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    hrp-ras-antibody-epr3255-ab199557.pdf

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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family
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RecombinantRabMAb

Recombinant HRP Anti-Ras antibody [EPR3255] (ab199557)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - HRP Anti-Ras antibody [EPR3255] (ab199557)
  • Western blot - HRP Anti-Ras antibody [EPR3255] (ab199557)
  • HRP Anti-Ras antibody [EPR3255] (ab199557)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR3255] to Ras
  • Suitable for: WB
  • Reacts with: Human
  • Conjugation: HRP

You may also be interested in

Protein
Product image
Recombinant Human Ras protein (ab61239)

View more associated products

Overview

  • Product name

    HRP Anti-Ras antibody [EPR3255]
    See all Ras primary antibodies
  • Description

    HRP Rabbit monoclonal [EPR3255] to Ras
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Ras aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HEK293 and SHSY-5Y whole cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: 30% Glycerol, PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3255
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Cancer
    • Signal transduction
    • G protein signaling
    • Small G proteins
    • Ras family
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Signal transducers
    • Cancer
    • Tumor biomarkers
    • Oncoproteins

Associated products

  • Alternative Versions

    • Anti-Ras antibody [EPR3255] (ab108602)
    • Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)
  • Isotype control

    • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)
  • Recombinant Protein

    • Recombinant Human Ras protein (ab61239)
  • Related Products

    • ECL Substrate Kit (High Sensitivity) (ab133406)

Applications

Our Abpromise guarantee covers the use of ab199557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

Target

  • Function

    Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
  • Involvement in disease

    Defects in HRAS are the cause of faciocutaneoskeletal syndrome (FCSS) [MIM:218040]. A rare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities (typically pulmonic stenosis, hypertrophic cardiomyopathy and/or atrial tachycardia), tumor predisposition, skin and musculoskeletal abnormalities.
    Defects in HRAS are the cause of congenital myopathy with excess of muscle spindles (CMEMS) [MIM:218040]. CMEMS is a variant of Costello syndrome.
    Defects in HRAS may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms.
    Note=Mutations which change positions 12, 13 or 61 activate the potential of HRAS to transform cultured cells and are implicated in a variety of human tumors.
    Defects in HRAS are a cause of susceptibility to bladder cancer (BLC) [MIM:109800]. A malignancy originating in tissues of the urinary bladder. It often presents with multiple tumors appearing at different times and at different sites in the bladder. Most bladder cancers are transitional cell carcinomas. They begin in cells that normally make up the inner lining of the bladder. Other types of bladder cancer include squamous cell carcinoma (cancer that begins in thin, flat cells) and adenocarcinoma (cancer that begins in cells that make and release mucus and other fluids). Bladder cancer is a complex disorder with both genetic and environmental influences.
    Note=Defects in HRAS are the cause of oral squamous cell carcinoma (OSCC).
  • Sequence similarities

    Belongs to the small GTPase superfamily. Ras family.
  • Post-translational
    modifications

    Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
    S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation.
  • Cellular localization

    Cell membrane. Golgi apparatus membrane. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus.
  • Target information above from: UniProt accession P01112 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3265 Human
    • Entrez Gene: 3845 Human
    • Entrez Gene: 4893 Human
    • Entrez Gene: 15461 Mouse
    • Entrez Gene: 16653 Mouse
    • Entrez Gene: 18176 Mouse
    • Entrez Gene: 24525 Rat
    • Entrez Gene: 24605 Rat
    • Entrez Gene: 293621 Rat
    • Omim: 164790 Human
    • Omim: 190020 Human
    • Omim: 190070 Human
    • SwissProt: P01111 Human
    • SwissProt: P01112 Human
    • SwissProt: P01116 Human
    • SwissProt: P08556 Mouse
    • SwissProt: P32883 Mouse
    • SwissProt: Q61411 Mouse
    • SwissProt: P08644 Rat
    • SwissProt: P20171 Rat
    • SwissProt: Q04970 Rat
    • Unigene: 37003 Human
    • Unigene: 486502 Human
    • Unigene: 505033 Human
    • Unigene: 334313 Mouse
    • Unigene: 102180 Rat
    see all
  • Alternative names

    • C BAS/HAS antibody
    • C HA RAS1 antibody
    • C-BAS/HAS antibody
    • c-H-ras antibody
    • C-HA-RAS1 antibody
    • CTLO antibody
    • GTPase HRas antibody
    • GTPase KRas antibody
    • GTPase NRas antibody
    • H ras antibody
    • H RASIDX antibody
    • H-Ras-1 antibody
    • H-RASIDX antibody
    • Ha-Ras antibody
    • HAMSV antibody
    • HRAS antibody
    • HRAS1 antibody
    • K ras antibody
    • K RAS2A antibody
    • K RAS2B antibody
    • K RAS4A antibody
    • K RAS4B antibody
    • K-RAS antibody
    • KRAS antibody
    • KRAS1 antibody
    • KRAS2 antibody
    • N-RAS antibody
    • N-terminally processed antibody
    • NRAS antibody
    • NRAS1 antibody
    • p21ras antibody
    • RASH_HUMAN antibody
    • RASH1 antibody
    • RASK2 antibody
    • Transforming protein p21 antibody
    • v Ha ras Harvey rat sarcoma viral oncogene homolog antibody
    • v Ki ras2 Kirsten rat sarcoma viral oncogene homolog antibody
    • v ras neuroblastoma RAS viral oncogene homolog antibody
    see all

Images

  • Western blot - HRP Anti-Ras antibody [EPR3255] (ab199557)
    Western blot - HRP Anti-Ras antibody [EPR3255] (ab199557)
    HRP Anti-Ras antibody [EPR3255] (ab199557) at 1/5000 dilution + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa


    Exposure time: 90 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab199557 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • Western blot - HRP Anti-Ras antibody [EPR3255] (ab199557)
    Western blot - HRP Anti-Ras antibody [EPR3255] (ab199557)
    HRP Anti-Ras antibody [EPR3255] (ab199557) at 1/5000 dilution + SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa


    Exposure time: 4 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab199557 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • HRP Anti-Ras antibody [EPR3255] (ab199557)
    HRP Anti-Ras antibody [EPR3255] (ab199557)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (1)

    Publishing research using ab199557? Please let us know so that we can cite the reference in this datasheet.

    ab199557 has been referenced in 1 publication.

    • Tang M  et al. Inhibition effects of Yuxiao San combined with cisplatin on transplanted tumor growths via upregulation of nm-23 and downregulation of K-ras in Lewis lung cancer mice. Oncol Lett 17:1267-1273 (2019). PubMed: 30655894

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