RecombinantRabMAb

Recombinant HRP Anti-PABPN1 antibody [EP3000Y] (ab207515)

Submit a review Submit a question
Western blot - HRP Anti-PABPN1 antibody [EP3000Y] (ab207515)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-PABPN1 antibody [EP3000Y] (ab207515)
  • HRP Anti-PABPN1 antibody [EP3000Y] (ab207515)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EP3000Y] to PABPN1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-PABPN1 antibody [EP3000Y]
    See all PABPN1 primary antibodies

  • Description

    HRP Rabbit monoclonal [EP3000Y] to PABPN1

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human PABPN1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q86U42

  • Positive control

    • WB: RAW 264.7 whole cell lysate. IHC-P: FFPE normal human testis tissue sections.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab207515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation.

  • Tissue specificity

    Ubiquitous.

  • Involvement in disease

    Defects in PABPN1 are the cause of oculopharyngeal muscular dystrophy (OPMD) [MIM:164300]. OPMD is a form of late-onset slowly progressive myopathy characterized by eyelid ptosis, dysphagia and, sometimes by other cranial and limb-muscle involvement.

  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.

  • Domain

    The RRM domain is essential for specific adenine bases recognition in the poly(A) tail but not sufficient for poly(A) binding.

  • Post-translational
    modifications

    Arginine dimethylation is asymmetric and involves PRMT1 and PRMT3. It does not influence the RNA binding properties.

  • Cellular localization

    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between the nucleus and the cytoplasm but predominantly found in the nucleus. Its nuclear import may involve the nucleocytoplasmic transport receptor transportin and a RAN-GTP-sensitive import mechanism. Is exported to the cytoplasm by a carrier-mediated pathway that is independent of mRNA traffic. Nucleus; nuclear speckle. Colocalizes with SKIP and poly(A) RNA in nuclear speckles.
  • Information by UniProt

  • Database links

    • Alternative names

      • Nuclear poly(A)-binding protein 1 antibody
      • OPMD antibody
      • PAB2 antibody
      • PABII antibody
      • PABP 2 antibody
      • pABP-2 antibody
      • PABP2 antibody
      • PABP2_HUMAN antibody
      • PABPII antibody
      • Pabpn1 antibody
      • poly(A) binding protein nuclear 1 antibody
      • Poly(A)-binding protein 2 antibody
      • Poly(A)-binding protein II antibody
      • PolyA binding protein II antibody
      • Polyadenylate-binding nuclear protein 1 antibody
      • Polyadenylate-binding protein 2 antibody
      see all

    Images

    • Western blot - HRP Anti-PABPN1 antibody [EP3000Y] (ab207515)
      Western blot - HRP Anti-PABPN1 antibody [EP3000Y] (ab207515)
      HRP Anti-PABPN1 antibody [EP3000Y] (ab207515) at 1/5000 dilution + RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate at 10 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 33 kDa
      Observed band size: 44 kDa
      why is the actual band size different from the predicted?


      Exposure time: 90 seconds


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab207515 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-PABPN1 antibody [EP3000Y] (ab207515)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-PABPN1 antibody [EP3000Y] (ab207515)

      IHC image of PABPN1 staining in a section of formalin-fixed paraffin-embedded normal human testis tissue*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins.  The section was then incubated with ab207515, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • HRP Anti-PABPN1 antibody [EP3000Y] (ab207515)
      HRP Anti-PABPN1 antibody [EP3000Y] (ab207515)

    References (0)

    Publishing research using ab207515? Please let us know so that we can cite the reference in this datasheet.

    ab207515 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab207515.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com