HRP Anti-Phosphotyrosine antibody (ab9329)
Key features and details
- HRP Rabbit polyclonal to Phosphotyrosine
- Suitable for: WB, IP, ELISA
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-Phosphotyrosine antibody
See all Phosphotyrosine primary antibodies -
Description
HRP Rabbit polyclonal to Phosphotyrosine -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WB, IP, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Phosphotyrosine conjugated to keyhole limpet haemocyanin.
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Positive control
- Use Vanadium treated cell culture (30 min) for Western Blotting and Tyrosine-phosphorylated peptide for ELISA.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Immunoaffinity chromatography with phosphotyrosine-agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Related Products
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- Immunoassay Blocking Buffer (ab171534)
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration.
|
| IP |
Use at an assay dependent concentration.
|
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| ELISA |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
Target
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Relevance
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
Images
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Western blot - HRP Anti-Phosphotyrosine antibody (ab9329)
Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)
Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab9329 has been referenced in 1 publication.
- McLaughlin NJ et al. Platelet-Activating Factor-Mediated Endosome Formation Causes Membrane Translocation of p67phox and p40phox That Requires Recruitment and Activation of p38 MAPK, Rab5a, and Phosphatidylinositol 3-Kinase in Human Neutrophils. J Immunol 180:8192-203 (2008). WB . PubMed: 18523285
