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Epigenetics and Nuclear Signaling Histones H3 Methylated
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RecombinantRabMAb

Recombinant HRP Anti-PKC alpha + beta + gamma antibody [EPR16898] (ab205791)

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  • SDS
  • Certificate of Compliance
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Western blot - HRP Anti-PKC alpha + beta + gamma antibody [EPR16898] (ab205791)
  • HRP Anti-PKC alpha + beta + gamma antibody [EPR16898] (ab205791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR16898] to PKC alpha + beta + gamma
  • Suitable for: WB
  • Reacts with: Rat, Human
  • Conjugation: HRP

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Conjugation
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Overview

  • Product name

    HRP Anti-PKC alpha + beta + gamma antibody [EPR16898]
    See all PKC alpha + beta + gamma primary antibodies
  • Description

    HRP Rabbit monoclonal [EPR16898] to PKC alpha + beta + gamma
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment within Human PKC alpha + beta + gamma aa 300-500. The exact sequence is proprietary. Also SwissProt IDs: P17252 = Protein kinase C alpha type. P05129 = Protein kinase C gamma type.
    Database link: P05771

  • Positive control

    • WB: Human fetal brain and rat brain tissue lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: 1% BSA, 30% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16898
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Methylated
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKC
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • Other

Associated products

  • Alternative Versions

    • Anti-PKC antibody [EPR16898] (ab179522)
    • Alexa Fluor® 647 Anti-PKC alpha + beta + gamma antibody [EPR16898] (ab205478)
    • PE Anti-PKC alpha + beta + gamma antibody [EPR16898] (ab211709)
    • Anti-PKC antibody [EPR16898] - BSA and Azide free (ab250108)
  • Isotype control

    • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)

Applications

Our Abpromise guarantee covers the use of ab205791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 80 kDa (predicted molecular weight: 77 kDa).

Target

  • Function

    Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells. In intestinal cells stimulated by the phorbol ester PMA, can trigger a cell cycle arrest program which is associated with the accumulation of the hyper-phosphorylated growth-suppressive form of RB1 and induction of the CDK inhibitors CDKN1A and CDKN1B. Exhibits anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3, and in leukemia cells mediates anti-apoptotic action by phosphorylating BCL2. During macrophage differentiation induced by macrophage colony-stimulating factor (CSF1), is translocated to the nucleus and is associated with macrophage development. After wounding, translocates from focal contacts to lamellipodia and participates in the modulation of desmosomal adhesion. Plays a role in cell motility by phosphorylating CSPG4, which induces association of CSPG4 with extensive lamellipodia at the cell periphery and polarization of the cell accompanied by increases in cell motility. Is highly expressed in a number of cancer cells where it can act as a tumor promoter and is implicated in malignant phenotypes of several tumors such as gliomas and breast cancers. Negatively regulates myocardial contractility and positively regulates angiogenesis, platelet aggregation and thrombus formation in arteries. Mediates hypertrophic growth of neonatal cardiomyocytes, in part through a MAPK1/3 (ERK1/2)-dependent signaling pathway, and upon PMA treatment, is required to induce cardiomyocyte hypertrophy up to heart failure and death, by increasing protein synthesis, protein-DNA ratio and cell surface area. Regulates cardiomyocyte function by phosphorylating cardiac troponin T (TNNT2/CTNT), which induces significant reduction in actomyosin ATPase activity, myofilament calcium sensitivity and myocardial contractility. In angiogenesis, is required for full endothelial cell migration, adhesion to vitronectin (VTN), and vascular endothelial growth factor A (VEGFA)-dependent regulation of kinase activation and vascular tube formation. Involved in the stabilization of VEGFA mRNA at post-transcriptional level and mediates VEGFA-induced cell proliferation. In the regulation of calcium-induced platelet aggregation, mediates signals from the CD36/GP4 receptor for granule release, and activates the integrin heterodimer ITGA2B-ITGB3 through the RAP1GAP pathway for adhesion. During response to lipopolysaccharides (LPS), may regulate selective LPS-induced macrophage functions involved in host defense and inflammation. But in some inflammatory responses, may negatively regulate NF-kappa-B-induced genes, through IL1A-dependent induction of NF-kappa-B inhibitor alpha (NFKBIA/IKBA). Upon stimulation with 12-O-tetradecanoylphorbol-13-acetate (TPA), phosphorylates EIF4G1, which modulates EIF4G1 binding to MKNK1 and may be involved in the regulation of EIF4E phosphorylation. Phosphorylates KIT, leading to inhibition of KIT activity. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Cellular localization

    Cytoplasm. Cell membrane. Mitochondrion membrane. Nucleus.
  • Target information above from: UniProt accession P17252 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5578 Human
    • Entrez Gene: 5579 Human
    • Entrez Gene: 5582 Human
    • Entrez Gene: 18750 Mouse
    • Entrez Gene: 18751 Mouse
    • Entrez Gene: 18752 Mouse
    • Entrez Gene: 24681 Rat
    • Entrez Gene: 25023 Rat
    • Omim: 176960 Human
    • Omim: 176980 Human
    • SwissProt: P05129 Human
    • SwissProt: P05771 Human
    • SwissProt: P17252 Human
    • SwissProt: P20444 Mouse
    • SwissProt: P68404 Mouse
    • SwissProt: P05696 Rat
    • SwissProt: P63319 Rat
    • SwissProt: P68403 Rat
    • Unigene: 531704 Human
    • Unigene: 708867 Human
    • Unigene: 222178 Mouse
    • Unigene: 86669 Rat
    see all
  • Form

    Specific for both beta 1 and 2 isoforms
  • Alternative names

    • AAG6 antibody
    • Aging associated gene 6 antibody
    • KPCA_HUMAN antibody
    • PKC beta antibody
    • PKC gamma antibody
    • PKC-A antibody
    • PKC-alpha antibody
    • PKCA antibody
    • PKCB antibody
    • PKCC antibody
    • PKCG antibody
    • PRKACA antibody
    • PRKCA antibody
    • PRKCB antibody
    • PRKCB1 antibody
    • PRKCB2 antibody
    • PRKCG antibody
    • Protein kinase C alpha antibody
    • Protein kinase C alpha type antibody
    • Protein kinase C beta antibody
    • Protein kinase C beta type antibody
    • Protein kinase C gamma antibody
    • Protein kinase C gamma type antibody
    • SCA14 antibody
    see all

Images

  • Western blot - HRP Anti-PKC alpha + beta + gamma antibody [EPR16898] (ab205791)
    Western blot - HRP Anti-PKC alpha + beta + gamma antibody [EPR16898] (ab205791)
    All lanes : HRP Anti-PKC alpha + beta + gamma antibody [EPR16898] (ab205791) at 1/5000 dilution

    Lane 1 : Brain (Human) Tissue Lysate - fetal normal tissue at 20 µg
    Lane 2 : Brain (Rat) Tissue Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 77 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?


    Exposure time: 4 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205791 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • HRP Anti-PKC alpha + beta + gamma antibody [EPR16898] (ab205791)
    HRP Anti-PKC alpha + beta + gamma antibody [EPR16898] (ab205791)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab205791? Please let us know so that we can cite the reference in this datasheet.

    ab205791 has not yet been referenced specifically in any publications.

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