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Secondary antibodies anti-Rabbit IgG Enzyme HRP
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RecombinantRabMAb

Recombinant HRP Anti-Rabbit IgG antibody [SP137] (ab270144)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rabbit IgG antibody [SP137] (ab270144)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rabbit IgG antibody [SP137] (ab270144)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rabbit IgG antibody [SP137] (ab270144)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [SP137] to Rabbit IgG
  • Suitable for: IHC-P
  • Reacts with: Rat, Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-Rabbit IgG antibody [SP137]
    See all Rabbit IgG primary antibodies
  • Description

    HRP Rabbit monoclonal [SP137] to Rabbit IgG
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    Human
    See all applications and species data
  • Immunogen

    Chemical/ Small Molecule corresponding to Rabbit IgG.

  • Positive control

    • IHC-P: Human normal tonsil tissue. Mouse and rat normal spleen tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68.88% PBS
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Clonality

    Monoclonal
  • Clone number

    SP137
  • Isotype

    IgG
  • Research areas

    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Enzyme
    • HRP
    • Isotype/Loading Controls
    • Isotype Controls
    • Rabbit

Associated products

  • Alternative Versions

    • Rabbit IgG, monoclonal [SP137] - isotype control (ab125938)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Rat
Human
All applications
Mouse
Application Abreviews Notes
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Relevance

    IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
  • Cellular localization

    Secreted
  • Alternative names

    • Ig gamma chain C region antibody
    • Ig kappa-b4 chain C region antibody
    • Ig kappa-b5 chain C region antibody
    • Ig kappa-b9 chain C region antibody
    • Ig lambda chain C region antibody
    • K-BAS antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rabbit IgG antibody [SP137] (ab270144)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rabbit IgG antibody [SP137] (ab270144)

    IHC image of IgG Isotype control staining in a section of formalin-fixed paraffin-embedded normal human tonsil*, performed on a Leica BOND®.

    The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab270144, 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rabbit IgG antibody [SP137] (ab270144)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rabbit IgG antibody [SP137] (ab270144)

    IHC image of IgG Isotype control staining in a section of formalin-fixed paraffin-embedded normal mouse spleen*, performed on a Leica BOND®.

    The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab270144, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rabbit IgG antibody [SP137] (ab270144)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rabbit IgG antibody [SP137] (ab270144)

    IHC image of IgG Isotype control staining in a section of formalin-fixed paraffin-embedded normal rat spleen*, performed on a Leica BOND®.

    The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab270144, 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab270144? Please let us know so that we can cite the reference in this datasheet.

    ab270144 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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