HRP Anti-pan Cadherin antibody [mAbcam22744] (ab197725)
![Western blot - HRP Anti-pan Cadherin antibody [mAbcam22744] (ab197725)](https://www.abcam.com/ps/products/197/ab197725/Images/ab197725-238353-anti-pan-cadherin-antibody-mabcam22744-hrp-western-blot.jpg "Western blot - HRP Anti-pan Cadherin antibody [mAbcam22744] (ab197725)")
Key features and details
- HRP Mouse monoclonal [mAbcam22744] to pan Cadherin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-pan Cadherin antibody [mAbcam22744]
See all pan Cadherin primary antibodies -
Description
HRP Mouse monoclonal [mAbcam22744] to pan Cadherin -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Dog, Xenopus laevis, Zebrafish
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Immunogen
Synthetic peptide corresponding to Human pan Cadherin aa 850 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
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Positive control
- WB: Rat, mouse and human heart tissue lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
mAbcam22744 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Related Products
Applications
Our Abpromise guarantee covers the use of ab197725 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 125 kDa (predicted molecular weight: 125 kDa). |
Target
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Relevance
Cadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis. -
Database links
- Entrez Gene: 1001 Human
- Entrez Gene: 1002 Human
- Entrez Gene: 1003 Human
- Entrez Gene: 1004 Human
- Entrez Gene: 1005 Human
- Entrez Gene: 1006 Human
- Entrez Gene: 1008 Human
- Entrez Gene: 1009 Human
see all -
Alternative names
- Cadherin antibody
- CDH3 antibody
- CDHP antibody
see all
Images
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Western blot - HRP Anti-pan Cadherin antibody [mAbcam22744] (ab197725)All lanes : HRP Anti-pan Cadherin antibody [mAbcam22744] (ab197725) at 1/5000 dilution
Lane 1 : Heart (Rat) Tissue Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : Heart (Human) Tissue Lysate - adult normal tissue
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 125 kDa
Observed band size: 125 kDa
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197725 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
References (0)
ab197725 has not yet been referenced specifically in any publications.
