RecombinantRabMAb

Recombinant HRP Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195524)

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Western blot - HRP Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195524)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195524)
  • HRP Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195524)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [UOTR1B492] to NADPH oxidase 4
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-NADPH oxidase 4 antibody [UOTR1B492]
    See all NADPH oxidase 4 primary antibodies

  • Description

    HRP Rabbit monoclonal [UOTR1B492] to NADPH oxidase 4

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human NADPH oxidase 4 aa 500 to the C-terminus.
    (Peptide available as ab179799)

  • Positive control

    • WB: HEK293 (Human embryonic kidney cell line) Whole Cell lysates. IHC-P: FFPE human normal kidney tissue sections.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab195524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 67 kDa).Can be blocked with NADPH oxidase 4 peptide (ab179799).

Target

  • Function

    Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4 displays an increased activity. Isoform 5 and isoform 6 display reduced activity.

  • Tissue specificity

    Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells.

  • Sequence similarities

    Contains 1 FAD-binding FR-type domain.
    Contains 1 ferric oxidoreductase domain.

  • Developmental stage

    Expressed in fetal kidney and fetal liver.

  • Post-translational
    modifications

    Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function.

  • Cellular localization

    Endoplasmic reticulum membrane. Cell membrane. Cell junction > focal adhesion. Nucleus. May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Kidney oxidase 1 antibody
      • Kidney oxidase-1 antibody
      • Kidney superoxide producing NADPH oxidase antibody
      • Kidney superoxide-producing NADPH oxidase antibody
      • KOX 1 antibody
      • KOX antibody
      • Kox-1 antibody
      • KOX1 antibody
      • NADPH antibody
      • NADPH oxidase 4 antibody
      • Nox4 antibody
      • NOX4_HUMAN antibody
      • Renal NAD(P)H oxidase antibody
      • Renal NAD(P)H-oxidase antibody
      • RENOX antibody
      see all

    Images

    • Western blot - HRP Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195524)
      Western blot - HRP Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195524)
      HRP Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195524) at 1/1000 dilution + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 67 kDa
      Observed band size: 63 kDa
      why is the actual band size different from the predicted?


      Exposure time: 90 seconds


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab195524 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195524)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195524)

      IHC image of NADPH oxidase 4 staining in a section of formalin-fixed paraffin-embedded human normal kidney*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab195524 at a dilution of 1/500. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

    • HRP Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195524)
      HRP Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195524)

    References (3)

    Publishing research using ab195524? Please let us know so that we can cite the reference in this datasheet.

    ab195524 has been referenced in 3 publications.

    • Kim HJ  et al. Prevention of Oxidative Stress-Induced Pancreatic Beta Cell Damage by Broussonetia Kazinoki Siebold Fruit Extract Via the ERK-Nox4 Pathway. Antioxidants (Basel) 9:N/A (2020). PubMed: 32397640
    • Yisireyili M  et al. Chronic restraint stress induces esophageal fibrosis with enhanced oxidative stress in a murine model. Exp Ther Med 18:1375-1383 (2019). PubMed: 31316626
    • Han H  et al. Dexmedetomidine improves cardiac function and protects against maladaptive remodeling following myocardial infarction. Mol Med Rep 20:5183-5189 (2019). PubMed: 31661145

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