Key features and details
- HRP Mouse monoclonal [8B4BB10] to NNT
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Product nameHRP Anti-NNT antibody [8B4BB10]
See all NNT primary antibodies
DescriptionHRP Mouse monoclonal [8B4BB10] to NNT
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Tissue, cells or virus corresponding to Human NNT.
- WB: HepG2 whole cell lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
Purification notesProduced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE.
Light chain typekappa
Our Abpromise guarantee covers the use of ab198312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa).|
FunctionThe transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.
Sequence similaritiesIn the N-terminal section; belongs to the AlaDH/PNT family.
In the C-terminal section; belongs to the PNT beta subunit family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- GCCD4 antibody
- mitochondrial antibody
- NAD(P) transhydrogenase antibody
HRP Anti-NNT antibody [8B4BB10] (ab198312) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 114 kDa
Observed band size: 114 kDa
Exposure time: 8 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab198312 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab198312 has not yet been referenced specifically in any publications.