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  1. Link

    hrp-mycobacterium-tuberculosis-antibody-ab21189.pdf

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Microbiology Organism Bacteria Gram positive bacteria Mycobacterium
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HRP Anti-Mycobacterium tuberculosis antibody (ab21189)

  • Datasheet
  • SDS
Submit a review Q&A (1)

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Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

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Key features and details

  • HRP Rabbit polyclonal to Mycobacterium tuberculosis
  • Suitable for: IHC-P
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG

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Overview

  • Product name

    HRP Anti-Mycobacterium tuberculosis antibody
    See all Mycobacterium tuberculosis primary antibodies
  • Description

    HRP Rabbit polyclonal to Mycobacterium tuberculosis
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Tissue, cells or virus corresponding to Mycobacterium tuberculosis. Mycobacterium tuberculosis purified protein derivative

  • General notes

    IgG fraction covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while reserving maximum enzyme activity. Free enzyme is absent. Estimated Molar HRP: IgG substitution is 2-3

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.002% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Bacteria
    • Gram positive bacteria
    • Mycobacterium

Associated products

  • Related Products

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 450 nm Stop Solution for TMB Substrate (ab171529)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab21189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent dilution.
Notes
IHC-P
Use at an assay dependent dilution.

Target

  • Relevance

    Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure; most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.
  • Alternative names

    • M. tuberculosis antibody

Protocols

  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab21189? Please let us know so that we can cite the reference in this datasheet.

ab21189 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

I am looking for antimycobacterium antibodies for immunohistochemistry and got interested with your products ab20027 and also ab21189 and ab905. Concerning first two products I have a question what dilution is recommended for them? What does "use at an assay dependent dilution" means? Secondly, in the ab21189 datasheet nothing is said about antigen retrieval. Does it mean that no retrieval is required the same as for ab20027?

Read More

Abcam community

Verified customer

Asked on Jan 22 2007

Answer

As a follow up to my previous message, I can now confirm that ab21189 does not require antigen retrieval. I hope this information helps but please don't hesitate to contact me should you require any additional assistance.

Read More

Abcam Scientific Support

Answered on Jan 23 2007

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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