Key features and details
- HRP Mouse monoclonal [mAbcam22744] to pan Cadherin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
- Isotype: IgG1
Product nameHRP Anti-pan Cadherin antibody [mAbcam22744]
See all pan Cadherin primary antibodies
DescriptionHRP Mouse monoclonal [mAbcam22744] to pan Cadherin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Dog, Xenopus laevis, Zebrafish
Synthetic peptide corresponding to Human pan Cadherin aa 850 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
- WB: Rat, mouse and human heart tissue lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
Light chain typekappa
Our Abpromise guarantee covers the use of ab197725 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 125 kDa (predicted molecular weight: 125 kDa).|
RelevanceCadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis.
- Cadherin antibody
- CDH3 antibody
- CDHP antibody
All lanes : HRP Anti-pan Cadherin antibody [mAbcam22744] (ab197725) at 1/5000 dilution
Lane 1 : Heart (Rat) Tissue Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : Heart (Human) Tissue Lysate - adult normal tissue
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 125 kDa
Observed band size: 125 kDa
Exposure time: 1 minute
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197725 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab197725 has not yet been referenced specifically in any publications.