HRP Anti-polyHistidine antibody [HIS-1] (ab49781)
Key features and details
- HRP Mouse monoclonal [HIS-1] to polyHistidine
- Suitable for: ELISA, Dot blot, WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
HRP Anti-polyHistidine antibody [HIS-1] -
Description
HRP Mouse monoclonal [HIS-1] to polyHistidine -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: ELISA, Dot blot, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant polyHistidine tagged fusion protein.
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Positive control
- Bacteria lysates expressing a recombinant Histidine tagged fusion protein.
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General notes
Monoclonal antibody reacting specifically with polyHistidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidine tagged fusion protein expressing vectors.
The conjugate was prepared by conjugation of HRP to Mouse monoclonal anti-polyHistidine purified from ascites fluid of the HIS-1 hybridoma. Molar ratio Ab/Enzyme: 0.8 to 1.5 Enzyme activity: at least 400 U/ml
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.0268% PBS, 1% BSA -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
Purified Immunoglobulin -
Primary antibody notes
Monoclonal antibody reacting specifically with polyHistidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidine tagged fusion protein expressing vectors. -
Clonality
Monoclonal -
Clone number
HIS-1 -
Isotype
IgG2a -
Research areas
Associated products
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Related Products
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab49781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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Dot blot |
1/2000.
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WB |
1/2000.
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Notes |
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ELISA
Use at an assay dependent concentration. |
Dot blot
1/2000. |
WB
1/2000. |
Target
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Relevance
Recombinant DNA technology enables the attachment of genes of interest to specific sequences or genes that can provide 'affinity handles' (tags) designed to enable the selective identification of the protein of interest. These sequences of tails or tags are genetically engineered away from the protein active site, by insertion at the N or C terminus. It has been reported that the addition of a consecutive Histidine amino acid residue tail creates a stable fusion product that does not appear to interfere with the bioactivity of the protein or with the biodistribution of the Histidine tagged product.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab49781 has been referenced in 2 publications.
- Shin J et al. Characterization of a single chain variable fragment of nivolumab that targets PD-1 and blocks PD-L1 binding. Protein Expr Purif 177:105766 (2021). PubMed: 32987122
- Kim YJ et al. Molecular and Structural Characterization of the Tegumental 20.6-kDa Protein in Clonorchis sinensis as a Potential Druggable Target. Int J Mol Sci 18:N/A (2017). PubMed: 28273846