RecombinantRabMAb

Recombinant HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)

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Western blot - HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)
  • HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EP1511Y] to PPP1CA + PPP1CB
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y]
    See all PPP1CA + PPP1CB primary antibodies

  • Description

    HRP Rabbit monoclonal [EP1511Y] to PPP1CA + PPP1CB

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human PPP1CA + PPP1CB aa 200-300. The exact sequence is proprietary.
    Database link: P62140

  • Positive control

    • WB: HeLa whole cell and Mouse Brain and Rat Brain tissue lysates.

  • General notes

     

     

     This product was previously labelled as Protein Phosphatase 1 beta, PPP1CA + 1CB

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab211372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).

Target

Images

  • Western blot - HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)
    Western blot - HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)
    All lanes : HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372) at 1/5000 dilution

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : HeLa whole cell lysate (ab150035)

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 37 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab211372 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)
    HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)

References (1)

Publishing research using ab211372? Please let us know so that we can cite the reference in this datasheet.

ab211372 has been referenced in 1 publication.

  • Hu Z  et al. Protein phosphatase 1 catalyzes HBV core protein dephosphorylation and is co-packaged with viral pregenomic RNA into nucleocapsids. PLoS Pathog 16:e1008669 (2020). PubMed: 32702076

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