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    hrp-rfp-antibody-ab34767.pdf

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Tags & Cell Markers Fusion / Marker Proteins RFP
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HRP Anti-RFP antibody (ab34767)

  • Datasheet
  • SDS
Reviews (3)Q&A (6)References (5)

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Abpromise

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Find out more.

Western blot - HRP Anti-RFP antibody (ab34767)

    Key features and details

    • HRP Rabbit polyclonal to RFP
    • Suitable for: WB
    • Conjugation: HRP
    • Isotype: IgG

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    Overview

    • Product name

      HRP Anti-RFP antibody
      See all RFP primary antibodies
    • Description

      HRP Rabbit polyclonal to RFP
    • Host species

      Rabbit
    • Conjugation

      HRP
    • Specificity

      ab34767 is designed to detect RFP and its variants.
    • Tested applications

      Suitable for: WBmore details
    • Immunogen

      Fusion protein The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      Preservative: 0.01% Gentamicin sulphate
      Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      immunoaffinity chromatography was followed by solid phase adsorption to remove any unwanted reactivities.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Tags & Cell Markers
      • Fusion / Marker Proteins
      • RFP

    Associated products

    • Recombinant Protein

      • Recombinant RFP protein (ab51993)
    • Related Products

      • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
      • TMB ELISA Substrate (High Sensitivity) (ab171523)
      • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
      • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
      • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
      • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
      • 450 nm Stop Solution for TMB Substrate (ab171529)
      • 650 nm Stop Solution for TMB Substrate (ab171531)
      • Immunoassay Blocking Buffer (ab171534)
      • Immunoassay Blocking (BSA Free) (ab171535)

    Applications

    Our Abpromise guarantee covers the use of ab34767 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/1000 - 1/5000. Detects a band of approximately 27 kDa.

    Target

    • Relevance

      Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
    • Alternative names

      • DsRed antibody
      • GFP like chromoprotein antibody
      • red fluorescent protein antibody
      • RFP antibody

    Images

    • Western blot - HRP Anti-RFP antibody (ab34767)
      Western blot - HRP Anti-RFP antibody (ab34767)
      All lanes : HRP Anti-RFP antibody (ab34767) at 1/2500 dilution

      Lane 1 : HeLa cell extract
      Lane 2 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-a-Rabbit IgG [H&L] at 1/5000 dilution

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (5)

    Publishing research using ab34767? Please let us know so that we can cite the reference in this datasheet.

    ab34767 has been referenced in 5 publications.

    • Hennigan RF  et al. Proximity biotinylation identifies a set of conformation-specific interactions between Merlin and cell junction proteins. Sci Signal 12:N/A (2019). PubMed: 31015291
    • Thevenard J  et al. Low-density lipoprotein receptor-related protein-1 mediates endocytic clearance of tissue inhibitor of metalloproteinases-1 and promotes its cytokine-like activities. PLoS One 9:e103839 (2014). WB . PubMed: 25075518
    • Chen XW  et al. SEC24A deficiency lowers plasma cholesterol through reduced PCSK9 secretion. Elife 2:e00444 (2013). PubMed: 23580231
    • Caillaud MC  et al. A downy mildew effector attenuates salicylic Acid-triggered immunity in Arabidopsis by interacting with the host mediator complex. PLoS Biol 11:e1001732 (2013). WB . PubMed: 24339748
    • Batters C  et al. Visualisation of PCNA monoubiquitination in vivo by single pass spectral imaging FRET microscopy. PLoS One 5:e9008 (2010). WB . PubMed: 20126408

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-9 of 9 Abreviews or Q&A

    Western blot abreview for Anti-RFP antibody (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Nicotiana benthamiana Tissue lysate - whole (Total protein extract from infiltrated leaves as i)
    Gel Running Conditions
    Reduced Denaturing (15% acrylamide)
    Loading amount
    20 µg
    Specification
    Total protein extract from infiltrated leaves as i
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Emily Breeze

    Verified customer

    Submitted Sep 28 2017

    Western blot abreview for Anti-RFP antibody (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Escherichia coli Cell lysate - whole cell (E. coli expressing mCherry)
    Gel Running Conditions
    Reduced Denaturing (4-20% gradient gel)
    Loading amount
    30 cells
    Specification
    E. coli expressing mCherry
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 20 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-RFP antibody (HRP)

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Sample
    Mouse Cell (Prostate)
    Specification
    Prostate
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 24 2013

    Question

    Hi,

    I would like to order an antibody to detect the red fluorescent protein. The RFP we are using now is td-tomato. I am not sure if this antibody ab34767 would work for us. Could you please verify this for me? Thank you very much!

    Read More

    Abcam community

    Verified customer

    Asked on Feb 06 2012

    Answer

    Thank you very much for your interest in ab34767.

    To our knowledge, ab34767 has not been tested in tdTomato, however based on sequence homology there is a good chance that it will work. Therefore, I can offer a discount off a future purchase if you buy ab34767 now, test it in tdTomato and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab34767 in tdTomato. I will then send a discount code. This code must be issued before purchasing ab34767 so please wait for my reply before ordering.

