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  1. Link

    hrp-ppp1ca--ppp1cb-antibody-ep1511y-ab211372.pdf

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Cell Biology Cell Cycle Kinases/Phosphatases Phosphatases
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RecombinantRabMAb

Recombinant HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (1)

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Western blot - HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)
  • HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EP1511Y] to PPP1CA + PPP1CB
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Conjugation: HRP

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Overview

  • Product name

    HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y]
    See all PPP1CA + PPP1CB primary antibodies
  • Description

    HRP Rabbit monoclonal [EP1511Y] to PPP1CA + PPP1CB
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa whole cell and Mouse Brain and Rat Brain tissue lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin 300 Solution
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1511Y
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Phosphatases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Phosphatases
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Phospatases
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Other
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)
    • Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab52619)
  • Isotype control

    • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab211372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).
Notes
WB
1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).

Target

  • Cellular localization

    Cytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus.
  • Database links

    • Entrez Gene: 5499 Human
    • Entrez Gene: 5500 Human
    • Entrez Gene: 19045 Mouse
    • Entrez Gene: 19046 Mouse
    • Entrez Gene: 24668 Rat
    • Entrez Gene: 25594 Rat
    • Omim: 176875 Human
    • Omim: 600590 Human
    • SwissProt: P62136 Human
    • SwissProt: P62140 Human
    • SwissProt: P62137 Mouse
    • SwissProt: P62141 Mouse
    • SwissProt: P62138 Rat
    • SwissProt: P62142 Rat
    see all
  • Alternative names

    • PP-1A antibody
    • PP-1B antibody
    • PP1A antibody
    • PP1alpha antibody
    • PP1B antibody
    • PP1beta antibody
    • PPP1A antibody
    • PPP1CA antibody
    • PPP1CB antibody
    • PPP1CD antibody
    • protein phosphatase 1 catalytic subunit alpha isozyme antibody
    • protein phosphatase 1 catalytic subunit beta isoform antibody
    • protein phosphatase 1 catalytic subunit delta isoform antibody
    • serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit antibody
    • Serine/threonine-protein phosphatase PP1-alpha catalytic subunit antibody
    • Serine/threonine-protein phosphatase PP1-beta catalytic subunit antibody
    see all

Images

  • Western blot - HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)
    Western blot - HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)
    All lanes : HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372) at 1/5000 dilution

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : HeLa whole cell lysate (ab150035)

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 37 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab211372 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)
    HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (1)

Publishing research using ab211372? Please let us know so that we can cite the reference in this datasheet.

ab211372 has been referenced in 1 publication.

  • Hu Z  et al. Protein phosphatase 1 catalyzes HBV core protein dephosphorylation and is co-packaged with viral pregenomic RNA into nucleocapsids. PLoS Pathog 16:e1008669 (2020). PubMed: 32702076

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