HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)
![Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)](https://www.abcam.com/ps/products/16/ab16389/Images/ab16389-24967-anti-phosphotyrosine-antibody-py20-hrp-western-blot.jpg "Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)")
Key features and details
- HRP Mouse monoclonal [PY20] to Phosphotyrosine
- Suitable for: IHC-P, WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
HRP Anti-Phosphotyrosine antibody [PY20]
See all Phosphotyrosine primary antibodies -
Description
HRP Mouse monoclonal [PY20] to Phosphotyrosine -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Full length native protein (purified) corresponding to Phosphotyrosine.
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Positive control
- This antibody gave a positive signal in NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate.
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General notes
This antibody is known to be inhibited by divalent cations (>1mM) and high salt concentrations (>0.2M).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
PY20 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Related Products
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
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- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/5000.
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| Notes |
|---|
|
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/5000. |
Target
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Relevance
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
Images
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Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)HRP Anti-Phosphotyrosine antibody [PY20] (ab16389) at 1/5000 dilution + NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds -
![Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)](https://www.abcam.com/ps/products/16/ab16389/Images/ab16389-24966-anti-phosphotyrosine-antibody-py20-hrp-western-blot.jpg)
Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)HRP Anti-Phosphotyrosine antibody [PY20] (ab16389) at 1/5000 dilution ((BLOCKED WITH 3% MILK)) + NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab16389 has been referenced in 15 publications.
- Hirakata S et al. Genetic Deletion of Socs3 in Smooth Muscle Cells Ameliorates Aortic Dissection in Mice. JACC Basic Transl Sci 5:126-144 (2020). PubMed: 32140621
- Bae JW et al. Vanadium adversely affects sperm motility and capacitation status via protein kinase A activity and tyrosine phosphorylation. Reprod Toxicol 96:195-201 (2020). PubMed: 32659260
- Hayes BH et al. Macrophages show higher levels of engulfment after disruption of cis interactions between CD47 and the checkpoint receptor SIRPa. J Cell Sci 133:N/A (2020). PubMed: 31964705
- Tanga N et al. The PTN-PTPRZ signal activates the AFAP1L2-dependent PI3K-AKT pathway for oligodendrocyte differentiation: Targeted inactivation of PTPRZ activity in mice. Glia 67:967-984 (2019). PubMed: 30667096
- Fujikawa A et al. A head-to-toe dimerization has physiological relevance for ligand-induced inactivation of protein tyrosine receptor type Z. J Biol Chem 294:14953-14965 (2019). PubMed: 31416834