HRP Anti-proBNP antibody [24E11] (ab13123)
![Western blot - HRP Anti-proBNP antibody [24E11] (ab13123)](https://www.abcam.com/ps/products/13/ab13123/Images/ab13111_1.jpg "Western blot - HRP Anti-proBNP antibody [24E11] (ab13123)")
Key features and details
- HRP Mouse monoclonal [24E11] to proBNP
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-proBNP antibody [24E11]
See all proBNP primary antibodies -
Description
HRP Mouse monoclonal [24E11] to proBNP -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
RGHRKMVLYTLRAPR
, corresponding to amino acids 61 - 76 of Human proBNP. -
General notes
Immunoglobulin concentrations vary from batch to batch. Please contact technical services for the latest information.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Proclin 300
Constituents: 0.8% Sodium chloride, 0.02% Potassium chloride, 0.024% Monobasic dihydrogen potassium phosphate, 0.18% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Chromatography on protein A Sepharose Purified from TCS -
Clonality
Monoclonal -
Clone number
24E11 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Positive Controls
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Related Products
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-
sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab13123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 13 kDa. |
Target
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Function
Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. -
Tissue specificity
Brain and also in atria, but at much lower levels than ANP. -
Sequence similarities
Belongs to the natriuretic peptide family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 4879 Human
- Omim: 600295 Human
- SwissProt: P16860 Human
- Unigene: 219140 Human
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Alternative names
- ANFB_HUMAN antibody
- BNP antibody
- BNP(1-32) antibody
see all
Images
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Western blot - HRP Anti-proBNP antibody [24E11] (ab13123)WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11]. WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11].
References (1)
ab13123 has been referenced in 1 publication.
- Kim YS et al. High-level production of N-terminal pro-brain natriuretic peptide, as a calibrant of heart failure diagnosis, in Escherichia coli. Appl Microbiol Biotechnol 103:4779-4788 (2019). PubMed: 31041470
