HRP Anti-Myc tag antibody (ab1261)
Key features and details
- HRP Goat polyclonal to Myc tag
- Suitable for: ELISA, WB, ICC
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-Myc tag antibody
See all Myc tag primary antibodies -
Description
HRP Goat polyclonal to Myc tag -
Host species
Goat -
Conjugation
HRP -
Tested applications
Suitable for: ELISA, WB, ICCmore details -
Immunogen
Full length native protein
EQKLISEEDL
(c-myc) (purified): conjugated to KLH. -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinitypurified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Immunizing Peptide (Blocking)
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Applications
Our Abpromise guarantee covers the use of ab1261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/10000 - 1/100000. | |
| WB | 1/1000 - 1/30000. Can be blocked with Human c-Myc peptide (ab13837). | |
| ICC | 1/200 - 1/500. |
Target
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Relevance
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
Cellular localization
Nuclear -
Alternative names
- c-myc tag antibody
- Myc Epitope Tag antibody
Protocols
References (7)
ab1261 has been referenced in 7 publications.
- Zhao Q et al. Rational library design by functional CDR resampling. N Biotechnol 45:89-97 (2018). PubMed: 29242049
- Neira-Peña T et al. Perinatal asphyxia leads to PARP-1 overactivity, p65 translocation, IL-1ß and TNF-a overexpression, and apoptotic-like cell death in mesencephalon of neonatal rats: prevention by systemic neonatal nicotinamide administration. Neurotox Res 27:453-65 (2015). WB . PubMed: 25835215
- Iorio-Morin C et al. Thromboxane A2 modulates cisplatin-induced apoptosis through a Siva1-dependent mechanism. Cell Death Differ 19:1347-57 (2012). PubMed: 22343716
- Aavikko M et al. Loss of SUFU function in familial multiple meningioma. Am J Hum Genet 91:520-6 (2012). PubMed: 22958902
- Zhang-James Y et al. SLC9A9 mutations, gene expression, and protein-protein interactions in rat models of attention-deficit/hyperactivity disorder. Am J Med Genet B Neuropsychiatr Genet 156B:835-43 (2011). PubMed: 21858920
- Cartier A et al. WDR36 acts as a scaffold protein tethering a G-protein-coupled receptor, Gaq and phospholipase Cß in a signalling complex. J Cell Sci 124:3292-304 (2011). WB . PubMed: 21940795
- Millar DS et al. Characterisation of a functional intronic polymorphism in the human growth hormone (GH1) gene. Hum Genomics 4:289-301 (2010). PubMed: 20650818
