Recombinant HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab185065)
![Western blot - HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab185065)](https://www.abcam.com/ps/products/185/ab185065/Images/ab185065-218716-anti-sodium-potassium-atpase-antibody-ep1845y-plasma-membrane-loading-control-hrp-western-blot.jpg "Western blot - HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab185065)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP1845Y] to Sodium Potassium ATPase - Plasma Membrane Loading Control
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control
See all Sodium Potassium ATPase primary antibodies -
Description
HRP Rabbit monoclonal [EP1845Y] to Sodium Potassium ATPase - Plasma Membrane Loading Control -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Tilapia
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Immunogen
Synthetic peptide within Human Sodium Potassium ATPase aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- This antibody gave a positive signal in human stomach carcinoma tissue lysate as well as the following whole cell lysates: HeLa; HEK293 IHC: Human brain (cerebral cortex), rat brain and mouse brain.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1845Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Sodium Potassium ATPase antibody [EP1845Y] - BSA and Azide free (ab167390)
- Alexa Fluor® 488 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (ab197713)
- Biotin Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab198366)
- Alexa Fluor® 647 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (ab198367)
- PE Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab209299)
- Alexa Fluor® 405 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab210143)
- Alexa Fluor® 555 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab274883)
- Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab76020)
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab185065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ab199507 - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody.
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| WB | 1/5000. Predicted molecular weight: 113 kDa. |
Target
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Function
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. -
Post-translational
modificationsPhosphorylation on Tyr-10 modulates pumping activity. -
Cellular localization
Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 476 Human
- Entrez Gene: 480 Human
- Entrez Gene: 481 Human
- Entrez Gene: 11928 Mouse
- Entrez Gene: 11931 Mouse
- Entrez Gene: 27222 Mouse
- Entrez Gene: 24211 Rat
- Entrez Gene: 25650 Rat
see all -
Alternative names
- ATPase Na+/K+ transporting alpha antibody
- adenosinetriphosphatase antibody
- AT1A1_HUMAN antibody
see all
Images
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Western blot - HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab185065)All lanes : HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab185065) at 1/5000 dilution (Milk blocking 3%)
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 113 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab185065 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab185065)](https://www.abcam.com/ps/products/185/ab185065/Images/ab185065-233513-anti-sodium-potassium-atpase-antibody-ep1845y-plasma-membrane-loading-control-hrp-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab185065)IHC image of Sodium Potassium ATPase staining in a section of formalin-fixed paraffin-embedded Human brain (cerebral cortex). The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab185065, 1µg/ml overnight at +4°C. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab185065)](https://www.abcam.com/ps/products/185/ab185065/Images/ab185065-249315-anti-sodium-potassium-atpase-antibody-ep1845y-plasma-membrane-loading-control-hrp-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab185065)IHC image of Sodium Potassium ATPase staining in a section of formalin-fixed paraffin-embedded normal mouse brain, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab185065, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset background control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab185065)](https://www.abcam.com/ps/products/185/ab185065/Images/ab185065-249314-anti-sodium-potassium-atpase-antibody-ep1845y-plasma-membrane-loading-control-hrp-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab185065)IHC image of Sodium Potassium ATPase staining in a section of formalin-fixed paraffin-embedded normal rat brain, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab185065, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset background control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
-
![HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab185065)](https://www.abcam.com/ps/products/185/ab185065/Images/ab185065-5-benefits-of-recombinant-antibodies.png)
HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab185065)
Protocols
References (4)
ab185065 has been referenced in 4 publications.
- Zhao S et al. High frequency of CD74 expression in lymphomas: implications for targeted therapy using a novel anti-CD74-drug conjugate. J Pathol Clin Res 5:12-24 (2019). PubMed: 30191677
- Choi EJ et al. Gambogic Acid and Its Analogs Inhibit Gap Junctional Intercellular Communication. Front Pharmacol 9:814 (2018). PubMed: 30104974
- Marino A et al. S1P receptor 1-Mediated Anti-Renin-Angiotensin System Cardioprotection: Pivotal Role of Mast Cell Aldehyde Dehydrogenase Type 2. J Pharmacol Exp Ther 362:230-242 (2017). PubMed: 28500264
- Burgess KA et al. Western blot analysis of cells encapsulated in self-assembling peptide hydrogels. Biotechniques 63:253-260 (2017). PubMed: 29235971
