HRP Anti-SNX27 antibody [1C6] (ab203648)
![Western blot - HRP Anti-SNX27 antibody [1C6] (ab203648)](https://www.abcam.com/ps/products/203/ab203648/Images/ab203648-248503-WBab2036481.jpg "Western blot - HRP Anti-SNX27 antibody [1C6] (ab203648)")
Key features and details
- HRP Mouse monoclonal [1C6] to SNX27
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-SNX27 antibody [1C6]
See all SNX27 primary antibodies -
Description
HRP Mouse monoclonal [1C6] to SNX27 -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Monkey
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Immunogen
Fusion protein within Human SNX27 (N terminal). The exact sequence is proprietary.
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Epitope
N-terminal amino acids 1-267. -
Positive control
- WB: HeLa, HEK293 and MDA-MB-231 whole cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
1C6 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab203648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
Target
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Function
Involved in endocytic trafficking (By similarity). In T lymphocytes, participates in endocytic recycling pathway. Recruits PSCDBP and HT4R to early endosomes. -
Tissue specificity
Expressed in cells of hematopoietic origin (at protein level). -
Sequence similarities
Belongs to the sorting nexin family.
Contains 1 PDZ (DHR) domain.
Contains 1 PX (phox homology) domain.
Contains 1 Ras-associating domain. -
Domain
The PDZ domain mediates the interaction with DGKZ, PSCDBP and HT4R and is responsible for vesicular localization. -
Cellular localization
Cytoplasm > cytosol. Early endosome. In T-lymphocytes, recruited from the cytosol to sorting endosomes by phosphoinositide-3-kinase products. - Information by UniProt
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Database links
- Entrez Gene: 81609 Human
- Omim: 611541 Human
- SwissProt: Q96L92 Human
- Unigene: 192326 Human
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Alternative names
- KIAA0488 antibody
- Methamphetamine responsive transcript 1 antibody
- MGC126871 antibody
see all
Images
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Western blot - HRP Anti-SNX27 antibody [1C6] (ab203648)All lanes : HRP Anti-SNX27 antibody [1C6] (ab203648) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab203648 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
References (0)
ab203648 has not yet been referenced specifically in any publications.
