HRP Anti-RFP antibody (ab34767)

Thank you very much for your interest in ab34767.

To our knowledge, ab34767 has not been tested in tdTomato, however based on sequence homology there is a good chance that it will work. Therefore, I can offer a discount off a future purchase if you buy ab34767 now, test it in tdTomato and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab34767 in tdTomato. I will then send a discount code. This code must be issued before purchasing ab34767 so please wait for my reply before ordering.

2. Purchase ab34767 either by phone, fax, or online (www.abcam.com).

3. Test it in tdTomato.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab34767 turns out to be unsuitable for tdTomato you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Thank you for your reply. I have sent a free vial of ab34767 to replace ab28664. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I have also issued a credit note for ab6722. The credit note may be used in one of the following ways: (1) Redeemed against the original invoice if this hasn't already been paid. (2) Held on the account for use against a future order. (3) A full refund can be offered where no other invoices are outstanding. Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge. To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website. The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

Thank you for your email. I would be happy to send a free vial of ab34767 (to replace ab28664) aswell as credit you for the secondary antibody purchased. Do you have the secondary product code and date of order? I need to find the original order to process the credit. Looking forward to your reply.

Thank you for the further information. My colleague Kate is currently out of the office, so I will be managing this case. I am sorry to hear you have been experincing problems with ab28664. I would be happy to send you a replacment of another product, ab34767 may be of interest as it is conjugated with HRP: Click here (or use the following: https://www.abcam.com/HRP-RFP-antibody-ab34767.html). Please let me know whether you would like to receive this antibody as a replacement. Reviewing the protocol information, I would be careful about storing the supernatant from the protein extraction at 4C, -20 may be the most appropriate storage temperature to prevent protein degradation. It would also be interesting if there is another method to confirm that the proteins are expressed. Do you have a control protein that can be expressed at the same time and testing with another antibody? This would act as a positive control. I look forward to your reply.

Thank you for your enquiry. Yes, ab28664 has been tested for Western blotting and will demonstrate the expression of your DSred fusion protein. But I recommend ab34767 which is an HRP conjugate, removing the need for a secondary detection antibody. It can be used more dilute than ab28664, making it cheaper per blot. It has been tested for ELISA, which will be a more sensitive assay for the purposes of normalization than Western blot densitometry, though Western blot will demonstrate the specificity of the antibody, revealed by a single band for the fusion protein. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your recent technical enquiry. Unfortunately, we do not have information regarding the reactivity of this product with mcherry. It has only been shown to detect RFP from Discosoma. I am sorry I can't be of more assistance in this occasion but should you require any further information, please do not hesitate to contact me.