Recombinant HRP Anti-RAB5C/RABL antibody [EPR17321] (ab209475)
![Western blot - HRP Anti-RAB5C/RABL antibody [EPR17321] (ab209475)](https://www.abcam.com/ps/products/209/ab209475/Images/ab209475-265757-anti-rab5c-antibody-epr17321-hrp-western-blot.jpg "Western blot - HRP Anti-RAB5C/RABL antibody [EPR17321] (ab209475)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR17321] to RAB5C/RABL
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-RAB5C/RABL antibody [EPR17321]
See all RAB5C/RABL primary antibodies -
Description
HRP Rabbit monoclonal [EPR17321] to RAB5C/RABL -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human RAB5C/RABL aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P51148 -
Positive control
- WB: HEK293, HepG2 cell lysates, Human Fetal Heart tissue lysate
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General notes
This product was previously labelled as RAB5C
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17321 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab209475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). |
Target
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Function
Protein transport. Probably involved in vesicular traffic. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Cell membrane. Early endosome membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 5878 Human
- Omim: 604037 Human
- SwissProt: P51148 Human
- Unigene: 650382 Human
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Alternative names
- L1880 antibody
- RAB, member of RAS oncogene family like antibody
- RAB5C antibody
see all
Images
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Western blot - HRP Anti-RAB5C/RABL antibody [EPR17321] (ab209475)All lanes : HRP Anti-RAB5C/RABL antibody [EPR17321] (ab209475) at 1/5000 dilution
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Brain (Human) Tissue Lysate - fetal normal tissue
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab209475 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-RAB5C/RABL antibody [EPR17321] (ab209475)](https://www.abcam.com/ps/products/209/ab209475/Images/ab209475-2-benefits-of-recombinant-antibodies.png)
HRP Anti-RAB5C/RABL antibody [EPR17321] (ab209475)
References (0)
ab209475 has not yet been referenced specifically in any publications.
