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Validated using a knockout cell lineRecombinantRabMAb

Recombinant HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)

Submit a review Submit a question References (2)
Western blot - HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)
  • Western blot - HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)
  • HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR4844] to SQSTM1 / p62
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human
  • Conjugation: HRP

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free PE Unconjugated

Overview

  • Product name

    HRP Anti-SQSTM1 / p62 antibody [EPR4844]
    See all SQSTM1 / p62 primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR4844] to SQSTM1 / p62

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF-7 and HeLa whole cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab194720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
Notes
WB
1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).

Target

  • Function

    Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels.

  • Tissue specificity

    Ubiquitously expressed.

  • Involvement in disease

    Defects in SQSTM1 are a cause of Paget disease of bone (PDB) [MIM:602080]. PDB is a metabolic bone disease affecting the axial skeleton and characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Manifestations of the disease include bone pain, deformity, pathological fractures, deafness, neurological complications and increased risk of osteosarcoma. PDB is a chronic disease affecting 2 to 3% of the population above the age of 40 years.

  • Sequence similarities

    Contains 1 OPR domain.
    Contains 1 UBA domain.
    Contains 1 ZZ-type zinc finger.

  • Domain

    The UBA domain binds specifically 'Lys-63'-linked polyubiquitin chains of polyubiquitinated substrates. Mediates the interaction with TRIM55.
    The OPR domain mediates homooligomerization and interactions with PRKCZ, PRKCI, MAP2K5 and NBR1.
    The ZZ-type zinc finger mediates the interaction with RIPK1.

  • Post-translational
    modifications

    Phosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN.

  • Cellular localization

    Cytoplasm. Late endosome. Nucleus. Sarcomere (By similarity). In cardiac muscles localizes to the sarcomeric band (By similarity). Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • A170 antibody
    • DMRV antibody
    • EBI 3 associated protein of 60 kDa antibody
    • EBI 3 associated protein p60 antibody
    • EBI3 associated protein of 60 kDa antibody
    • EBI3 associated protein p60 antibody
    • EBI3-associated protein of 60 kDa antibody
    • EBIAP antibody
    • FTDALS3 antibody
    • MGC127197 antibody
    • ORCA antibody
    • OSF-6 antibody
    • Osi antibody
    • OSIL antibody
    • Oxidative stress induced like antibody
    • p60 antibody
    • p62 antibody
    • p62B antibody
    • Paget disease of bone 3 antibody
    • PDB 3 antibody
    • PDB3 antibody
    • Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa antibody
    • Phosphotyrosine independent ligand for the Lck SH2 domain p62 antibody
    • Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa antibody
    • PKC-zeta-interacting protein antibody
    • Protein kinase C-zeta-interacting protein antibody
    • Sequestosome 1 antibody
    • Sequestosome-1 antibody
    • SQSTM 1 antibody
    • SQSTM_HUMAN antibody
    • Sqstm1 antibody
    • STAP antibody
    • STONE14 antibody
    • Ubiquitin binding protein p62 antibody
    • Ubiquitin-binding protein p62 antibody
    • ZIP 3 antibody
    • ZIP antibody
    • ZIP3 antibody
    see all

Images

  • Western blot - HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)
    Western blot - HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)
    All lanes : HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : SQSTM1 knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 62 kDa


    Exposure time: 12 minutes


    ab194720 was shown to specifically react with SQSTM1 in wild-type HAP1 cells as signal was lost in SQSTM1 knockout cells. Wild-type and SQSTM1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab194720 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

  • Western blot - HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)
    Western blot - HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)
    All lanes : HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720) at 1/1000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa whole cell lysate (ab150035)

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 62 kDa
    Observed band size: 62 kDa


    Exposure time: 20 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab194720 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)
    HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)

References (2)

Publishing research using ab194720? Please let us know so that we can cite the reference in this datasheet.

ab194720 has been referenced in 2 publications.

  • Li D  et al. ATG14 and RB1CC1 play essential roles in maintaining muscle homeostasis. Autophagy N/A:1-10 (2021). PubMed: 33794726
  • Wang B  et al. ULK1 and ULK2 Regulate Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97. Mol Cell 74:742-757.e8 (2019). PubMed: 30979586

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