HRP Anti-proBNP antibody [24E11] (ab13123)

Submit a review Q&A (1)References (1)
Western blot - HRP Anti-proBNP antibody [24E11] (ab13123)

    Key features and details

    • HRP Mouse monoclonal [24E11] to proBNP
    • Suitable for: WB
    • Conjugation: HRP
    • Isotype: IgG

    Overview

    • Product name

      HRP Anti-proBNP antibody [24E11]
      See all proBNP primary antibodies

    • Description

      HRP Mouse monoclonal [24E11] to proBNP

    • Host species

      Mouse

    • Conjugation

      HRP

    • Tested applications

      Suitable for: WBmore details

    • Immunogen

      Synthetic peptide:

      RGHRKMVLYTLRAPR

      , corresponding to amino acids 61 - 76 of Human proBNP.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • General notes

      Immunoglobulin concentrations vary from batch to batch. Please contact technical services for the latest information.

       

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 7.40
      Preservative: 0.05% Proclin 300
      Constituents: 0.8% Sodium chloride, 0.02% Potassium chloride, 0.024% Monobasic dihydrogen potassium phosphate, 0.18% Dibasic monohydrogen sodium phosphate
    • Concentration information loading...

    • Purity

      Protein A purified

    • Purification notes

      Chromatography on protein A Sepharose Purified from TCS

    • Clonality

      Monoclonal

    • Clone number

      24E11

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab13123 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use at an assay dependent concentration. Detects a band of approximately 13 kDa.
    Notes
    WB
    Use at an assay dependent concentration. Detects a band of approximately 13 kDa.

    Target

    • Function

      Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

    • Tissue specificity

      Brain and also in atria, but at much lower levels than ANP.

    • Sequence similarities

      Belongs to the natriuretic peptide family.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Alternative names

      • ANFB_HUMAN antibody
      • BNP antibody
      • BNP(1-32) antibody
      • BNP(5-29) antibody
      • BNP-32 antibody
      • Brain type natriuretic peptide antibody
      • Gamma brain natriuretic peptide antibody
      • Gamma-brain natriuretic peptide antibody
      • Natriuretic peptide precursor B antibody
      • nppb antibody
      • NT proBNP antibody
      see all

    Images

    • Western blot - HRP Anti-proBNP antibody [24E11] (ab13123)
      Western blot - HRP Anti-proBNP antibody [24E11] (ab13123)
      WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11]. WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11].

    References (1)

    Publishing research using ab13123? Please let us know so that we can cite the reference in this datasheet.

    ab13123 has been referenced in 1 publication.

    • Kim YS  et al. High-level production of N-terminal pro-brain natriuretic peptide, as a calibrant of heart failure diagnosis, in Escherichia coli. Appl Microbiol Biotechnol 103:4779-4788 (2019). PubMed: 31041470

    Customer reviews and Q&As

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    Question

    Inquiry: Dear Sir/Madam, I am looking for antibodies directed at or cross-reactive with rat NT-pro-BNP. Preferably monoclonal and source from any species. There are multiple monoclonal available on your site, but directed to human. Do you have antibodies that are crossreactive to rat. Best Regards,

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    Answer

    Thank you very much for your inquiry.

    I have to confirm that do not have any pro-BNP antibodies that are tested and guaranteed for rat.

    I checked the immunogens for the available pro-BNP antibodies and unfortunately I cannot recommend any of for testing in rat.

    We usually expect an cross-reaction when the homology is 85% and higher. But the different regions that the immunogens are taken from only show 62% and less homology:

    AA 1-12 no homology
    AA 13-27 58%
    AA 28-45 62%
    AA 61-76 no homology.

    I am sorry I could not be of more help on thisoccasion and wish you goo dluck with your research.

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