Recombinant HRP Anti-NDUFV2 antibody [EPR15351(B)] (ab209040)
![Western blot - HRP Anti-NDUFV2 antibody [EPR15351(B)] (ab209040)](https://www.abcam.com/ps/products/209/ab209040/Images/ab209040-270881-WBab2090401.jpg "Western blot - HRP Anti-NDUFV2 antibody [EPR15351(B)] (ab209040)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR15351(B)] to NDUFV2
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-NDUFV2 antibody [EPR15351(B)]
See all NDUFV2 primary antibodies -
Description
HRP Rabbit monoclonal [EPR15351(B)] to NDUFV2 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NDUFV2 aa 200 to the C-terminus. The exact sequence is proprietary.
Database link: P19404 -
Positive control
- WB: Human Fetal Heart tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15351(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab209040 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 24 kDa (predicted molecular weight: 27 kDa). |
Target
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Function
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
Sequence similarities
Belongs to the complex I 24 kDa subunit family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 4729 Human
- Entrez Gene: 72900 Mouse
- Entrez Gene: 81728 Rat
- Omim: 600532 Human
- SwissProt: P19404 Human
- SwissProt: Q9D6J6 Mouse
- SwissProt: P19234 Rat
- Unigene: 464572 Human
see all -
Alternative names
- 24kDa subunit of Complex I antibody
- CI-24k antibody
- complex I 24kDa subunit antibody
see all
Images
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Western blot - HRP Anti-NDUFV2 antibody [EPR15351(B)] (ab209040)HRP Anti-NDUFV2 antibody [EPR15351(B)] (ab209040) at 1/5000 dilution + Heart (Human) Tissue Lysate - fetal normal tissue at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209040 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-NDUFV2 antibody [EPR15351(B)] (ab209040)](https://www.abcam.com/ps/products/209/ab209040/Images/ab209040-2-benefits-of-recombinant-antibodies.png)
HRP Anti-NDUFV2 antibody [EPR15351(B)] (ab209040)
References (0)
ab209040 has not yet been referenced specifically in any publications.
