HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)
![Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)](https://www.abcam.com/ps/products/16/ab16389/Images/ab16389-24967-anti-phosphotyrosine-antibody-py20-hrp-western-blot.jpg "Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)")
Key features and details
- HRP Mouse monoclonal [PY20] to Phosphotyrosine
- Suitable for: IHC-P, WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG2b
Overview
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Product name
HRP Anti-Phosphotyrosine antibody [PY20]
See all Phosphotyrosine primary antibodies -
Description
HRP Mouse monoclonal [PY20] to Phosphotyrosine -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Full length native protein (purified) corresponding to Phosphotyrosine.
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Positive control
- This antibody gave a positive signal in NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate.
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General notes
This antibody is known to be inhibited by divalent cations (>1mM) and high salt concentrations (>0.2M).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
PY20 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Related Products
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Applications
Our Abpromise guarantee covers the use of ab16389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/5000. |
Target
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Relevance
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
Images
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Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)HRP Anti-Phosphotyrosine antibody [PY20] (ab16389) at 1/5000 dilution + NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds -
![Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)](https://www.abcam.com/ps/products/16/ab16389/Images/ab16389-24966-anti-phosphotyrosine-antibody-py20-hrp-western-blot.jpg)
Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)HRP Anti-Phosphotyrosine antibody [PY20] (ab16389) at 1/5000 dilution ((BLOCKED WITH 3% MILK)) + NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
Protocols
References (11)
ab16389 has been referenced in 11 publications.
- Hirakata S et al. Genetic Deletion of Socs3 in Smooth Muscle Cells Ameliorates Aortic Dissection in Mice. JACC Basic Transl Sci 5:126-144 (2020). PubMed: 32140621
- Juettner VV et al. VE-PTP stabilizes VE-cadherin junctions and the endothelial barrier via a phosphatase-independent mechanism. J Cell Biol 218:1725-1742 (2019). PubMed: 30948425
- Rahman MS et al. Effect of antioxidants on BPA-induced stress on sperm function in a mouse model. Sci Rep 9:10584 (2019). PubMed: 31332285
- Nagala M et al. Expression of Siglec-E Alters the Proteome of Lipopolysaccharide (LPS)-Activated Macrophages but Does Not Affect LPS-Driven Cytokine Production or Toll-Like Receptor 4 Endocytosis. Front Immunol 8:1926 (2017). PubMed: 29379501
- Fujikawa A et al. Targeting PTPRZ inhibits stem cell-like properties and tumorigenicity in glioblastoma cells. Sci Rep 7:5609 (2017). PubMed: 28717188
