Recombinant HRP Anti-Polyethylene glycol antibody [PEG-B-47] (ab202640)
![Western blot - HRP Anti-Polyethylene glycol antibody [PEG-B-47] (ab202640)](https://www.abcam.com/ps/products/202/ab202640/Images/ab202640-258825-anti-polyethylene-glycol-antibody-peg-b-47-hrp-western-blot.jpg "Western blot - HRP Anti-Polyethylene glycol antibody [PEG-B-47] (ab202640)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [PEG-B-47] to Polyethylene glycol
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Conjugation: HRP
Related conjugates and formulations
Overview
-
Product name
HRP Anti-Polyethylene glycol antibody [PEG-B-47]
See all Polyethylene glycol primary antibodies -
Description
HRP Rabbit monoclonal [PEG-B-47] to Polyethylene glycol -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Polyethylene glycol conjugated to keyhole limpet haemocyanin. KLH-Polyethylene glycol with a terminal methoxy group.
-
Positive control
- WB: BSA-PEG
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Dissociation constant (KD)
KD = 2.41 x 10 -9 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
PEG-B-47 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab202640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/200.
|
|
| ELISA |
Use at an assay dependent concentration.
Do Not Use TBS As Buffer |
| Notes |
|---|
|
WB
1/200. |
|
ELISA
Use at an assay dependent concentration. Do Not Use TBS As Buffer |
Target
-
Alternative names
- PEG antibody
Images
-
Western blot - HRP Anti-Polyethylene glycol antibody [PEG-B-47] (ab202640)HRP Anti-Polyethylene glycol antibody [PEG-B-47] (ab202640) at 1/200 dilution + BSA-PEG at 15 µg
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 3 minutesBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
![ELISA - HRP Anti-Polyethylene glycol antibody [PEG-B-47] (ab202640)](https://www.abcam.com/ps/products/202/ab202640/Images/ab202640-269632-anti-polyethylene-glycol-antibody-peg-b-47-hrp-elisa.jpg)
ELISA - HRP Anti-Polyethylene glycol antibody [PEG-B-47] (ab202640)Sandwich ELISA antigen dose-response curve using ab202640.
Primary antibody concentration - ab202640 (500ng/ml and 250ng/ml).
Antigen - MIgG-PEG 5K (0-1000ng/ml).
Coated antibody - Goat anti-Mouse IgG (1000ng/ml). -
![ELISA - HRP Anti-Polyethylene glycol antibody [PEG-B-47] (ab202640)](https://www.abcam.com/ps/products/202/ab202640/Images/ab202640-269631-anti-polyethylene-glycol-antibody-peg-b-47-hrp-elisa.jpg)
ELISA - HRP Anti-Polyethylene glycol antibody [PEG-B-47] (ab202640)Direct ELISA antibody dose-response curve using ab202640. Primary antibody concentration range 0-1000ng/ml, antigen (MIgG-PEG 5K) concentration range 0-500ng/ml.
-
![HRP Anti-Polyethylene glycol antibody [PEG-B-47] (ab202640)](https://www.abcam.com/ps/products/202/ab202640/Images/ab202640-5-benefits-of-recombinant-antibodies.png)
HRP Anti-Polyethylene glycol antibody [PEG-B-47] (ab202640)
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (1)
ab202640 has been referenced in 1 publication.
- Triplett TA et al. Reversal of indoleamine 2,3-dioxygenase-mediated cancer immune suppression by systemic kynurenine depletion with a therapeutic enzyme. Nat Biotechnol 36:758-764 (2018). PubMed: 30010674