Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP1841Y] to PTP1B
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Product nameHRP Anti-PTP1B antibody [EP1841Y]
See all PTP1B primary antibodies
DescriptionHRP Rabbit monoclonal [EP1841Y] to PTP1B
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PTP1B aa 350 to the C-terminus. The exact sequence is proprietary.
Database link: P18031
- WB: HeLa, HepG2, Jurkat and wildtype HAP1 whole cell lysates.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab207635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 50 kDa).|
FunctionMay play an important role in CKII- and p60c-src-induced signal transduction cascades.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
Contains 1 tyrosine-protein phosphatase domain.
modificationsOxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- PTP1B antibody
- Non receptor tyrosine phosphatase 1 antibody
- Protein phosphotyrosylphosphatase 1B antibody
All lanes : HRP Anti-PTP1B antibody [EP1841Y] (ab207635) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 4 : Wild-type HAP1 cell lysate at 20 µg
Lane 5 : PTPB1 knockout HAP1 cell lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab207635 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab207635 has not yet been referenced specifically in any publications.