Recombinant HRP Anti-RanGAP1 antibody [EPR3295] (ab205449)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-RanGAP1 antibody [EPR3295] (ab205449)](https://www.abcam.com/ps/products/205/ab205449/Images/ab205449-267182-IHCAP00233671.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-RanGAP1 antibody [EPR3295] (ab205449)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR3295] to RanGAP1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-RanGAP1 antibody [EPR3295]
See all RanGAP1 primary antibodies -
Description
HRP Rabbit monoclonal [EPR3295] to RanGAP1 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. within Human RanGAP1 aa 1-100. The exact sequence is proprietary.
Database link: P46060 -
Positive control
- WB: HeLa cell lysate. IHC-P: normal human testis tissue sections
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EPR3295 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab205449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/5000. Detects a band of approximately 70 kDa (predicted molecular weight: 64 kDa). |
Target
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Function
GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state. -
Tissue specificity
Highly expressed in brain, thymus and testis. -
Sequence similarities
Belongs to the RNA1 family.
Contains 6 LRR (leucine-rich) repeats. -
Post-translational
modificationsPhosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis.
Sumoylated with SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9. -
Cellular localization
Cytoplasm. Nucleus membrane. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole. Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase. Mitotic location also requires sumoylation. - Information by UniProt
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Database links
- Entrez Gene: 5905 Human
- Entrez Gene: 19387 Mouse
- Entrez Gene: 362965 Rat
- Omim: 602362 Human
- SwissProt: P46060 Human
- SwissProt: P46061 Mouse
- Unigene: 183800 Human
- Unigene: 270975 Mouse
see all -
Alternative names
- Fug 1 antibody
- Fug1 antibody
- GTPase-activating protein, RAN, 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-RanGAP1 antibody [EPR3295] (ab205449)
IHC image of RanGAP1 staining in a section of formalin-fixed paraffin-embedded normal human testis*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab205449, 1/1000 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - HRP Anti-RanGAP1 antibody [EPR3295] (ab205449)](https://www.abcam.com/ps/products/205/ab205449/Images/ab205449-265704-WBab2054491.jpg)
Western blot - HRP Anti-RanGAP1 antibody [EPR3295] (ab205449)HRP Anti-RanGAP1 antibody [EPR3295] (ab205449) at 1/5000 dilution + SH-SY5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205449 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-RanGAP1 antibody [EPR3295] (ab205449)](https://www.abcam.com/ps/products/205/ab205449/Images/ab205449-4-benefits-of-recombinant-antibodies.png)
HRP Anti-RanGAP1 antibody [EPR3295] (ab205449)
Protocols
References (0)
ab205449 has not yet been referenced specifically in any publications.
