HRP Anti-S6K1 antibody (ab9367)
Key features and details
- HRP Rabbit polyclonal to S6K1
- Suitable for: WB
- Reacts with: Mouse, Rat, Cow, Human, Pig
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-S6K1 antibody
See all S6K1 primary antibodies -
Description
HRP Rabbit polyclonal to S6K1 -
Host species
Rabbit -
Conjugation
HRP -
Specificity
Reacts strongly with the 70 kDa of p70 S6K but only weakly to the 80 kDa S6K. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human, Pig -
Immunogen
Synthetic peptide corresponding to Human S6K1 (C terminal) conjugated to keyhole limpet haemocyanin.
Sequence:C-ISKRPEHLRMNL
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6
Preservative: 0.02% Sodium azide
Constituent: Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Immunoaffinity chromatography with S6K c-terminal peptide on agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Recombinant Protein
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Related Products
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Applications
Our Abpromise guarantee covers the use of ab9367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/250. |
Target
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Function
Acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. -
Domain
The autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.
The TOS (TOR signaling) motif is essential for activation by mTORC1. -
Post-translational
modificationsPhosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism. -
Cellular localization
Cytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane. - Information by UniProt
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Database links
- Entrez Gene: 404181 Cow
- Entrez Gene: 6198 Human
- Entrez Gene: 72508 Mouse
- Entrez Gene: 83840 Rat
- Omim: 608938 Human
- SwissProt: P23443 Human
- SwissProt: Q8BSK8 Mouse
- SwissProt: P67999 Rat
see all -
Alternative names
- 70 kDa ribosomal protein S6 kinase 1 antibody
- KS6B1_HUMAN antibody
- p70 alpha antibody
see all
Images
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Western blot - HRP Anti-S6K1 antibody (ab9367)
One-step immunoblotting with cross-antibodies of the immunoprecipitated kinases
Immunoprecipitation with anti-p90 RSK1 (Lane A and C) Immunoprecipitation with anti-p70 S6K (Lane B and D) Immunoblotting with anti-p70 S6K HRPconjugates (Lane A and B)
Immunoblotting with anti-RSK1 HRP conjugates (Lane C and D)
One-step immunoblotting with cross-antibodies of the immunoprecipitated kinases
Immunoprecipitation with anti-p90 RSK1 (Lane A and C)
Immunoprecipitation with anti-p70 S6K (Lane B and D)
Immunoblotting with anti-p70 S6K HRPconjugates (Lane A and B)
Immunoblotting with anti-RSK1 HRP conjugates (Lane C and D) -
Western blot - HRP Anti-S6K1 antibody (ab9367)One-step immunoblotting of crude mouse brain and muscle samples with anti-S6K HRP conjugates (ECL system).
Lane A: mouse Muscle
Lane B: mouse Brain
One-step immunoblotting of crude mouse brain and muscle samples with anti-S6K HRP conjugates (ECL system).
Lane A: mouse Muscle
Lane B: mouse Brain
Datasheets and documents
References (0)
ab9367 has not yet been referenced specifically in any publications.
