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    hrp-probnp-antibody-24e11-ab13123.pdf

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HRP Anti-proBNP antibody [24E11] (ab13123)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (1)

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Western blot - HRP Anti-proBNP antibody [24E11] (ab13123)

    Key features and details

    • HRP Mouse monoclonal [24E11] to proBNP
    • Suitable for: WB
    • Reacts with: Human
    • Conjugation: HRP
    • Isotype: IgG

    Overview

    • Product name

      HRP Anti-proBNP antibody [24E11]
      See all proBNP primary antibodies
    • Description

      HRP Mouse monoclonal [24E11] to proBNP
    • Host species

      Mouse
    • Conjugation

      HRP
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide:

      RGHRKMVLYTLRAPR

      , corresponding to amino acids 61 - 76 of Human proBNP.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

      Immunoglobulin concentrations vary from batch to batch. Please contact technical services for the latest information.

       

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.40
      Preservative: 0.05% Proclin 300
      Constituents: 0.8% Sodium chloride, 0.02% Potassium chloride, 0.024% Monobasic dihydrogen potassium phosphate, 0.18% Dibasic monohydrogen sodium phosphate
    • Concentration information loading...
    • Purity

      Protein A purified
    • Purification notes

      Chromatography on protein A Sepharose Purified from TCS
    • Clonality

      Monoclonal
    • Clone number

      24E11
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurotransmitter
      • Neuropeptides
      • Hormones
      • Cardiovascular
      • Heart
      • Hypertrophy
      • Hormones
      • Cardiovascular
      • Heart
      • Hypertrophy
      • Other

    Associated products

    • Alternative Versions

      • Anti-proBNP antibody [24E11] (ab218141)
    • Positive Controls

      • Recombinant Human BNP protein (ab87200)
    • Related Products

      • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
      • TMB ELISA Substrate (High Sensitivity) (ab171523)
      • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
      • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
      • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
      • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
      • 450 nm Stop Solution for TMB Substrate (ab171529)
      • 650 nm Stop Solution for TMB Substrate (ab171531)
      • Immunoassay Blocking Buffer (ab171534)
      • Immunoassay Blocking (BSA Free) (ab171535)
    • sELISA pair antibody

      • Anti-NT-proBNP antibody [15F11] (ab13115)

    Applications

    Our Abpromise guarantee covers the use of ab13123 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent concentration. Detects a band of approximately 13 kDa.

    Target

    • Function

      Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
    • Tissue specificity

      Brain and also in atria, but at much lower levels than ANP.
    • Sequence similarities

      Belongs to the natriuretic peptide family.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P16860 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4879 Human
      • Omim: 600295 Human
      • SwissProt: P16860 Human
      • Unigene: 219140 Human
      • Alternative names

        • ANFB_HUMAN antibody
        • BNP antibody
        • BNP(1-32) antibody
        • BNP(5-29) antibody
        • BNP-32 antibody
        • Brain type natriuretic peptide antibody
        • Gamma brain natriuretic peptide antibody
        • Gamma-brain natriuretic peptide antibody
        • Natriuretic peptide precursor B antibody
        • nppb antibody
        • NT proBNP antibody
        see all

      Images

      • Western blot - HRP Anti-proBNP antibody [24E11] (ab13123)
        Western blot - HRP Anti-proBNP antibody [24E11] (ab13123)
        WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11]. WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11].

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab13123? Please let us know so that we can cite the reference in this datasheet.

      ab13123 has been referenced in 1 publication.

      • Kim YS  et al. High-level production of N-terminal pro-brain natriuretic peptide, as a calibrant of heart failure diagnosis, in Escherichia coli. Appl Microbiol Biotechnol 103:4779-4788 (2019). PubMed: 31041470

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Inquiry: Dear Sir/Madam, I am looking for antibodies directed at or cross-reactive with rat NT-pro-BNP. Preferably monoclonal and source from any species. There are multiple monoclonal available on your site, but directed to human. Do you have antibodies that are crossreactive to rat. Best Regards,

      Read More

      Abcam community

      Verified customer

      Asked on May 11 2012

      Answer

      Thank you very much for your inquiry.

      I have to confirm that do not have any pro-BNP antibodies that are tested and guaranteed for rat.

      I checked the immunogens for the available pro-BNP antibodies and unfortunately I cannot recommend any of for testing in rat.

      We usually expect an cross-reaction when the homology is 85% and higher. But the different regions that the immunogens are taken from only show 62% and less homology:

      AA 1-12 no homology
      AA 13-27 58%
      AA 28-45 62%
      AA 61-76 no homology.

      I am sorry I could not be of more help on thisoccasion and wish you goo dluck with your research.

      Read More

      Abcam Scientific Support

      Answered on May 11 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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