Recombinant HRP Anti-RASA1 antibody [EP536Y] (ab204006)
![Western blot - HRP Anti-RASA1 antibody [EP536Y] (ab204006)](https://www.abcam.com/ps/products/204/ab204006/Images/ab204006-249225-WBab2040061.jpg "Western blot - HRP Anti-RASA1 antibody [EP536Y] (ab204006)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP536Y] to RASA1
- Suitable for: WB
- Reacts with: Rat
- Conjugation: HRP
Overview
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Product name
HRP Anti-RASA1 antibody [EP536Y]
See all RASA1 primary antibodies -
Description
HRP Rabbit monoclonal [EP536Y] to RASA1 -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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Immunogen
Synthetic peptide within Human RASA1 aa 250-350 (internal sequence). The exact sequence is proprietary. From near the SH3 domain.
Database link: P20936 -
Positive control
- WB: Rat brain tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP536Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Rat
|
| All applications |
Mouse
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000. Detects a band of approximately 140,45 kDa (predicted molecular weight: 116 kDa).
|
| Notes |
|---|
|
WB
1/5000. Detects a band of approximately 140,45 kDa (predicted molecular weight: 116 kDa). |
Target
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Function
Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21. -
Tissue specificity
In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level). -
Involvement in disease
Note=Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas.
Defects in RASA1 are the cause of capillary malformation-arteriovenous malformation (CMAVM) [MIM:608354]. CMAVM is a disorder characterized by atypical capillary malformations that are multiple, small, round to oval in shape and pinkish red in color. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome.
Defects in RASA1 are a cause of Parkes Weber syndrome (PKWS) [MIM:608355]. PKWS is a disorder characterized by a cutaneous flush with underlying multiple micro-arteriovenous fistulas, in association with soft tissue and skeletal hypertrophy of the affected limb. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 PH domain.
Contains 1 Ras-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5921 Human
- Entrez Gene: 218397 Mouse
- Entrez Gene: 25676 Rat
- Omim: 139150 Human
- SwissProt: P20936 Human
- SwissProt: P50904 Rat
- Unigene: 664080 Human
- Unigene: 12223 Rat
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Alternative names
- CM AVM antibody
- CMAVM antibody
- DKFZp434N071 antibody
see all
Images
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Western blot - HRP Anti-RASA1 antibody [EP536Y] (ab204006)HRP Anti-RASA1 antibody [EP536Y] (ab204006) at 1/5000 dilution + Brain (Rat) Tissue Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 116 kDa
Observed band size: 140,45 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab204006 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-RASA1 antibody [EP536Y] (ab204006)](https://www.abcam.com/ps/products/204/ab204006/Images/ab204006-3-benefits-of-recombinant-antibodies.png)
HRP Anti-RASA1 antibody [EP536Y] (ab204006)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab204006 has not yet been referenced specifically in any publications.