Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR19759] to PD-L1
- Suitable for: WB
- Conjugation: HRP
Product nameHRP Anti-PD-L1 antibody [EPR19759]
See all PD-L1 primary antibodies
DescriptionHRP Rabbit monoclonal [EPR19759] to PD-L1
Tested applicationsSuitable for: WBmore details
Predicted to work with: Human
Synthetic peptide. within Human PD-L1 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q9NZQ7
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
- Anti-PD-L1 antibody [EPR19759] (ab213524)
- Alexa Fluor® 488 Anti-PD-L1 antibody [EPR19759] (ab214958)
- Alexa Fluor® 647 Anti-PD-L1 antibody [EPR19759] (ab215251)
- Alexa Fluor® 594 Anti-PD-L1 antibody [EPR19759] (ab216742)
- Alexa Fluor® 568 Anti-PD-L1 antibody [EPR19759] (ab220314)
- Anti-PD-L1 antibody [EPR19759] - BSA and Azide free (ab221612)
- Anti-PD-L1 antibody [EPR19759] - Low endotoxin, Azide free (ab246695)
Our Abpromise guarantee covers the use of ab215349 in the following tested applications.
|WB||1/5000. Detects a band of approximately 40-52 kDa (predicted molecular weight: 33 kDa).
Treat samples with PNGase F to confirm the specificity of bands if necessary. PMID: 31327656
The observed band size is not exactly as predicted MWs in WB due to the different forms and modifications of PD-L1.
Hu Isoform I-3: 20-33 kDa (predicted)
Actual band size in WB is between 40-60 kDa.
Positive Control: B-CPAP, MD-MB-231, U87-MG
Negative Control: SW480
FunctionInvolved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
Tissue specificityHighly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
Sequence similaritiesBelongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell membrane and Endomembrane system.
- Information by UniProt
- B7 H antibody
- B7 H1 antibody
- B7 homolog 1 antibody
All lanes : HRP Anti-PD-L1 antibody [EPR19759] (ab215349) at 1/5000 dilution
Lane 1 : CHO-S
Lane 2 : CHO-PDL1
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 40-52 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab215349 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab215349 has not yet been referenced specifically in any publications.