Recombinant HRP Anti-Pan Trk antibody [EPR17341] (ab209465)
![Western blot - HRP Anti-Pan Trk antibody [EPR17341] (ab209465)](https://www.abcam.com/ps/products/209/ab209465/Images/ab209465-265759-anti-pan-trk-antibody-epr17341-hrp-western-blot.jpg "Western blot - HRP Anti-Pan Trk antibody [EPR17341] (ab209465)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR17341] to Pan Trk
- Suitable for: WB
- Reacts with: Mouse, Rat
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-Pan Trk antibody [EPR17341]
See all Pan Trk primary antibodies -
Description
HRP Rabbit monoclonal [EPR17341] to Pan Trk -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse Brain and Rat Brain tissue lysates
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General notes
This is the Research Use Only (RUO) antibody of the clone that has been used in the in vitro diagnostic VENTANA pan-TRK (EPR17341) assay (an immunohistochemistry assay).
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17341 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab209465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000. Detects a band of approximately 119 kDa (predicted molecular weight: 92 kDa).
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| Notes |
|---|
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WB
1/5000. Detects a band of approximately 119 kDa (predicted molecular weight: 92 kDa). |
Target
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Relevance
Family of neurotrophic tyrosine kinase (NTRK1/2/3) genes which encode TrkA, TrkB and TrkC protein kinases. The three family members are activated by different neurotrophins: TrkA is activated by Nerve growth factor (NGF), TrkB by Brain-derived neurotrophic factor (BDNF) or neurotrophin-4 (NT-4) and TrkC by NT-3. Neurotrophin signalling activates cellular pathways involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. Localization TrkA: Cell membrane. Early endosome membrane. Late endosome membrane. Internalized to endosomes upon binding of NGF or NT-3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity). TrkB: Membrane. TrkC: Membrane. -
Database links
- Entrez Gene: 4914 Human
- Entrez Gene: 4915 Human
- Entrez Gene: 4916 Human
- Entrez Gene: 18211 Mouse
- Entrez Gene: 18212 Mouse
- Entrez Gene: 18213 Mouse
- Entrez Gene: 25054 Rat
- Entrez Gene: 29613 Rat
see all -
Alternative names
- BDNF/NT-3 growth factors receptor antibody
- EML4 antibody
- ETV6 antibody
see all
Images
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Western blot - HRP Anti-Pan Trk antibody [EPR17341] (ab209465)All lanes : HRP Anti-Pan Trk antibody [EPR17341] (ab209465) at 1/5000 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 119 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab209465 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-Pan Trk antibody [EPR17341] (ab209465)](https://www.abcam.com/ps/products/209/ab209465/Images/ab209465-2-benefits-of-recombinant-antibodies.png)
HRP Anti-Pan Trk antibody [EPR17341] (ab209465)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab209465 has not yet been referenced specifically in any publications.