Recombinant HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)
![Western blot - HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)](https://www.abcam.com/ps/products/211/ab211372/Images/ab211372-282234-anti-ppp1ca-1cb-antibody-ep1511y-hrp-western-blot.jpg "Western blot - HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP1511Y] to PPP1CA + PPP1CB
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y]
See all PPP1CA + PPP1CB primary antibodies -
Description
HRP Rabbit monoclonal [EP1511Y] to PPP1CA + PPP1CB -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa whole cell and Mouse Brain and Rat Brain tissue lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1511Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).
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| Notes |
|---|
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WB
1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa). |
Target
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Cellular localization
Cytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus. -
Database links
- Entrez Gene: 5499 Human
- Entrez Gene: 5500 Human
- Entrez Gene: 19045 Mouse
- Entrez Gene: 19046 Mouse
- Entrez Gene: 24668 Rat
- Entrez Gene: 25594 Rat
- Omim: 176875 Human
- Omim: 600590 Human
see all -
Alternative names
- PP-1A antibody
- PP-1B antibody
- PP1A antibody
see all
Images
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Western blot - HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)All lanes : HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372) at 1/5000 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 :HeLa whole cell lysate (ab150035)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab211372 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)](https://www.abcam.com/ps/products/211/ab211372/Images/ab211372-2-benefits-of-recombinant-antibodies.png)
HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab211372 has been referenced in 1 publication.
- Hu Z et al. Protein phosphatase 1 catalyzes HBV core protein dephosphorylation and is co-packaged with viral pregenomic RNA into nucleocapsids. PLoS Pathog 16:e1008669 (2020). PubMed: 32702076