Key features and details
- HRP Rabbit polyclonal to Phosphoserine
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
Product nameHRP Anti-Phosphoserine antibody
See all Phosphoserine primary antibodies
DescriptionHRP Rabbit polyclonal to Phosphoserine
SpecificitySpecifically recognizes both free-phosphoserine, serine-phosphorylated peptide and proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Has slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Species independent
BSA and KLH-phosphoserine conjugates.
- Use Mouse brain extract for Western Blotting or synthetic phosphopeptide or phosvitin for ELISA.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 6.00
Constituent: Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesImmunoaffinity chromatography with phosphoserine-agarose.
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab9334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding.|
|ELISA||Use a concentration of 1 µg/ml.|
RelevanceChanges in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
- phospho-Ser antibody
- pS antibody
- pSER antibody
ab9334 has been referenced in 9 publications.
- Martínez-Florensa M et al. Serine residues in the LAT adaptor are essential for TCR-dependent signal transduction. J Leukoc Biol 89:63-73 (2011). WB . PubMed: 20940326
- Zang ZJ et al. Identification of PP2A as a novel interactor and regulator of TRIP-Br1. Cell Signal 21:34-42 (2009). WB . PubMed: 18940248
- Selvarajoo K et al. Signaling flux redistribution at toll-like receptor pathway junctions. PLoS One 3:e3430 (2008). WB . PubMed: 18927610
- Bokui N et al. Involvement of MAPK signaling molecules and Runx2 in the NELL1-induced osteoblastic differentiation. FEBS Lett 582:365-71 (2008). PubMed: 18082140
- McLaughlin NJ et al. Platelet-activating factor-mediated endosome formation causes membrane translocation of p67phox and p40phox that requires recruitment and activation of p38 MAPK, Rab5a, and phosphatidylinositol 3-kinase in human neutrophils. J Immunol 180:8192-203 (2008). WB . PubMed: 18523285
- Mitchell JE et al. The presumptive phosphatidylserine receptor is dispensable for innate anti-inflammatory recognition and clearance of apoptotic cells. J Biol Chem 281:5718-25 (2006). WB . PubMed: 16317002
- Virok DP et al. Chlamydial infection induces pathobiotype-specific protein tyrosine phosphorylation in epithelial cells. Infect Immun 73:1939-46 (2005). WB . PubMed: 15784533
- Scott GK et al. Vitamin K3 (menadione)-induced oncosis associated with keratin 8 phosphorylation and histone H3 arylation. Mol Pharmacol 68:606-15 (2005). PubMed: 15939799
- Luettich K & Schmidt C TGFbeta1 activates c-Jun and Erk1 via alphaVbeta6 integrin. Mol Cancer 2:33 (2003). PubMed: 14572313