HRP Anti-RFP antibody (ab34767)
Key features and details
- HRP Rabbit polyclonal to RFP
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-RFP antibody
See all RFP primary antibodies -
Description
HRP Rabbit polyclonal to RFP -
Host species
Rabbit -
Conjugation
HRP -
Specificity
ab34767 is designed to detect RFP and its variants. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Fusion protein The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
immunoaffinity chromatography was followed by solid phase adsorption to remove any unwanted reactivities. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Recombinant Protein
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34767 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000 - 1/5000. Detects a band of approximately 27 kDa.
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| Notes |
|---|
|
WB
1/1000 - 1/5000. Detects a band of approximately 27 kDa. |
Target
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Relevance
Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. -
Alternative names
- DsRed antibody
- GFP like chromoprotein antibody
- red fluorescent protein antibody
- RFP antibody
Images
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Western blot - HRP Anti-RFP antibody (ab34767)All lanes : HRP Anti-RFP antibody (ab34767) at 1/2500 dilution
Lane 1 : HeLa cell extract
Lane 2 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-a-Rabbit IgG [H&L] at 1/5000 dilution
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab34767 has been referenced in 7 publications.
- Hu G et al. Identification of a novel cell-penetrating peptide derived from the capsid protein of chicken anemia virus and its application in gene delivery. Appl Microbiol Biotechnol 104:10503-10513 (2020). PubMed: 33141296
- Xiao Y et al. Mechanisms of RALF peptide perception by a heterotypic receptor complex. Nature 572:270-274 (2019). PubMed: 31291642
- Hennigan RF et al. Proximity biotinylation identifies a set of conformation-specific interactions between Merlin and cell junction proteins. Sci Signal 12:N/A (2019). PubMed: 31015291
- Thevenard J et al. Low-density lipoprotein receptor-related protein-1 mediates endocytic clearance of tissue inhibitor of metalloproteinases-1 and promotes its cytokine-like activities. PLoS One 9:e103839 (2014). WB . PubMed: 25075518
- Chen XW et al. SEC24A deficiency lowers plasma cholesterol through reduced PCSK9 secretion. Elife 2:e00444 (2013). PubMed: 23580231
- Caillaud MC et al. A downy mildew effector attenuates salicylic Acid-triggered immunity in Arabidopsis by interacting with the host mediator complex. PLoS Biol 11:e1001732 (2013). WB . PubMed: 24339748
- Batters C et al. Visualisation of PCNA monoubiquitination in vivo by single pass spectral imaging FRET microscopy. PLoS One 5:e9008 (2010). WB . PubMed: 20126408