Recombinant HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)
![Western blot - HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)](https://www.abcam.com/ps/products/194/ab194720/Images/ab194720-355471-rabbit-monoclonal-epr4844-to-sqstm1-p62-hrp-western-blot-knockout-hap1-sqstm1-human-lysates.jpg "Western blot - HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR4844] to SQSTM1 / p62
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
-
Product name
HRP Anti-SQSTM1 / p62 antibody [EPR4844]
See all SQSTM1 / p62 primary antibodies -
Description
HRP Rabbit monoclonal [EPR4844] to SQSTM1 / p62 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human SQSTM1/ p62 aa 400 to the C-terminus.
-
Positive control
- WB: MCF-7 and HeLa whole cell lysates. IHC: normal human spleen tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4844 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
- Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (ab109012)
- Alexa Fluor® 488 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (ab185015)
- Alexa Fluor® 647 Anti-SQSTM1 / p62 antibody [EPR4844] (ab194721)
- Alexa Fluor® 594 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (ab203429)
- Alexa Fluor® 555 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (ab203430)
- Anti-SQSTM1 / p62 antibody [EPR4844] - BSA and Azide free (ab219581)
- APC Anti-SQSTM1 / p62 antibody [EPR4844] (ab225093)
- PE Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (ab225094)
- Autophagy Analysis (ATG16L1, ATG16L1 pS278, SQSTM1, LC3B, Ubiquitin, M6PR) Antibody Sampler Panel (ab269811)
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab194720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). |
Target
-
Function
Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. -
Tissue specificity
Ubiquitously expressed. -
Involvement in disease
Defects in SQSTM1 are a cause of Paget disease of bone (PDB) [MIM:602080]. PDB is a metabolic bone disease affecting the axial skeleton and characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Manifestations of the disease include bone pain, deformity, pathological fractures, deafness, neurological complications and increased risk of osteosarcoma. PDB is a chronic disease affecting 2 to 3% of the population above the age of 40 years. -
Sequence similarities
Contains 1 OPR domain.
Contains 1 UBA domain.
Contains 1 ZZ-type zinc finger. -
Domain
The UBA domain binds specifically 'Lys-63'-linked polyubiquitin chains of polyubiquitinated substrates. Mediates the interaction with TRIM55.
The OPR domain mediates homooligomerization and interactions with PRKCZ, PRKCI, MAP2K5 and NBR1.
The ZZ-type zinc finger mediates the interaction with RIPK1. -
Post-translational
modificationsPhosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN. -
Cellular localization
Cytoplasm. Late endosome. Nucleus. Sarcomere (By similarity). In cardiac muscles localizes to the sarcomeric band (By similarity). Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma. - Information by UniProt
-
Database links
- Entrez Gene: 8878 Human
- Entrez Gene: 18412 Mouse
- Entrez Gene: 113894 Rat
- Omim: 601530 Human
- SwissProt: Q13501 Human
- SwissProt: Q64337 Mouse
- SwissProt: O08623 Rat
- Unigene: 709030 Human
see all -
Alternative names
- A170 antibody
- DMRV antibody
- EBI 3 associated protein of 60 kDa antibody
see all
Images
-
Western blot - HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)All lanes : HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : SQSTM1 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 62 kDa
Exposure time: 12 minutesab194720 was shown to specifically react with SQSTM1 in wild-type HAP1 cells as signal was lost in SQSTM1 knockout cells. Wild-type and SQSTM1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab194720 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
-
![Western blot - HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)](https://www.abcam.com/ps/products/194/ab194720/Images/ab194720-238648-anti-sqstm1-p62-antibody-epr4844-hrp-western-blot.jpg)
Western blot - HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)All lanes : HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720) at 1/1000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 :HeLa whole cell lysate (ab150035)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 20 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab194720 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)](https://www.abcam.com/ps/products/194/ab194720/Images/ab194720-237353-anti-sqstm1-p62-antibody-epr4844-hrp-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)IHC image of SQSTM1 / p62 staining in a section of formalin-fixed paraffin-embedded normal human spleen tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab194720 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
-
![HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)](https://www.abcam.com/ps/products/194/ab194720/Images/ab194720-4-benefits-of-recombinant-antibodies.png)
HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)
Protocols
References (1)
ab194720 has been referenced in 1 publication.
- Wang B et al. ULK1 and ULK2 Regulate Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97. Mol Cell 74:742-757.e8 (2019). PubMed: 30979586
