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    hrp-pd-l1-antibody-epr19759-ab215349.pdf

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Immunology Cell Type Markers CD Non-lineage
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RecombinantRabMAb

Recombinant HRP Anti-PD-L1 antibody [EPR19759] (ab215349)

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  • SDS
  • Certificate of Compliance
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Western blot - HRP Anti-PD-L1 antibody [EPR19759] (ab215349)
  • HRP Anti-PD-L1 antibody [EPR19759] (ab215349)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR19759] to PD-L1
  • Suitable for: WB
  • Reacts with: Human
  • Conjugation: HRP

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free Unconjugated

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HRP Conjugation Kit - Lightning-Link® (ab102890)
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Anti-PD-L1 antibody [EPR19759] - BSA and Azide free (ab221612)
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Anti-PD-L1 antibody [EPR19759] - Low endotoxin, Azide free (ab246695)

View more associated products

Overview

  • Product name

    HRP Anti-PD-L1 antibody [EPR19759]
    See all PD-L1 primary antibodies
  • Description

    HRP Rabbit monoclonal [EPR19759] to PD-L1
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: CHO-PDL1
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin 300 Solution
    Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19759
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Non-lineage
    • Immunology
    • Immune System Diseases
    • Autoimmune
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens
    • Cancer
    • Tumor biomarkers
    • Tumor antigens

Associated products

  • Alternative Versions

    • Anti-PD-L1 antibody [EPR19759] (ab213524)
    • Alexa Fluor® 488 Anti-PD-L1 antibody [EPR19759] (ab214958)
    • Alexa Fluor® 647 Anti-PD-L1 antibody [EPR19759] (ab215251)
    • Alexa Fluor® 594 Anti-PD-L1 antibody [EPR19759] (ab216742)
    • Alexa Fluor® 568 Anti-PD-L1 antibody [EPR19759] (ab220314)
    • Anti-PD-L1 antibody [EPR19759] - BSA and Azide free (ab221612)
    • Anti-PD-L1 antibody [EPR19759] - Low endotoxin, Azide free (ab246695)
  • Isotype control

    • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)
  • Recombinant Protein

    • Recombinant human PD-L1 protein (Active) (ab167713)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab215349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000.

Treat samples with PNGase F to confirm the specificity of bands if necessary. PMID: 31327656 The observed band size is not exactly as predicted MWs in WB due to the different forms and modifications of PD-L1. Hu Isoform I-3: 20-33 kDa (predicted) Actual band size in WB is between 40-60 kDa. Positive Control: B-CPAP, MD-MB-231, U87-MG Negative Control: SW480

Notes
WB
1/5000.

Treat samples with PNGase F to confirm the specificity of bands if necessary. PMID: 31327656 The observed band size is not exactly as predicted MWs in WB due to the different forms and modifications of PD-L1. Hu Isoform I-3: 20-33 kDa (predicted) Actual band size in WB is between 40-60 kDa. Positive Control: B-CPAP, MD-MB-231, U87-MG Negative Control: SW480

Target

  • Function

    Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
  • Tissue specificity

    Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane and Endomembrane system.
  • Target information above from: UniProt accession Q9NZQ7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 29126 Human
    • Omim: 605402 Human
    • SwissProt: Q9NZQ7 Human
    • Unigene: 521989 Human
    • Alternative names

      • B7 H antibody
      • B7 H1 antibody
      • B7 homolog 1 antibody
      • B7-H1 antibody
      • B7H antibody
      • B7H1 antibody
      • CD 274 antibody
      • CD-274 antibody
      • CD274 antibody
      • CD274 antigen antibody
      • CD274 molecule antibody
      • MGC142294 antibody
      • MGC142296 antibody
      • OTTHUMP00000021029 antibody
      • PD L1 antibody
      • PD-L1 antibody
      • PD1L1_HUMAN antibody
      • PDCD1 ligand 1 antibody
      • PDCD1L1 antibody
      • PDCD1LG1 antibody
      • PDL 1 antibody
      • PDL1 antibody
      • Programmed cell death 1 ligand 1 antibody
      • Programmed death ligand 1 antibody
      • RGD1566211 antibody
      see all

    Images

    • Western blot - HRP Anti-PD-L1 antibody [EPR19759] (ab215349)
      Western blot - HRP Anti-PD-L1 antibody [EPR19759] (ab215349)
      All lanes : HRP Anti-PD-L1 antibody [EPR19759] (ab215349) at 1/5000 dilution

      Lane 1 : CHO-S
      Lane 2 : CHO-PDL1

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Performed under reducing conditions.

      Observed band size: 40-52 kDa why is the actual band size different from the predicted?


      Exposure time: 8 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab215349 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • HRP Anti-PD-L1 antibody [EPR19759] (ab215349)
      HRP Anti-PD-L1 antibody [EPR19759] (ab215349)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab215349? Please let us know so that we can cite the reference in this datasheet.

    ab215349 has not yet been referenced specifically in any publications.

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