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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Regulators
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RecombinantRabMAb

Recombinant HRP Anti-RASA1 antibody [EP536Y] (ab204006)

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  • Certificate of Compliance
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Western blot - HRP Anti-RASA1 antibody [EP536Y] (ab204006)
  • HRP Anti-RASA1 antibody [EP536Y] (ab204006)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EP536Y] to RASA1
  • Suitable for: WB
  • Reacts with: Rat
  • Conjugation: HRP

You may also be interested in

Protein
Product image
Recombinant Human RASA1 protein (ab116964)
Primary
Product image
Anti-RASA1 antibody [EP536Y] - BSA and Azide free (ab247281)

View more associated products

Overview

  • Product name

    HRP Anti-RASA1 antibody [EP536Y]
    See all RASA1 primary antibodies
  • Description

    HRP Rabbit monoclonal [EP536Y] to RASA1
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested Applications & Species

    Application Species
    WB
    Rat
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human RASA1 aa 250-350 (internal sequence). The exact sequence is proprietary. From near the SH3 domain.
    Database link: P20936

  • Positive control

    • WB: Rat brain tissue lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP536Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Regulators

Associated products

  • Alternative Versions

    • Anti-RASA1 antibody [EP536Y] - BSA and Azide free (ab247281)
    • Anti-RASA1 antibody [EP536Y] (ab40677)
  • Isotype control

    • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)
  • Recombinant Protein

    • Recombinant Human RASA1 protein (ab116964)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab204006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Rat
All applications
Mouse
Human
Application Abreviews Notes
WB
1/5000. Detects a band of approximately 140,45 kDa (predicted molecular weight: 116 kDa).
Notes
WB
1/5000. Detects a band of approximately 140,45 kDa (predicted molecular weight: 116 kDa).

Target

  • Function

    Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21.
  • Tissue specificity

    In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level).
  • Involvement in disease

    Note=Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas.
    Defects in RASA1 are the cause of capillary malformation-arteriovenous malformation (CMAVM) [MIM:608354]. CMAVM is a disorder characterized by atypical capillary malformations that are multiple, small, round to oval in shape and pinkish red in color. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome.
    Defects in RASA1 are a cause of Parkes Weber syndrome (PKWS) [MIM:608355]. PKWS is a disorder characterized by a cutaneous flush with underlying multiple micro-arteriovenous fistulas, in association with soft tissue and skeletal hypertrophy of the affected limb.
  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 PH domain.
    Contains 1 Ras-GAP domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P20936 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5921 Human
    • Entrez Gene: 218397 Mouse
    • Entrez Gene: 25676 Rat
    • Omim: 139150 Human
    • SwissProt: P20936 Human
    • SwissProt: P50904 Rat
    • Unigene: 664080 Human
    • Unigene: 12223 Rat
    • Alternative names

      • CM AVM antibody
      • CMAVM antibody
      • DKFZp434N071 antibody
      • GAP antibody
      • GTPase activating protein antibody
      • GTPase-activating protein antibody
      • OTTHUMP00000222390 antibody
      • OTTHUMP00000222391 antibody
      • OTTHUMP00000222392 antibody
      • OTTHUMP00000222393 antibody
      • p120GAP antibody
      • p120RASGAP antibody
      • PKWS antibody
      • Ras GTPase-activating protein 1 antibody
      • RAS p21 protein activator (GTPase activating protein) 1 antibody
      • Ras p21 protein activator antibody
      • RASA antibody
      • RASA1 antibody
      • RASA1_HUMAN antibody
      • RasGAP antibody
      • Triphosphatase activating protein antibody
      see all

    Images

    • Western blot - HRP Anti-RASA1 antibody [EP536Y] (ab204006)
      Western blot - HRP Anti-RASA1 antibody [EP536Y] (ab204006)
      HRP Anti-RASA1 antibody [EP536Y] (ab204006) at 1/5000 dilution + Brain (Rat) Tissue Lysate at 10 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 116 kDa
      Observed band size: 140,45 kDa why is the actual band size different from the predicted?


      Exposure time: 20 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab204006 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • HRP Anti-RASA1 antibody [EP536Y] (ab204006)
      HRP Anti-RASA1 antibody [EP536Y] (ab204006)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab204006? Please let us know so that we can cite the reference in this datasheet.

    ab204006 has not yet been referenced specifically in any publications.

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