Key features and details
- HRP Rabbit monoclonal [EPR6141] to Placental alkaline phosphatase (PLAP)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Conjugation: HRP
Product nameHRP Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141]
See all Placental alkaline phosphatase (PLAP) primary antibodies
DescriptionHRP Rabbit monoclonal [EPR6141] to Placental alkaline phosphatase (PLAP)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Placental alkaline phosphatase (PLAP) aa 150-250 (extracellular). The exact sequence is proprietary.
- WB: Human placenta tissue lysate. IHC-P: Human placenta tissue (normal).
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Dissociation constant (KD)KD = 5.10 x 10 -11 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab198554 in the following tested applications.
|WB||1/5000. Detects a band of approximately 70 kDa (predicted molecular weight: 58 kDa).|
Sequence similaritiesBelongs to the alkaline phosphatase family.
Cellular localizationCell membrane.
- Information by UniProt
- Alkaline phosphatase antibody
- Alkaline phosphatase placental antibody
- Alkaline phosphatase placental type antibody
HRP Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab198554) at 1/5000 dilution + Human placenta tissue lysate - total protein (ab29745) at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab198554 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
IHC image of Placental alkaline phosphatase (PLAP) staining in a section of formalin-fixed paraffin-embedded normal human placenta*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab198554 at 1/1000 dilution for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
ab198554 has not yet been referenced specifically in any publications.