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  1. Link

    hrp-myc-tag-antibody-9e10-ab62928.pdf

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Tags & Cell Markers Epitope Tags Myc Tag
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HRP Anti-Myc tag antibody [9E10] (ab62928)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (20)

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Abpromise

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Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)

    Key features and details

    • HRP Mouse monoclonal [9E10] to Myc tag
    • Suitable for: ELISA, WB
    • Conjugation: HRP
    • Isotype: IgG1

    Overview

    • Product name

      HRP Anti-Myc tag antibody [9E10]
      See all Myc tag primary antibodies
    • Description

      HRP Mouse monoclonal [9E10] to Myc tag
    • Host species

      Mouse
    • Conjugation

      HRP
    • Tested applications

      Suitable for: ELISA, WBmore details
    • Immunogen

      Synthetic peptide corresponding to Human Myc tag aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.

    • Positive control

      • Purchase matching WB positive control:Recombinant human c-Myc protein (Active)
      • This antibody gave a positive signal in E.coli Whole Cell Lysate (positive control) expressing 11 different epitope tags (ab5395).
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
    • Storage buffer

      pH: 7.40
      Preservative: 0.1% 10% Proclin 300 Solution
      Constituents: PBS, 1% BSA, 30% Glycerol

      contains 0.4M Arginine
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Monoclonal
    • Clone number

      9E10
    • Myeloma

      Sp2/0
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Tags & Cell Markers
      • Epitope Tags
      • Myc Tag
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • HLH / Leucine Zipper
      • HLH / Leucine Zipper
      • Stem Cells
      • Signaling Pathways
      • TGF beta
      • Nuclear
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Transcription Factors
      • Cancer
      • Cell cycle
      • Cell differentiation
      • Cancer
      • Oncoproteins/suppressors
      • Oncoproteins
      • Transcription factors
      • Cancer
      • Tumor biomarkers
      • Oncoproteins

    Associated products

    • Alternative Versions

      • DyLight® 650 Anti-Myc tag antibody [9E10] (ab117487)
      • DyLight® 488 Anti-Myc tag antibody [9E10] (ab117499)
      • DyLight® 550 Anti-Myc tag antibody [9E10] (ab117511)
      • FITC Anti-Myc tag antibody [9E10] (ab117599)
      • Biotin Anti-Myc tag antibody [9E10] - C-terminal (ab197139)
      • Alexa Fluor® 488 Anti-Myc tag antibody [9E10] (ab202008)
      • Anti-Myc tag antibody [9E10] (ab206486)
      • Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)
      • Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)
      • APC Anti-Myc tag antibody [9E10] (ab223896)
      • Anti-Myc tag antibody [9E10] - BSA and Azide free (ab264603)
      • Anti-Myc tag antibody [9E10] (ab32)
      • PE Anti-Myc tag antibody [9E10] (ab72468)
      • SureLight® APC Anti-Myc tag antibody [9E10] (ab72580)
      • Biotin Anti-Myc tag antibody [9E10] (ab81658)
    • Assay kits

      • c-Myc Transcription Factor Assay Kit (Colorimetric) (ab207200)
    • Related Products

      • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
      • TMB ELISA Substrate (High Sensitivity) (ab171523)
      • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
      • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
      • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
      • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
      • 450 nm Stop Solution for TMB Substrate (ab171529)
      • 650 nm Stop Solution for TMB Substrate (ab171531)
      • Immunoassay Blocking Buffer (ab171534)
      • Immunoassay Blocking (BSA Free) (ab171535)

    Applications

    Our Abpromise guarantee covers the use of ab62928 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA 1/100 - 1/500.
    WB 1/10000 - 1/50000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

    Target

    • Relevance

      Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
    • Cellular localization

      Nuclear
    • Alternative names

      • c-myc tag antibody
      • Myc Epitope Tag antibody

    Images

    • Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)
      Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)
      HRP Anti-Myc tag antibody [9E10] (ab62928) at 1/10000 dilution + E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg

      Performed under reducing conditions.

      Predicted band size: 41 kDa
      Observed band size: 41 kDa

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (20)

    Publishing research using ab62928? Please let us know so that we can cite the reference in this datasheet.

    ab62928 has been referenced in 20 publications.

    • Chung Y  et al. Auxin Response Factors promote organogenesis by chromatin-mediated repression of the pluripotency gene SHOOTMERISTEMLESS. Nat Commun 10:886 (2019). PubMed: 30792395
    • Tian J  et al. Feline Herpesvirus 1 US3 Blocks the Type I Interferon Signal Pathway by Targeting Interferon Regulatory Factor 3 Dimerization in a Kinase-Independent Manner. J Virol 92:N/A (2018). PubMed: 29618645
    • Rivera-Reyes R  et al. Proteomic analysis identifies transcriptional cofactors and homeobox transcription factors as TBX18 binding proteins. PLoS One 13:e0200964 (2018). PubMed: 30071041
    • Takizawa M  et al. Mechanistic basis for the recognition of laminin-511 by a6ß1 integrin. Sci Adv 3:e1701497 (2017). PubMed: 28879238
    • Kunde SA  et al. Protein kinase C regulates AMPA receptor auxiliary protein Shisa9/CKAMP44 through interactions with neuronal scaffold PICK1. FEBS Open Bio 7:1234-1245 (2017). PubMed: 28904854
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    Do you have information on the sensitivity of your anti-Myc-tag antibody? What is the minimal amount of protein I need to load onto a gel to detect my Myc-tagged fusion protein?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 29 2013

    Answer

    The laboratory confirmed that they tested 0.1ug E. coli with myc-tag in WB and they obtained a good signal at 1 min exposure. However, they did not test the limits of ab62928 products sensitivity, but they would expect it to be able to detect smaller quantities than they have used.


    Read More

    Abcam Scientific Support

    Answered on Oct 29 2013

    Western blot abreview for Anti-c-Myc antibody [9E10] (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Escherichia coli Recombinant protein (BL21(DE3))
    Loading amount
    0.5 µg
    Specification
    BL21(DE3)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 30 2010

    Western blot abreview for Anti-c-Myc antibody [9E10] (HRP)

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T cells)
    Loading amount
    20 µg
    Specification
    293T cells
    Gel Running Conditions
    Reduced Denaturing (Invitrogen NUPAGE Bis-Tris Gel 4-12% Gradient Gel MES Buffer)
    Blocking step
    Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 25 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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