Recombinant HRP Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab209022)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR12285] to mtTFA - Mitochondrial Marker
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker
See all mtTFA primary antibodies -
Description
HRP Rabbit monoclonal [EPR12285] to mtTFA - Mitochondrial Marker -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, MCF7 and HEK293 whole cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12285 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab209022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 26 kDa (predicted molecular weight: 29 kDa).
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Notes |
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WB
1/5000. Detects a band of approximately 26 kDa (predicted molecular weight: 29 kDa). |
Target
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Function
Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M. -
Sequence similarities
Contains 2 HMG box DNA-binding domains. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 7019 Human
- Omim: 600438 Human
- SwissProt: Q00059 Human
- Unigene: 642966 Human
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Alternative names
- anscription factor 6-like 1 antibody
- Mitochondrial transcription factor 1 antibody
- mitochondrial transcription factor A antibody
see all
Images
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All lanes : HRP Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab209022) at 1/5000 dilution
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209022 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab209022 has not yet been referenced specifically in any publications.