HRP Anti-Myc tag antibody [9E10] (ab62928)

Reviews (2)Q&A (1)References (20)
Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)

    Key features and details

    • HRP Mouse monoclonal [9E10] to Myc tag
    • Suitable for: ELISA, WB
    • Reacts with: Recombinant fragment
    • Conjugation: HRP
    • Isotype: IgG1

    Overview

    • Product name

      HRP Anti-Myc tag antibody [9E10]
      See all Myc tag primary antibodies

    • Description

      HRP Mouse monoclonal [9E10] to Myc tag

    • Host species

      Mouse

    • Conjugation

      HRP

    • Tested applications

      Suitable for: ELISA, WBmore details

    • Species reactivity

      Reacts with: Recombinant fragment

    • Immunogen

      Synthetic peptide corresponding to Human Myc tag aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.

    • Positive control

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab62928 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA
    1/100 - 1/500.
    WB (2)
    1/10000 - 1/50000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
    Notes
    ELISA
    1/100 - 1/500.
    WB
    1/10000 - 1/50000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

    Target

    • Relevance

      Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

    • Cellular localization

      Nuclear

    • Alternative names

      • c-myc tag antibody
      • Myc Epitope Tag antibody

    Images

    • Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)
      Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)
      HRP Anti-Myc tag antibody [9E10] (ab62928) at 1/10000 dilution + E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg

      Performed under reducing conditions.

      Predicted band size: 41 kDa
      Observed band size: 41 kDa

    References (20)

    Publishing research using ab62928? Please let us know so that we can cite the reference in this datasheet.

    ab62928 has been referenced in 20 publications.

    • Chung Y  et al. Auxin Response Factors promote organogenesis by chromatin-mediated repression of the pluripotency gene SHOOTMERISTEMLESS. Nat Commun 10:886 (2019). PubMed: 30792395
    • Tian J  et al. Feline Herpesvirus 1 US3 Blocks the Type I Interferon Signal Pathway by Targeting Interferon Regulatory Factor 3 Dimerization in a Kinase-Independent Manner. J Virol 92:N/A (2018). PubMed: 29618645
    • Rivera-Reyes R  et al. Proteomic analysis identifies transcriptional cofactors and homeobox transcription factors as TBX18 binding proteins. PLoS One 13:e0200964 (2018). PubMed: 30071041
    • Takizawa M  et al. Mechanistic basis for the recognition of laminin-511 by a6ß1 integrin. Sci Adv 3:e1701497 (2017). PubMed: 28879238
    • Kunde SA  et al. Protein kinase C regulates AMPA receptor auxiliary protein Shisa9/CKAMP44 through interactions with neuronal scaffold PICK1. FEBS Open Bio 7:1234-1245 (2017). PubMed: 28904854
    View all Publications for this product

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-c-Myc antibody [9E10] (HRP)

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Escherichia coli Recombinant protein (BL21(DE3))
    Loading amount
    0.5 µg
    Specification
    BL21(DE3)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 30 2010

    Western blot abreview for Anti-c-Myc antibody [9E10] (HRP)

     Excellent
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T cells)
    Loading amount
    20 µg
    Specification
    293T cells
    Gel Running Conditions
    Reduced Denaturing (Invitrogen NUPAGE Bis-Tris Gel 4-12% Gradient Gel MES Buffer)
    Blocking step
    Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 25 2010

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