Recombinant HRP Anti-Mucin 5AC antibody [EPR16904] (ab218713)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Mucin 5AC antibody [EPR16904] (ab218713)](https://www.abcam.com/ps/products/218/ab218713/Images/ab218713-286950-IHCAP00357431.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Mucin 5AC antibody [EPR16904] (ab218713)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR16904] to Mucin 5AC
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-Mucin 5AC antibody [EPR16904]
See all Mucin 5AC primary antibodies -
Description
HRP Rabbit monoclonal [EPR16904] to Mucin 5AC -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Mucin 5AC aa 2300-2500. The exact sequence is proprietary.
Database link: P98088 -
Positive control
- IHC-P: normal human stomach tissue sections
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16904 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab218713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
Mucins are high molecular weight glycoproteins with 80% carbohydrates and 20% core protein. Gastric Mucin 5AC antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden Mucin 5AC antigen. -
Cellular localization
Secreted; Cytoplasmic and cell surface -
Database links
- Entrez Gene: 4586 Human
- Omim: 158373 Human
- SwissProt: P98088 Human
- Unigene: 534332 Human
- Unigene: 558950 Human
- Unigene: 703588 Human
- Unigene: 703728 Human
- Unigene: 721515 Human
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Alternative names
- Apomucin antibody
- Apomucin Major Airway Glycoprotein antibody
- Gastric mucin antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Mucin 5AC antibody [EPR16904] (ab218713)
IHC image of Mucin 5AC staining in a section of formalin-fixed paraffin-embedded normal human stomach*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab218713, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![HRP Anti-Mucin 5AC antibody [EPR16904] (ab218713)](https://www.abcam.com/ps/products/218/ab218713/Images/ab218713-2-benefits-of-recombinant-antibodies.png)
HRP Anti-Mucin 5AC antibody [EPR16904] (ab218713)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab218713 has not yet been referenced specifically in any publications.
