HRP Anti-Myc tag antibody [9E10] (ab62928)
Key features and details
- HRP Mouse monoclonal [9E10] to Myc tag
- Suitable for: ELISA, WB
- Conjugation: HRP
- Isotype: IgG1
Overview
-
Product name
HRP Anti-Myc tag antibody [9E10]
See all Myc tag primary antibodies -
Description
HRP Mouse monoclonal [9E10] to Myc tag -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: ELISA, WBmore details -
Immunogen
Synthetic peptide corresponding to Human Myc tag aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
-
Positive control
- Purchase matching WB positive control:Recombinant human c-Myc protein (Active)
- This antibody gave a positive signal in E.coli Whole Cell Lysate (positive control) expressing 11 different epitope tags (ab5395).
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol
contains 0.4M Arginine -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
9E10 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
- DyLight® 650 Anti-Myc tag antibody [9E10] (ab117487)
- DyLight® 488 Anti-Myc tag antibody [9E10] (ab117499)
- DyLight® 550 Anti-Myc tag antibody [9E10] (ab117511)
- FITC Anti-Myc tag antibody [9E10] (ab117599)
- Biotin Anti-Myc tag antibody [9E10] - C-terminal (ab197139)
- Alexa Fluor® 488 Anti-Myc tag antibody [9E10] (ab202008)
- Anti-Myc tag antibody [9E10] (ab206486)
- Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)
- Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)
- APC Anti-Myc tag antibody [9E10] (ab223896)
- Anti-Myc tag antibody [9E10] - BSA and Azide free (ab264603)
- Anti-Myc tag antibody [9E10] (ab32)
- PE Anti-Myc tag antibody [9E10] (ab72468)
- SureLight® APC Anti-Myc tag antibody [9E10] (ab72580)
- Biotin Anti-Myc tag antibody [9E10] (ab81658)
-
Assay kits
-
Related Products
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Applications
Our Abpromise guarantee covers the use of ab62928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA | 1/100 - 1/500. | |
WB | 1/10000 - 1/50000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
Target
-
Relevance
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
Cellular localization
Nuclear -
Alternative names
- c-myc tag antibody
- Myc Epitope Tag antibody
Images
-
HRP Anti-Myc tag antibody [9E10] (ab62928) at 1/10000 dilution +
E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 41 kDa
Protocols
References (20)
ab62928 has been referenced in 20 publications.
- Chung Y et al. Auxin Response Factors promote organogenesis by chromatin-mediated repression of the pluripotency gene SHOOTMERISTEMLESS. Nat Commun 10:886 (2019). PubMed: 30792395
- Tian J et al. Feline Herpesvirus 1 US3 Blocks the Type I Interferon Signal Pathway by Targeting Interferon Regulatory Factor 3 Dimerization in a Kinase-Independent Manner. J Virol 92:N/A (2018). PubMed: 29618645
- Rivera-Reyes R et al. Proteomic analysis identifies transcriptional cofactors and homeobox transcription factors as TBX18 binding proteins. PLoS One 13:e0200964 (2018). PubMed: 30071041
- Takizawa M et al. Mechanistic basis for the recognition of laminin-511 by a6ß1 integrin. Sci Adv 3:e1701497 (2017). PubMed: 28879238
- Kunde SA et al. Protein kinase C regulates AMPA receptor auxiliary protein Shisa9/CKAMP44 through interactions with neuronal scaffold PICK1. FEBS Open Bio 7:1234-1245 (2017). PubMed: 28904854