Recombinant HRP Anti-PP2A alpha + beta antibody [E155] (ab196979)
![Western blot - HRP Anti-PP2A alpha + beta antibody [E155] (ab196979)](https://www.abcam.com/ps/products/196/ab196979/Images/ab196979-238294-anti-pp2a-alpha-beta-antibody-e155-hrp-western-blot.jpg "Western blot - HRP Anti-PP2A alpha + beta antibody [E155] (ab196979)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [E155] to PP2A alpha + beta
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-PP2A alpha + beta antibody [E155]
See all PP2A alpha + beta primary antibodies -
Description
HRP Rabbit monoclonal [E155] to PP2A alpha + beta -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PP2A alpha + beta aa 250 to the C-terminus.
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Positive control
- WB: A431 whole cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E155 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Biotin Anti-PP2A alpha + beta antibody [E155] (ab197706)
- Anti-PP2A alpha + beta antibody [E155] - BSA and Azide free (ab226007)
- Alexa Fluor® 488 Anti-PP2A alpha + beta antibody [E155] (ab252049)
- Alexa Fluor® 647 Anti-PP2A alpha + beta antibody [E155] (ab252050)
- Anti-PP2A alpha + beta antibody [E155] (ab32104)
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab196979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). |
Target
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Function
PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'. -
Sequence similarities
Belongs to the PPP phosphatase family. PP-1 subfamily. -
Post-translational
modificationsReversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly, enhancing the affinity of the PP2A core enzyme for some, but not all, regulatory subunits. It varies during the cell cycle.
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. -
Cellular localization
Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2. - Information by UniProt
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Database links
- Entrez Gene: 5515 Human
- Entrez Gene: 5516 Human
- Entrez Gene: 19052 Mouse
- Entrez Gene: 19053 Mouse
- Entrez Gene: 24672 Rat
- Entrez Gene: 24673 Rat
- Omim: 176915 Human
- Omim: 176916 Human
see all -
Alternative names
- PP2A A antibody
- PP2A alpha antibody
- PP2A B antibody
see all
Images
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Western blot - HRP Anti-PP2A alpha + beta antibody [E155] (ab196979)HRP Anti-PP2A alpha + beta antibody [E155] (ab196979) at 1/5000 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196979 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-PP2A alpha + beta antibody [E155] (ab196979)](https://www.abcam.com/ps/products/196/ab196979/Images/ab196979-2-benefits-of-recombinant-antibodies.png)
HRP Anti-PP2A alpha + beta antibody [E155] (ab196979)
References (0)
ab196979 has not yet been referenced specifically in any publications.
