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  1. Link

    hrp-s6k1-antibody-ab9367.pdf

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Cell Biology Cell Cycle Cell Cycle Inhibitors Rb
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HRP Anti-S6K1 antibody (ab9367)

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Western blot - HRP Anti-S6K1 antibody (ab9367)
  • Western blot - HRP Anti-S6K1 antibody (ab9367)

Key features and details

  • HRP Rabbit polyclonal to S6K1
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Cow, Human, Pig
  • Conjugation: HRP
  • Isotype: IgG

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Overview

  • Product name

    HRP Anti-S6K1 antibody
    See all S6K1 primary antibodies
  • Description

    HRP Rabbit polyclonal to S6K1
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Specificity

    Reacts strongly with the 70 kDa of p70 S6K but only weakly to the 80 kDa S6K.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Human S6K1 (C terminal) conjugated to keyhole limpet haemocyanin.
    Sequence:

    C-ISKRPEHLRMNL

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 6
    Preservative: 0.02% Sodium azide
    Constituent: Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Immunoaffinity chromatography with S6K c-terminal peptide on agarose.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Rb
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • DNA Damage Recognition
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • S6K Family
    • Metabolism
    • Types of disease
    • Obesity

Associated products

  • Recombinant Protein

    • Recombinant human S6K1 protein (ab62291)
  • Related Products

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 450 nm Stop Solution for TMB Substrate (ab171529)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)

Applications

Our Abpromise guarantee covers the use of ab9367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250.

Target

  • Function

    Acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • Domain

    The autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.
    The TOS (TOR signaling) motif is essential for activation by mTORC1.
  • Post-translational
    modifications

    Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism.
  • Cellular localization

    Cytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane.
  • Target information above from: UniProt accession P23443 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 404181 Cow
    • Entrez Gene: 6198 Human
    • Entrez Gene: 72508 Mouse
    • Entrez Gene: 83840 Rat
    • Omim: 608938 Human
    • SwissProt: P23443 Human
    • SwissProt: Q8BSK8 Mouse
    • SwissProt: P67999 Rat
    • Unigene: 463642 Human
    • Unigene: 394280 Mouse
    • Unigene: 446624 Mouse
    • Unigene: 479484 Mouse
    • Unigene: 4042 Rat
    see all
  • Alternative names

    • 70 kDa ribosomal protein S6 kinase 1 antibody
    • KS6B1_HUMAN antibody
    • p70 alpha antibody
    • P70 beta 1 antibody
    • p70 ribosomal S6 kinase alpha antibody
    • p70 ribosomal S6 kinase beta 1 antibody
    • p70 S6 kinase alpha antibody
    • P70 S6 Kinase antibody
    • p70 S6 kinase, alpha 1 antibody
    • p70 S6 kinase, alpha 2 antibody
    • p70 S6K antibody
    • p70 S6K-alpha antibody
    • p70 S6KA antibody
    • p70(S6K) alpha antibody
    • p70(S6K)-alpha antibody
    • p70-alpha antibody
    • p70-S6K 1 antibody
    • p70-S6K antibody
    • P70S6K antibody
    • P70S6K1 antibody
    • p70S6Kb antibody
    • PS6K antibody
    • Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody
    • Ribosomal protein S6 kinase beta 1 antibody
    • Ribosomal protein S6 kinase beta-1 antibody
    • Ribosomal protein S6 kinase I antibody
    • RPS6KB1 antibody
    • S6K antibody
    • S6K-beta-1 antibody
    • S6K1 antibody
    • Serine/threonine kinase 14 alpha antibody
    • Serine/threonine-protein kinase 14A antibody
    • STK14A antibody
    see all

Images

  • Western blot - HRP Anti-S6K1 antibody (ab9367)
    Western blot - HRP Anti-S6K1 antibody (ab9367)

    One-step immunoblotting with cross-antibodies of the immunoprecipitated kinases

    Immunoprecipitation with anti-p90 RSK1 (Lane A and C) Immunoprecipitation with anti-p70 S6K (Lane B and D) Immunoblotting with anti-p70 S6K HRPconjugates (Lane A and B)

    Immunoblotting with anti-RSK1 HRP conjugates (Lane C and D)

    One-step immunoblotting with cross-antibodies of the immunoprecipitated kinases
    Immunoprecipitation with anti-p90 RSK1 (Lane A and C)
    Immunoprecipitation with anti-p70 S6K (Lane B and D)
    Immunoblotting with anti-p70 S6K HRPconjugates (Lane A and B)
    Immunoblotting with anti-RSK1 HRP conjugates (Lane C and D)
  • Western blot - HRP Anti-S6K1 antibody (ab9367)
    Western blot - HRP Anti-S6K1 antibody (ab9367)

    One-step immunoblotting of crude mouse brain and muscle samples with anti-S6K HRP conjugates (ECL system).

    Lane A: mouse Muscle

    Lane B: mouse Brain

    One-step immunoblotting of crude mouse brain and muscle samples with anti-S6K HRP conjugates (ECL system).
    Lane A: mouse Muscle
    Lane B: mouse Brain

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab9367? Please let us know so that we can cite the reference in this datasheet.

    ab9367 has not yet been referenced specifically in any publications.

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