    2. Purchase ab34767 either by phone, fax, or online (www.abcam.com).

    3. Test it in tdTomato.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab34767 turns out to be unsuitable for tdTomato you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Feb 06 2012

    Question

    I would like to receive a vial of ab34767 as a refund for my order for ab28664. Also, this is my purchasing code for another antibody I have ordered and that does not work with the replacement product you will send me :Po-IG/101857, order on the 18th August 2011 and for which I would like a refund or credits.   Please advise me how quickly the replacement antibody will arrive, as I would like to proceed with my experiments.   Thank you for your assistance!

    Read More

    Abcam community

    Verified customer

    Asked on Sep 30 2011

    Answer

    Thank you for your reply. I have sent a free vial of ab34767 to replace ab28664. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I have also issued a credit note for ab6722. The credit note may be used in one of the following ways: (1) Redeemed against the original invoice if this hasn't already been paid. (2) Held on the account for use against a future order. (3) A full refund can be offered where no other invoices are outstanding. Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge. To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website. The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

    Read More

    Abcam Scientific Support

    Answered on Sep 30 2011

    Question

    Hi!   Thank you for your reply. I will take on board your suggestions regarding storage of the extracted protein and using a positive control. However, I think that using the ab34767 antibody will greatly speed up and improve proceedings for me, so I would like to receive it as a refund on the ab28664 order. Also, I was wondering if I could get credits or a refund on my other purchase from abcam, a goat secondary antibody- AP conjugated, as this purchase was triggeredd only by me aquiring ab28664.   Thank you!

    Read More

    Abcam community

    Verified customer

    Asked on Sep 23 2011

    Answer

    Thank you for your email. I would be happy to send a free vial of ab34767 (to replace ab28664) aswell as credit you for the secondary antibody purchased. Do you have the secondary product code and date of order? I need to find the original order to process the credit. Looking forward to your reply.

    Read More

    Abcam Scientific Support

    Answered on Sep 23 2011

    Question

    Please find attached the completed questionaire. I am starting to question if a denaturing SDS gel is suitable for separating RFP tagged proteins and then use them to be recognized by the RFP antibody (maybe the conformation changed too much for them to still be recognized by the antibody?..   If you consider not possible to enhance my results, please exchange teh 2 antibodies I ordered from Abcam to a single antibody,  anti RFP AP or HRP conjugated. Thank you!

    Read More

    Abcam community

    Verified customer

    Asked on Sep 21 2011

    Answer

    Thank you for the further information. My colleague Kate is currently out of the office, so I will be managing this case. I am sorry to hear you have been experincing problems with ab28664. I would be happy to send you a replacment of another product, ab34767 may be of interest as it is conjugated with HRP: Click here (or use the following: https://www.abcam.com/HRP-RFP-antibody-ab34767.html). Please let me know whether you would like to receive this antibody as a replacement. Reviewing the protocol information, I would be careful about storing the supernatant from the protein extraction at 4C, -20 may be the most appropriate storage temperature to prevent protein degradation. It would also be interesting if there is another method to confirm that the proteins are expressed. Do you have a control protein that can be expressed at the same time and testing with another antibody? This would act as a positive control. I look forward to your reply.

    Read More

    Abcam Scientific Support

    Answered on Sep 21 2011

    Question

    I am interested in the DS RED antibody ab28664 and am wondering whether it would be useful for my application: I am using DSred in an IRES consruct to act as a transfection control for two variants of a protein of interest.  I want to ba able to compare protein expression between my two variants. However, I see variation in transfection efficiency (DS red intensity) between cells. I am wondering if I can perform a western blot to detect DSred and then use this to normalize for transfection efficiency therefore allowing me to compare expression of my proteins if interest. I am wondering whether it is possible to use DSred antibody for this type of application and would appreciate any advice you can give me.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 26 2007

    Answer

    Thank you for your enquiry. Yes, ab28664 has been tested for Western blotting and will demonstrate the expression of your DSred fusion protein. But I recommend ab34767 which is an HRP conjugate, removing the need for a secondary detection antibody. It can be used more dilute than ab28664, making it cheaper per blot. It has been tested for ELISA, which will be a more sensitive assay for the purposes of normalization than Western blot densitometry, though Western blot will demonstrate the specificity of the antibody, revealed by a single band for the fusion protein. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Sep 26 2007

    Question

    I was wondering if the anti-RFP (HRP conjugated) antibody detect mcherry? Thanks,

    Read More

    Abcam community

    Verified customer

    Asked on Jun 26 2007

    Answer

    Thank you for your recent technical enquiry. Unfortunately, we do not have information regarding the reactivity of this product with mcherry. It has only been shown to detect RFP from Discosoma. I am sorry I can't be of more assistance in this occasion but should you require any further information, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Jun 26 2007

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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