HRP Anti-R Phycoerythrin/rpeA antibody (ab34727)
Key features and details
- HRP Goat polyclonal to R Phycoerythrin/rpeA
- Suitable for: WB, Dot blot, ELISA, Electron Microscopy
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-R Phycoerythrin/rpeA antibody
See all R Phycoerythrin/rpeA primary antibodies -
Description
HRP Goat polyclonal to R Phycoerythrin/rpeA -
Host species
Goat -
Conjugation
HRP -
Specificity
This antibody cross reacts with B Phycoerythrin. -
Tested applications
Suitable for: WB, Dot blot, ELISA, Electron Microscopymore details -
Species reactivity
Reacts with: Red Algae -
Immunogen
Full length protein corresponding to R Phycoerythrin/rpeA. Corresponds to the seaweed Gracilaria
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purification notes
This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using a R Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | ||
Dot blot | ||
ELISA | ||
Electron Microscopy |
Notes |
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Immunoperoxidase Electron Microscopy: Use at an assay dependent dilution.
ELISA: 1/2,000 - 1/10,000.
WB: 1/500 - 1/2,000. Predicted molecular weight: 240 kDa.
Not yet tested in other applications but thought to be suitable for other peroxidase-antibody based enzymatic assays.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents. -
Cellular localization
Plastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome. -
Alternative names
- R phycoerythrin alpha subunit antibody
- R phycoerythrin beta subunit antibody
- rpeA antibody
- rpeB antibody
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab34727 has not yet been referenced specifically in any publications.