Recombinant HRP Anti-PD-L1 antibody [EPR19759] (ab215349)
![Western blot - HRP Anti-PD-L1 antibody [EPR19759] (ab215349)](https://www.abcam.com/ps/products/215/ab215349/Images/ab215349-283943-anti-pd-l1-antibody-epr19759-hrp-western-blot.jpg "Western blot - HRP Anti-PD-L1 antibody [EPR19759] (ab215349)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR19759] to PD-L1
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-PD-L1 antibody [EPR19759]
See all PD-L1 primary antibodies -
Description
HRP Rabbit monoclonal [EPR19759] to PD-L1 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: CHO-PDL1
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19759 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-PD-L1 antibody [EPR19759] (ab213524)
- Alexa Fluor® 488 Anti-PD-L1 antibody [EPR19759] (ab214958)
- Alexa Fluor® 647 Anti-PD-L1 antibody [EPR19759] (ab215251)
- Alexa Fluor® 594 Anti-PD-L1 antibody [EPR19759] (ab216742)
- Alexa Fluor® 568 Anti-PD-L1 antibody [EPR19759] (ab220314)
- Anti-PD-L1 antibody [EPR19759] - BSA and Azide free (ab221612)
- Anti-PD-L1 antibody [EPR19759] - Low endotoxin, Azide free (ab246695)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000.
Treat samples with PNGase F to confirm the specificity of bands if necessary. PMID: 31327656 The observed band size is not exactly as predicted MWs in WB due to the different forms and modifications of PD-L1. Hu Isoform I-3: 20-33 kDa (predicted) Actual band size in WB is between 40-60 kDa. Positive Control: B-CPAP, MD-MB-231, U87-MG Negative Control: SW480 |
| Notes |
|---|
|
WB
1/5000. Treat samples with PNGase F to confirm the specificity of bands if necessary. PMID: 31327656 The observed band size is not exactly as predicted MWs in WB due to the different forms and modifications of PD-L1. Hu Isoform I-3: 20-33 kDa (predicted) Actual band size in WB is between 40-60 kDa. Positive Control: B-CPAP, MD-MB-231, U87-MG Negative Control: SW480 |
Target
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Function
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. -
Tissue specificity
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane and Endomembrane system. - Information by UniProt
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Database links
- Entrez Gene: 29126 Human
- Omim: 605402 Human
- SwissProt: Q9NZQ7 Human
- Unigene: 521989 Human
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Alternative names
- B7 H antibody
- B7 H1 antibody
- B7 homolog 1 antibody
see all
Images
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Western blot - HRP Anti-PD-L1 antibody [EPR19759] (ab215349)All lanes : HRP Anti-PD-L1 antibody [EPR19759] (ab215349) at 1/5000 dilution
Lane 1 : CHO-S
Lane 2 : CHO-PDL1
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 40-52 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab215349 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-PD-L1 antibody [EPR19759] (ab215349)](https://www.abcam.com/ps/products/215/ab215349/Images/ab215349-2-benefits-of-recombinant-antibodies.png)
HRP Anti-PD-L1 antibody [EPR19759] (ab215349)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab215349 has not yet been referenced specifically in any publications.