FcgammaRIIB pathway: a novel mechanism leading to immunosuppression. Eur J Immunol 38:3114-26 (2008). PubMed: 18991288

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    What protein should be used for the blocking to detect albumin in mouse serum by ELISA?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 18 2012

    Answer

    I would suggest to use milk as a blocking agent (0.5% Skim milk/PBS for 1 hour).

    Read More

    Abcam Scientific Support

    Answered on Oct 18 2012

    Question

    I would like to know what antibodies you have available as Western Blot loading controls for mouse serum. Antibodies against general mouse Ig have been moderately successful but we would like to consider other options.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 12 2011

    Answer

    Serum albumin may be a suitable loading control, but I am not sure if levels will vary from one animal to another, for example healthy vs. diseased. These three antibodies detect mouse serum albumin: ab19194 goat polyclonal, ab19195 (same as ab19194 but conjugated to HRP) and ab85786 rabbit polyclonal.

    Read More

    Abcam Scientific Support

    Answered on Oct 12 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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