Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [PEG-B-47] to Polyethylene glycol
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Conjugation: HRP
Product nameHRP Anti-Polyethylene glycol antibody [PEG-B-47]
See all Polyethylene glycol primary antibodies
DescriptionHRP Rabbit monoclonal [PEG-B-47] to Polyethylene glycol
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule corresponding to Polyethylene glycol conjugated to Keyhole Limpet Haemocyanin (KLH). KLH-Polyethylene glycol with a terminal methoxy group.
- WB: BSA-PEG
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Dissociation constant (KD)KD = 2.41 x 10 -9 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab202640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
Do Not Use TBS As Buffer
- PEG antibody
HRP Anti-Polyethylene glycol antibody [PEG-B-47] (ab202640) at 1/200 dilution + BSA-PEG at 15 µg
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 3 minutes
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Sandwich ELISA antigen dose-response curve using ab202640.
Primary antibody concentration - ab202640 (500ng/ml and 250ng/ml).
Antigen - MIgG-PEG 5K (0-1000ng/ml).
Coated antibody - Goat anti-Mouse IgG (1000ng/ml).
Direct ELISA antibody dose-response curve using ab202640. Primary antibody concentration range 0-1000ng/ml, antigen (MIgG-PEG 5K) concentration range 0-500ng/ml.
ab202640 has been referenced in 1 publication.
- Triplett TA et al. Reversal of indoleamine 2,3-dioxygenase-mediated cancer immune suppression by systemic kynurenine depletion with a therapeutic enzyme. Nat Biotechnol 36:758-764 (2018). PubMed: 30010674