Key features and details
- HRP Mouse monoclonal [2C4] to Rainbow Ladder
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG1
Product nameHRP Anti-Rainbow Ladder antibody [2C4]
DescriptionHRP Mouse monoclonal [2C4] to Rainbow Ladder
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Species independent
Other Immunogen Type corresponding to Rainbow Ladder. Remazol dye stained proteins, prestained multi-color protein markers.
Ab231759 this monoclonal detects multi-color pre-stained protein markers (specifically Remazol dye stained proteins). It allows the visualisation of pre-stained protein ladders in combination with the bands of interest without needing to merge images or manually mark film (PubMed ID: 27531616). The antibody is conjugated to HRP to permit direct visualisation by chemiluminescence.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab231759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
All lanes : HRP Anti-Rainbow Ladder antibody [2C4] (ab231759) at 1/5000 dilution
Lane 1 : Chameleon Marker
Lane 2 : Thermo Scientific Pageruler Plus
Lane 3 : All Blue BioRad
Lysates/proteins at 1 µl per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 30 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab231759 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231759 has not yet been referenced specifically in any publications.