Recombinant HRP Anti-Mucin 5AC antibody [EPR16904] (ab218713)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Mucin 5AC antibody [EPR16904] (ab218713)](https://www.abcam.com/ps/products/218/ab218713/Images/ab218713-286950-IHCAP00357431.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Mucin 5AC antibody [EPR16904] (ab218713)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR16904] to Mucin 5AC
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-Mucin 5AC antibody [EPR16904]
See all Mucin 5AC primary antibodies -
Description
HRP Rabbit monoclonal [EPR16904] to Mucin 5AC -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Mucin 5AC aa 2300-2500. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P98088 -
Positive control
- IHC-P: normal human stomach tissue sections
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EPR16904 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
|
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system. -
Tissue specificity
Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum. -
Sequence similarities
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 3 TIL (trypsin inhibitory-like) domains.
Contains 4 VWFC domains.
Contains 4 VWFD domains. -
Domain
The cysteine residues in the Cys-rich subdomain repeats are not involved in disulfide bonding. -
Post-translational
modificationsC-, O- and N-glycosylated. O-glycosylated on the Thr-/Ser-rich tandem repeats. C-mannosylation in the Cys-rich subdomains may be required for proper folding of these regions and for export from the endoplasmic reticulum during biosynthesis.
Proteolytic cleavage in the C-terminal is initiated early in the secretory pathway and does not involve a serine protease. The extent of cleavage is increased in the acidic parts of the secretory pathway. Cleavage generates a reactive group which could link the protein to a primary amide. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 4586 Human
- Omim: 158373 Human
- SwissProt: P98088 Human
- Unigene: 534332 Human
- Unigene: 558950 Human
- Unigene: 703588 Human
- Unigene: 703728 Human
- Unigene: 721515 Human
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Alternative names
- Apomucin antibody
- Apomucin Major Airway Glycoprotein antibody
- Gastric mucin antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Mucin 5AC antibody [EPR16904] (ab218713)
IHC image of Mucin 5AC staining in a section of formalin-fixed paraffin-embedded normal human stomach*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab218713, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![HRP Anti-Mucin 5AC antibody [EPR16904] (ab218713)](https://www.abcam.com/ps/products/218/ab218713/Images/ab218713-2-benefits-of-recombinant-antibodies.png)
HRP Anti-Mucin 5AC antibody [EPR16904] (ab218713)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab218713 has not yet been referenced specifically in any publications.