Recombinant HRP Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (ab203462)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR8030(B)] to Prostaglandin E Receptor EP2/PTGER2
- Suitable for: WB
- Reacts with: Mouse, Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)]
See all Prostaglandin E Receptor EP2/PTGER2 primary antibodies -
Description
HRP Rabbit monoclonal [EPR8030(B)] to Prostaglandin E Receptor EP2/PTGER2 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Placenta (Human), Lung (Human), JAR (Human Choriocarcinoma), WI38 (Human lung fibroblast cell line) and Liver (Mouse) Whole Cell Lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8030(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (ab167171)
- Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] - BSA and Azide free (ab236779)
- Alexa Fluor® 488 Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (ab246777)
- Alexa Fluor® 647 Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (ab246778)
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 40 kDa).
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Notes |
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WB
1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 40 kDa). |
Target
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Function
Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle. -
Tissue specificity
Placenta and lung. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 5732 Human
- Entrez Gene: 19217 Mouse
- Entrez Gene: 100360765 Rat
- Entrez Gene: 81752 Rat
- Omim: 176804 Human
- SwissProt: P43116 Human
- SwissProt: Q62053 Mouse
- SwissProt: Q62928 Rat
see all -
Alternative names
- EP2 antibody
- PE2R2_HUMAN antibody
- PGE receptor EP2 subtype antibody
see all
Images
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All lanes : HRP Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (ab203462) at 1/5000 dilution
Lane 1 : Placenta (Human) Tissue Lysate - adult normal tissue
Lane 2 : Lung (Human) Whole Cell Lysate - fetal normal tissue
Lane 3 : JAR (Human Choriocarcinoma) Whole Cell Lysate
Lane 4 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate
Lane 5 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab203462 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab203462 has not yet been referenced specifically in any publications.