RecombinantRabMAb

Recombinant HRP Anti-MTCO2 antibody [EPR3314] (ab200615)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-MTCO2 antibody [EPR3314] (ab200615)
  • Western blot - HRP Anti-MTCO2 antibody [EPR3314] (ab200615)
  • HRP Anti-MTCO2 antibody [EPR3314] (ab200615)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR3314] to MTCO2
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-MTCO2 antibody [EPR3314]
    See all MTCO2 primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR3314] to MTCO2

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MTCO2 aa 200 to the C-terminus.

  • Positive control

    • WB: K562, MCF7, THP1 and HeLa whole cell lysates. IHC-P: normal human liver tissue sections

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab200615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 25 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.

  • Involvement in disease

    Defects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.

  • Sequence similarities

    Belongs to the cytochrome c oxidase subunit 2 family.

  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • COII antibody
      • COX 2 antibody
      • COX II antibody
      • COX2 antibody
      • COX2_HUMAN antibody
      • COXII antibody
      • Cytochrome c oxidase II antibody
      • Cytochrome c oxidase polypeptide II antibody
      • Cytochrome c oxidase subunit 2 antibody
      • MT CO2 antibody
      • MT-CO2 antibody
      • MTCO2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-MTCO2 antibody [EPR3314] (ab200615)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-MTCO2 antibody [EPR3314] (ab200615)

      IHC image of MTCO2 staining in a section of formalin-fixed paraffin-embedded normal human liver*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab200615, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Western blot - HRP Anti-MTCO2 antibody [EPR3314] (ab200615)
      Western blot - HRP Anti-MTCO2 antibody [EPR3314] (ab200615)
      All lanes : HRP Anti-MTCO2 antibody [EPR3314] (ab200615) at 1/5000 dilution

      Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
      Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
      Lane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
      Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 25 kDa
      Observed band size: 21 kDa
      why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab200615 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • HRP Anti-MTCO2 antibody [EPR3314] (ab200615)
      HRP Anti-MTCO2 antibody [EPR3314] (ab200615)

    References (0)

    Publishing research using ab200615? Please let us know so that we can cite the reference in this datasheet.

    ab200615 has not yet been referenced specifically in any publications.

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