HRP Anti-R Phycoerythrin/rpeA antibody (ab34727)

Submit a review Q&A (5)

Key features and details

  • HRP Goat polyclonal to R Phycoerythrin/rpeA
  • Suitable for: WB, Dot blot, ELISA, Electron Microscopy
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG

Overview

  • Product name

    HRP Anti-R Phycoerythrin/rpeA antibody
    See all R Phycoerythrin/rpeA primary antibodies

  • Description

    HRP Goat polyclonal to R Phycoerythrin/rpeA

  • Host species

    Goat

  • Conjugation

    HRP

  • Specificity

    This antibody cross reacts with B Phycoerythrin.

  • Tested applications

    Suitable for: WB, Dot blot, ELISA, Electron Microscopymore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Full length protein corresponding to R Phycoerythrin/rpeA. Corresponds to the seaweed Gracilaria

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...

  • Purification notes

    This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using a R Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab34727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Dot blot
ELISA
Electron Microscopy
Notes
Application notes
Dot blot: Use at an assay dependent dilution.
Immunoperoxidase Electron Microscopy: Use at an assay dependent dilution.
ELISA: 1/2,000 - 1/10,000.
WB: 1/500 - 1/2,000. Predicted molecular weight: 240 kDa.

Not yet tested in other applications but thought to be suitable for other peroxidase-antibody based enzymatic assays.
Optimal dilutions/concentrations should be determined by the end user.

Target

  • Relevance

    Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.

  • Cellular localization

    Plastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome.

  • Alternative names

    • R phycoerythrin alpha subunit antibody
    • R phycoerythrin beta subunit antibody
    • rpeA antibody
    • rpeB antibody

References (0)

Publishing research using ab34727? Please let us know so that we can cite the reference in this datasheet.

ab34727 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Question

I found the order for the last PE-HRP we bought. The confirmation email says, "Abcamorder (ref:ORDER #***) despatch confirmation [CCE2683621]". I don't see a lot # associated with the order forms but perhaps you have a record? Is there any more info you need from me? Sorry for such a delay.

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Answer

Thank you for sending this information.

I've found the order information and looked up the lot number, so this is very helpfulfor our internal investigation into the problem with this antibody. You should be receiving the new vial of ab34727 tomorrow, so please keep me updated about the results using this replacement.

I apologize again for all of the inconvenience, but please let me know if there is anything else that we can do for you.

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Question

Yes, sorry for the delayed response. I will have to spend some time looking through our records to find the last time we bought anti-PE-HRP from you, and even then I am not sure I'll be able to get the lot number. Unfortunately we threw away the old vial already.

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Answer

Thanks for your reply.

Please take your time, it's no problem. Weare preparing new aliquots of the antibody and will be sending you another vial on the order ***. I expect this to arrive late next week.

I apologize again for the inconvenience and for the delay, but please keep me updated about how this new antibody works for you, and let me know if there is anything else you might need from us.

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Question

Thanks for the message and your help. Yesterday we tested the new anti-PE-HRP in an exact repeat experiment of one we performed last week,using the same cells, procedures, etc. The new anti-PE-HRP that contains the brown precipitate did not work at all in this assay. I could not do a side-by-side, same day comparison experiment since we are out of the old lot, but I am concerned there is something wrong with the new anti-PE-HRP. Do you think we could get a batch of the newlot ofanti-PE-HEP to test?

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Answer

Thank you for your reply and for keeping me udpated.

I'm sorry for the delay, but all of our shipment-ready stock does contain the orange precipitate. I'm currently working with our supply chain to get some vials without this problem and hope to resolve this promptly. I realize that your work is being held up, and I again apologize for this.

In the meantime, could you forward me some additional details about the assay that you're performing? I didn't realize that you had previously used this product successfully, and I just want to make sure that the problem is due directly to the precipitate and not to a different quality issue of the current lot.

I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

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Question

Thanks for the message and your help. Yesterday we tested the new anti-PE-HRP in an exact repeat experiment of one we performed last week,using the same cells, procedures, etc. The new anti-PE-HRP that contains the brown precipitate did not work at all in this assay. I could not do a side-by-side, same day comparison experiment since we are out of the old lot, but I am concerned there is something wrong with the new anti-PE-HRP. Do you think we could get a batch of the newlot ofanti-PE-HEP to test?

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Answer

Thanks for keeping me updated, and I apologize that there have been so many problems with this antibody.

I am checking with the lab to see if there's another lotto send, and I will be happy to send a vial if so.

I didn't realize when we spoke that you've used a different vial of this antibody last week and it worked in your assay. When was the working vial ordered, and what lot is it from? For our records, could you tell me what kind of assay you're performing?

I will be back in touch tomorrow once I hear from the lab. Please feel free to give me a call if you'd like to speak to me or send a reply to this email if you have any further questions or need anything else from me.

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Question

Here are the two vials of anti-PE-HRP that have some sort of stuff in them. It became apparent after spinning down the tube for a few seconds. Let me know what you find out!

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Answer

Thank you for your call today and for sending the images, and I apologize for the trouble with this antibody.

I just left a voicemail for you, but I wanted to be in touch via email as well. The scientists in the lab did say that they expect the antibody to perform as usual, and the precipitate is just from the HRP conjugation. I inspected the last vial that we have in stock, and it also contains the orange pellet, so if the antibody does not give the expected results, I will send a free of charge vial from a new lot.

Please keep me updated about the results with this antibody, and let me know if you have any questions or if there is anything else that we can do for you.

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