Recombinant HRP Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab198554)
![Western blot - HRP Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab198554)](https://www.abcam.com/ps/products/198/ab198554/Images/ab198554-244661-WBab1985541.jpg "Western blot - HRP Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab198554)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR6141] to Placental alkaline phosphatase (PLAP)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Conjugation: HRP
Overview
-
Product name
HRP Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141]
See all Placental alkaline phosphatase (PLAP) primary antibodies -
Description
HRP Rabbit monoclonal [EPR6141] to Placental alkaline phosphatase (PLAP) -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Placental alkaline phosphatase (PLAP) aa 150-250 (extracellular). The exact sequence is proprietary.
-
Positive control
- WB: Human placenta tissue lysate. IHC-P: Human placenta tissue (normal).
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Dissociation constant (KD)
KD = 5.10 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6141 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab198554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/1000. | |
| WB | 1/5000. Detects a band of approximately 70 kDa (predicted molecular weight: 58 kDa). |
Target
-
Sequence similarities
Belongs to the alkaline phosphatase family. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 250 Human
- Omim: 171800 Human
- SwissProt: P05187 Human
- Unigene: 284255 Human
-
Alternative names
- Alkaline phosphatase antibody
- Alkaline phosphatase placental antibody
- Alkaline phosphatase placental type antibody
see all
Images
-
Western blot - HRP Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab198554)HRP Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab198554) at 1/5000 dilution + Human placenta tissue lysate - total protein (ab29745) at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab198554 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab198554)](https://www.abcam.com/ps/products/198/ab198554/Images/ab198554-244536-AP22578941.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab198554)IHC image of Placental alkaline phosphatase (PLAP) staining in a section of formalin-fixed paraffin-embedded normal human placenta*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab198554 at 1/1000 dilution for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
-
![HRP Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab198554)](https://www.abcam.com/ps/products/198/ab198554/Images/ab198554-3-benefits-of-recombinant-antibodies.png)
HRP Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab198554)
References (0)
ab198554 has not yet been referenced specifically in any publications.
