Recombinant HRP Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab195535)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab195535)](https://www.abcam.com/ps/products/195/ab195535/Images/ab195535-237362-anti-s100-beta-antibody-ep1576y-hrp-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab195535)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP1576Y] to S100 beta - Astrocyte Marker
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker
See all S100 beta primary antibodies -
Description
HRP Rabbit monoclonal [EP1576Y] to S100 beta - Astrocyte Marker -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human cerebellum tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Dissociation constant (KD)
KD = 5.50 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1576Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab195535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. -
Tissue specificity
Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. -
Sequence similarities
Belongs to the S-101 family.
Contains 2 EF-hand domains. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6285 Human
- Entrez Gene: 20203 Mouse
- Entrez Gene: 25742 Rat
- Omim: 176990 Human
- SwissProt: P04271 Human
- SwissProt: P50114 Mouse
- SwissProt: P04631 Rat
- Unigene: 422181 Human
see all -
Alternative names
- NEF antibody
- Protein S100 B antibody
- Protein S100-B antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab195535)
IHC image of S100 beta staining in a section of formalin-fixed paraffin-embedded normal human cerebellum tissue, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195535 at 1/500 dilution, for 15 mins at room temperature.
DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![HRP Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab195535)](https://www.abcam.com/ps/products/195/ab195535/Images/ab195535-2-benefits-of-recombinant-antibodies.png)
HRP Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab195535)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab195535 has not yet been referenced specifically in any publications.