HRP Anti-proBNP antibody [13G12] (ab14712)

Submit a review Q&A (1)References (1)

Key features and details

  • HRP Mouse monoclonal [13G12] to proBNP
  • Reacts with: Human
  • Conjugation: HRP
  • Isotype: IgG2a

Overview

  • Product name

    HRP Anti-proBNP antibody [13G12]
    See all proBNP primary antibodies

  • Description

    HRP Mouse monoclonal [13G12] to proBNP

  • Host species

    Mouse

  • Conjugation

    HRP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide:

    ETSGLQEQRNHLQGK

    , corresponding to N terminal amino acids 13-27 of Human proBNP.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Proclin 300
    Constituents: 0.8% Sodium chloride, 0.02% Potassium chloride, 0.024% Monobasic dihydrogen potassium phosphate, 0.18% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Chromatography on protein A Sepharose

  • Clonality

    Monoclonal

  • Clone number

    13G12

  • Myeloma

    Sp2/0

  • Isotype

    IgG2a

  • Research areas

Target

  • Function

    Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

  • Tissue specificity

    Brain and also in atria, but at much lower levels than ANP.

  • Sequence similarities

    Belongs to the natriuretic peptide family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • ANFB_HUMAN antibody
      • BNP antibody
      • BNP(1-32) antibody
      • BNP(5-29) antibody
      • BNP-32 antibody
      • Brain type natriuretic peptide antibody
      • Gamma brain natriuretic peptide antibody
      • Gamma-brain natriuretic peptide antibody
      • Natriuretic peptide precursor B antibody
      • nppb antibody
      • NT proBNP antibody
      see all

    References (1)

    Publishing research using ab14712? Please let us know so that we can cite the reference in this datasheet.

    ab14712 has been referenced in 1 publication.

    • Kim YS  et al. High-level production of N-terminal pro-brain natriuretic peptide, as a calibrant of heart failure diagnosis, in Escherichia coli. Appl Microbiol Biotechnol 103:4779-4788 (2019). PubMed: 31041470

    Customer reviews and Q&As

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    Question

    Inquiry: Dear Sir/Madam, I am looking for antibodies directed at or cross-reactive with rat NT-pro-BNP. Preferably monoclonal and source from any species. There are multiple monoclonal available on your site, but directed to human. Do you have antibodies that are crossreactive to rat. Best Regards,

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    Answer

    Thank you very much for your inquiry.

    I have to confirm that do not have any pro-BNP antibodies that are tested and guaranteed for rat.

    I checked the immunogens for the available pro-BNP antibodies and unfortunately I cannot recommend any of for testing in rat.

    We usually expect an cross-reaction when the homology is 85% and higher. But the different regions that the immunogens are taken from only show 62% and less homology:

    AA 1-12 no homology
    AA 13-27 58%
    AA 28-45 62%
    AA 61-76 no homology.

    I am sorry I could not be of more help on thisoccasion and wish you goo dluck with your research.

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