RecombinantRabMAb

Recombinant HRP Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab199205)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab199205)
  • Western blot - HRP Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab199205)
  • Western blot - HRP Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab199205)
  • HRP Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab199205)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR3042] to Rab4 - Early Endosome Marker
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-Rab4 antibody [EPR3042] - Early Endosome Marker
    See all Rab4 primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR3042] to Rab4 - Early Endosome Marker

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human Rab4 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: MCF-7 Whole Cell and Human Fetal Brain tissue lysates. IHC-P: FFPE human stomach (normal) tissue sections.

  • General notes

    Alternative versions available:
    Anti-Rab4 antibody - Early Endosome Marker [EPR3042] (ab108974) - Knockout validated 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab199205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab199205)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab199205)

    IHC image of Rab4 staining in a section of formalin-fixed paraffin-embedded normal human stomach tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab199205 at 1/100 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - HRP Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab199205)
    Western blot - HRP Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab199205)
    HRP Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab199205) at 1/5000 dilution + MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 24 kDa


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199205 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • Western blot - HRP Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab199205)
    Western blot - HRP Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab199205)
    HRP Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab199205) at 1/5000 dilution + Fetal Liver (Human) Normal Tissue Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 24 kDa


    Exposure time: 10 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199205 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • HRP Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab199205)
    HRP Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab199205)

References (0)

Publishing research using ab199205? Please let us know so that we can cite the reference in this datasheet.

ab199205 has not yet been referenced specifically in any publications.

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