RecombinantRabMAb

Recombinant HRP Anti-p73 antibody [EP436Y] (ab197040)

Submit a review Submit a question References (1)
Western blot - HRP Anti-p73 antibody [EP436Y] (ab197040)
  • HRP Anti-p73 antibody [EP436Y] (ab197040)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EP436Y] to p73
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-p73 antibody [EP436Y]
    See all p73 primary antibodies

  • Description

    HRP Rabbit monoclonal [EP436Y] to p73

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide within Human p73 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, Jurkat and NIH 3T3 whole cell lysates.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab197040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/3000. Detects a band of approximately 63 kDa (predicted molecular weight: 70 kDa).

Target

  • Function

    Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein.

  • Tissue specificity

    Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue.

  • Sequence similarities

    Belongs to the p53 family.
    Contains 1 SAM (sterile alpha motif) domain.

  • Domain

    Possesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL tyrosine kinase SH3 domain.
    The WW-binding motif mediates interaction with WWOX.

  • Post-translational
    modifications

    Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity.
    Higher levels of phosphorylation seen in the brain from patients with Huntington disease.
    Ubiquitinated; leading to its degradation by the proteasome.

  • Cellular localization

    Nucleus. Accumulates in the nucleus in response to DNA damage.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • p53 like transcription factor antibody
    • p53 related protein antibody
    • p53-like transcription factor antibody
    • p53-related protein antibody
    • p73 antibody
    • P73_HUMAN antibody
    • TP73 antibody
    • Tumor protein p73 antibody
    see all

Images

  • Western blot - HRP Anti-p73 antibody [EP436Y] (ab197040)
    Western blot - HRP Anti-p73 antibody [EP436Y] (ab197040)
    All lanes : HRP Anti-p73 antibody [EP436Y] (ab197040) at 1/3000 dilution

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 70 kDa
    Observed band size: 63 kDa
    why is the actual band size different from the predicted?


    Exposure time: 16 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197040 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • HRP Anti-p73 antibody [EP436Y] (ab197040)
    HRP Anti-p73 antibody [EP436Y] (ab197040)

References (1)

Publishing research using ab197040? Please let us know so that we can cite the reference in this datasheet.

ab197040 has been referenced in 1 publication.

  • Diaz-Flores E  et al. Bcl-2 Is a Therapeutic Target for Hypodiploid B-Lineage Acute Lymphoblastic Leukemia. Cancer Res 79:2339-2351 (2019). PubMed: 30862722

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab197040.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com