HRP Anti-Nitrotyrosine antibody (ab27648)
Key features and details
- HRP Goat polyclonal to Nitrotyrosine
- Suitable for: ELISA, WB
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-Nitrotyrosine antibody
See all Nitrotyrosine primary antibodies -
Description
HRP Goat polyclonal to Nitrotyrosine -
Host species
Goat -
Conjugation
HRP -
Tested applications
Suitable for: ELISA, WBmore details -
Immunogen
Chemical/ Small Molecule (nitrotyrosine modified protein)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.134% PBS, 0.58% Sodium chloride -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified by nitrated protein-Sepharose™ affinity column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Target
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Relevance
The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab27648 has been referenced in 1 publication.
- Tang Z et al. Sensitive immunoassays of nitrated fibrinogen in human biofluids. Talanta 81:1662-9 (2010). ELISA . PubMed: 20441955