Key features and details
- HRP Rabbit polyclonal to Myc tag
- Suitable for: ELISA, WB, ICC
- Conjugation: HRP
- Isotype: IgG
Product nameHRP Anti-Myc tag antibody
See all Myc tag primary antibodies
DescriptionHRP Rabbit polyclonal to Myc tag
SpecificityIn a direct ELISA against 1 ug of C-Myc peptide coupled to BSA, conjugate diluted to 1/20,000 produced a 1.0 OD in a 15 minute reaction with TMB as substrate.
Tested applicationsSuitable for: ELISA, WB, ICCmore details
Synthetic peptide: EQKLISEEDL conjugated to KLH.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.00
Preservative: 0.002% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
Concentration information loading...
Purification notesAntibodies were immunoaffinity purified using the peptide conjugated to a solid phase support, and conjugated to horseradish peroxidase.
Immunizing Peptide (Blocking)
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab1326 in the following tested applications.
|ELISA||1/5000 - 1/20000.|
|WB||1/1000 - 1/5000. Can be blocked with Human c-Myc peptide (ab13837).|
|ICC||1/200 - 1/500.|
RelevanceEpitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
- c-myc tag antibody
- Myc Epitope Tag antibody
ab1326 has been referenced in 15 publications.
- Lee S et al. Absence of Cytosolic 2-Cys Prx Subtypes I and II Exacerbates TNF-a-Induced Apoptosis via Different Routes. Cell Rep 26:2194-2211.e6 (2019). PubMed: 30784599
- Wild P et al. Network Rewiring of Homologous Recombination Enzymes during Mitotic Proliferation and Meiosis. Mol Cell 75:859-874.e4 (2019). PubMed: 31351878
- Xing J et al. TRIM29 Negatively Regulates the Type I IFN Production in Response to RNA Virus. J Immunol 201:183-192 (2018). PubMed: 29769269
- Compte M et al. A tumor-targeted trimeric 4-1BB-agonistic antibody induces potent anti-tumor immunity without systemic toxicity. Nat Commun 9:4809 (2018). PubMed: 30442944
- Kim JH et al. WNK1 kinase is essential for insulin-stimulated GLUT4 trafficking in skeletal muscle. FEBS Open Bio 8:1866-1874 (2018). PubMed: 30410865